Improved shelf‐life of rice bran by domestic heat processing and assessment of its dietary consumption in experimental rats

Rice bran, a valuable byproduct of the rice milling industry, in spite of being a rich nutrient, has limitations in food application, being highly susceptible to rancidity caused by the inherent enzyme lipase. In the present investigation, three simple heat treatment procedures, namely pan roasting, pan roasting with oil and microwave roasting, were examined to extend the shelf-life of three varieties of rice bran. In the heat-treated rice brans, the increase in free fatty acid content at the end of a 3-month storage period was 1.6- to 2.5- fold as a result of heat treatment, while it was 12- to 23-fold in the absence of heat treatment. In an animal experiment, pan-roasted rice bran was fed to rats at 5% and 10% levels to study the palatability and safety of its consumption. The mean food intake was slightly higher in the 10% rice bran group (13.57 ± 0.2 g compared to 12.31 ± 0.52 g in control). Rats in the 10% rice bran group gained higher body weight when compared to the control group, which can be attributed to the higher food consumption by these animals. Dietary rice bran did not have any adverse effect on any of the hematological parameters. Serum protein level was similar to that of the control group except for an increase in serum albumin in rice bran-fed groups, which is indicative of liver sufficiency in rice bran treatment. The weights of liver, kidney, heart and testes were not affected by rice bran either at the 5% or 10% level. It is inferred that simple domestic heat treatment procedures such as pan roasting and microwave heating can effectively check the rancidity in rice bran for a considerable period of storage, making it suitable for possible animal/human consumption.

[1]  M. Sugano,et al.  Rice bran oil and cholesterol metabolism. , 1997, The Journal of nutrition.

[2]  J. Tao,et al.  Rice Bran Stabilization by Improved Internal and External Heating Methods. , 1989 .

[3]  S. Grundy,et al.  Plant sterols as cholesterol-lowering agents: clinical trials in patients with hypercholesterolemia and studies of sterol balance. , 1977, Atherosclerosis.

[4]  W. James,et al.  A life course approach to diet, nutrition and the prevention of chronic diseases , 2004, Public Health Nutrition.

[5]  C. Berset,et al.  Use of a Free Radical Method to Evaluate Antioxidant Activity , 1995 .

[6]  L. Packer,et al.  Isolation and identification of novel tocotrienols from rice bran with hypocholesterolemic, antioxidant, and antitumor properties. , 2000, Journal of agricultural and food chemistry.

[7]  E. H. Cohen Comparison of the official AOAC method with rapid methods for the analysis of moisture in meats. , 1971 .

[8]  G. W. Snedecor Statistical Methods , 1964 .

[9]  J. M. Randall,et al.  Rice Bran Stabilization by Extrusion Cooking for Extraction of Edible Oil , 2006 .

[10]  Fatemeh Malekian,et al.  Lipase and Lipoxygenase Activity, Functionality, And Nutrient Losses in Rice Bran During Storage , 2000 .

[11]  J. V. Prabhakar,et al.  Oryzanol content of Indian rice bran oil and its extraction from soap stock , 1986 .

[12]  F. I. Chow,et al.  Cholesterol-lowering properties of rice bran , 1994 .

[13]  J. V. Prabhakar,et al.  A simple chemical method for stabilization of rice bran , 1986 .

[14]  F. I. Chow,et al.  Cholesterol-Lowering Effects of Rice Bran and Rice Bran Oil Fractions in Hypercholesterolemic Hamsters , 1992 .

[15]  W. Prinyawiwatkul,et al.  Effects of microwave heat, packaging, and storage temperature on fatty acid and proximate compositions in rice bran. , 2000, Journal of agricultural and food chemistry.

[16]  N. O'Brien,et al.  Investigation of the potential genotoxicity of cholesterol oxidation products in two mammalian fibroblast cell lines. , 1998, Nutrition and cancer.

[17]  L. Rudel,et al.  Determination of cholesterol using o-phthalaldehyde. , 1973, Journal of lipid research.

[18]  J. Cornelius Rice bran oil for edible purposes: a review. , 1980 .

[19]  Å. Hansen,et al.  Antioxidant effects of phenolic rye (Secale cereale L.) extracts, monomeric hydroxycinnamates, and ferulic acid dehydrodimers on human low-density lipoproteins. , 2001, Journal of agricultural and food chemistry.

[20]  F. I. Chow,et al.  Cholesterol Response and Foam Cell Formation in Hamsters Fed Rice Bran, Oat Bran, and Cellulose + Soy Protein Diets With or Without Added Vitamin E , 1999 .

[21]  R. Saunders,et al.  Review of rice bran stabilization systems with emphasis on extrusion cooking [to prevent oil breakdown also helps to control microorganism growth and insect populations]. , 1982 .

[22]  Zhimin Xu,et al.  Antioxidant activity of tocopherols, tocotrienols, and gamma-oryzanol components from rice bran against cholesterol oxidation accelerated by 2,2'-azobis(2-methylpropionamidine) dihydrochloride. , 2001, Journal of agricultural and food chemistry.

[23]  G. S. Seetharamaiah,et al.  Studies on hypocholesterolemic activity of rice bran oil. , 1989, Atherosclerosis.

[24]  D. Morel,et al.  Cellular biochemistry of oxysterols derived from the diet or oxidation in vivo , 1996 .

[25]  S. Grundy,et al.  Interruption of the enterohepatic circulation of bile acids in man: comparative effects of cholestyramine and ileal exclusion on cholesterol metabolism. , 1971, The Journal of laboratory and clinical medicine.

[26]  M. Sugano,et al.  Rice bran oil and human health. , 1996, Biomedical and environmental sciences : BES.

[27]  L. Horlick,et al.  Effects of plant sterols oncholesterol metabolism in man , 1976 .

[28]  G. C. Yencho,et al.  Antioxidant activities, phenolic and β-carotene contents of sweet potato genotypes with varying flesh colours , 2007 .