WHOLE‐EMBRYO CULTURE AND THE STUDY OF MAMMALIAN EMBRYOS DURING ORGANOGENESIS

1. Simple and reliable methods are now available for growing rat and mouse embryos in culture at all stages of organogenesis. Primitive‐streak embryos can be maintained for up to 5 days in culture while they develop to early foetal stages. Older embryos are maintained for progressively shorter periods and the most advanced stage that can be supported is equivalent to the rat foetus of 15 days' gestation.

[1]  D. Gaylor,et al.  Reduced interlitter variability in rats resulting from a restricted mating period, and reassessment of the ""litter effect''. , 1976, Teratology.

[2]  E. Jenkinson,et al.  Studies on the immunobiology of mouse fetal membranes: the effect of cell-mediated immunity on yolk sac cells in vitro. , 1974, Journal of reproduction and fertility.

[3]  D. C. Dyer,et al.  Autonomic Receptors of the Early Rat Embryo Heart: Growth and Development 1 , 1976, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[4]  R. Jensh,et al.  The litter effect as a variable in teratologic studies of the albino rat. , 1970, The American journal of anatomy.

[5]  A. Hughes.  DEVELOPMENTAL BIOLOGY AND THE STUDY OF MALFORMATIONS , 1976, Biological reviews of the Cambridge Philosophical Society.

[6]  R. Wales.  Maturation of the mammalian embryo: biochemical aspects. , 1975, Biology of reproduction.

[7]  E. Johnson,et al.  Enzymic differentiation of rat yolk-sac placenta as affected by a teratogenic agent. , 1966, Journal of embryology and experimental morphology.

[8]  J. S. Nicholas,et al.  Development of rat embryos of egg‐cylinder to head‐fold stages in plasma cultures , 1938 .

[9]  A. R. Beaudoin,et al.  Studies on the mechanism of teratogenic action of trypan blue , 1959, The Anatomical record.

[10]  L. J. Smith.  THE EFFECTS OF TRANSECTION AND EXTIRPATION ON AXIS FORMATION AND ELONGATION IN THE YOUNG MOUSE EMBRYO. , 1964, Journal of embryology and experimental morphology.

[11]  R. Halliday,et al.  The route by which passive immunity is transmitted from mother to foetus in the rat , 1956, Proceedings of the Royal Society of London. Series B - Biological Sciences.

[12]  M. Robkin,et al.  The effect of carbon monoxide on glucose metabolism and growth of rat embryos. , 1978, Teratology.

[13]  S. J. Cox,et al.  Metabolite utilization by isolated embryonic rat hearts in vitro. , 1972, Journal of embryology and experimental morphology.

[14]  JOSEPH A. DIPAOLO,et al.  Growth of Explanted Eight Day Hamster Embryos in Circulating Medium , 1968, Nature.

[15]  D. New,et al.  Culture of explanted rat embryos in rotating tubes. , 1973, Journal of reproduction and fertility.

[16]  R. Pedersen,et al.  Morphology of mouse egg cylinder development in vitro: a light and electron microscopic study. , 1977, The Journal of experimental zoology.

[17]  D. Cockroft,et al.  Comparison of growth in vitro and in vivo of post-implantation rat embryos. , 1976, Journal of embryology and experimental morphology.

[18]  D. Salomon,et al.  The relationships between the early mouse embryo and its environment. , 1975, The ... Symposium. Society for Developmental Biology. Symposium.

[19]  E. Jenkinson,et al.  In Vitro Support System for the Study of Blastocyst Differentiation in the Mouse , 1970, Nature.

[20]  T. Shepard,et al.  Studies of the development of congenital anomalies in embryos of riboflavin-deficient, galactoflavin fed rats. II. Role of the terminal elec-tron transport systems. , 1968, Teratology.

[21]  F. Beck.  The teratogenic effects of azo dyes , 1966 .

[22]  N. Škreb.  Température critique provoquant des malformations pendant le développement embryonnaire du rat et ses effets immédiats. , 1965 .

[23]  E. Knoche,et al.  Analysis of intrauterine malformations of the vertebral column induced by oxygen deficiency. , 1959, Canadian Medical Association journal.

[24]  E. Deuchar.  Regeneration of amputated limb-buds in early rat embryos. , 1976, Journal of embryology and experimental morphology.

[25]  The effects of hyperthermia on pregnancy and prenatal development. , 1974, Experimental embryology and teratology.

[26]  E. Johnson,et al.  Respiratory changes observed in abnormally developing rat embryos. , 1968, Teratology.

[27]  J. Lloyd,et al.  A histochemical and biochemical study of some aspects of placental function in the rat using maternal injection of horseradish peroxidase. , 1967, Journal of anatomy.

[28]  J. S. Nicholas,et al.  The Development of Rat Embryos in Tissue Culture. , 1934, Proceedings of the National Academy of Sciences of the United States of America.

[29]  R. Edwards.  The culture of pre-implantation mammalian embryos. , 1969, Proceedings of the Royal Society of Medicine.

[30]  A. Craig.  Catalog of Teratogenic Agents , 1977, British Journal of Cancer.

[31]  G. Morriss.  Morphogenesis of the malformations induced in rat embryos by maternal hypervitaminosis A. , 1972, Journal of anatomy.

[32]  Emeric Törö.  The homeogenetic induction of neural folds in rat embryos , 1938 .

[33]  R. Comline,et al.  Placental transfer of blood gases. , 1975, British medical bulletin.

[34]  C. Grabowski.  A quantitative study of the lethal and teratogenic effects of hypoxia on the three-day chick embryo. , 1961, The American journal of anatomy.

[35]  J. G. Chamberlain,et al.  Direct effects of 6-aminonicotinamide on the developing rat embryo in vitro and in vivo. , 1968, Teratology.

[36]  J. Biggers,et al.  Physiological aspects of growth and development of the preimplantation mammalian embryo. , 1976, Annual review of physiology.

[37]  R. Cole,et al.  Regulation of pre-natal haemopoiesis: evidence for negative feedback control of erythropoiesis in the foetal mouse. , 1977, Journal of embryology and experimental morphology.

[38]  T. Shepard,et al.  Studies of the development of congenital anomalies in embryos of riboflavin-deficient, galactoflavin fed rats. I. Growth and embryologic pathology. , 1968, Teratology.

[39]  Morriss Gm.  Morphogenesis of the malformations induced in rat embryos by maternal hypervitaminosis A. , 1972 .

[40]  E. Deuchar.  The mechanism of axial rotation in the rat embryo: an experimental study in vitro. , 1971, Journal of embryology and experimental morphology.

[41]  G. Morriss,et al.  Regional differences in mesenchymal cell morphology and glycosaminoglycans in early neural-fold stage rat embryos. , 1978, Journal of embryology and experimental morphology.

[42]  E. Deuchar.  Reconstitutive ability of axial tissue in early rat embryos after operations and culture in vitro. , 1975, Journal of embryology and experimental morphology.

[43]  T. Shepard,et al.  Autonomic drug effects on the heart rate of early rat embryos. , 1974, Teratology.

[44]  D. Solter,et al.  Early mouse embryos: growth and differentiation in vitro. , 1974, Experimental cell research.

[45]  D. Kochhar,et al.  Biosynthesis of DNA, RNA and proteins by mouse embryos cultured in the presence of a teratogenic dose of chlorambucil. , 1976, Journal of embryology and experimental morphology.

[46]  D. New.  Development of rat embryos cultured in blood sera. , 1966, Journal of reproduction and fertility.

[47]  R. Cole,et al.  The effects of erythropoietin on haem synthesis in mouse yolk sac and cultured foetal liver cells. , 1966, Journal of embryology and experimental morphology.

[48]  A. Diplock,et al.  The effect of vitamin E and synthetic antioxidants on the growth in vitro of explanted rat embryos. , 1974, Journal of reproduction and fertility.

[49]  H. A. Padykula.  A histochemical and quantitative study of enzymes of the rat's placenta. , 1958, Journal of anatomy.

[50]  D. Cockroft.  Comparison of in vitro and in vivo development of rat foetuses. , 1976, Developmental biology.

[51]  D. Cockroft,et al.  Improved development of head-fold rat embryos in culture resulting from low oxygen and modifications of the culture serum , 1976 .

[52]  U. Murakami,et al.  Vertebral Malformation in the Mouse Foetus Caused by Maternal Hypoxia During Early Stages of Pregnancy , 1963 .

[53]  R. Brent,et al.  Production of congenital malformations using tissue antisera. , 1966, Developmental biology.

[54]  T. Shepard,et al.  A new in vitro culture technique for rat embryos. , 1972, Teratology.

[55]  I. Tygstrup,et al.  CONGENITAL MALFORMATIONS IN NEWBORN INFANTS OF DIABETIC WOMEN. CORRELATION WITH MATERNAL DIABETIC VASCULAR COMPLICATIONS. , 1964, Lancet.

[56]  J. Lloyd,et al.  Inhibition of pinocytosis in rat yolk sac by trypan blue. , 1976, Teratology.

[57]  C. Steele,et al.  Serum variants causing the formation of double hearts and other abnormalities in explanted rat embryos. , 1974, Journal of embryology and experimental morphology.

[58]  D. Kochhar,et al.  Direct exposure of postimplantation mouse embryos to 5-bromodeoxyuridine in vitro and its effect on subsequent chondrogenesis in the limbs. , 1976, Journal of embryology and experimental morphology.

[59]  R. Brent,et al.  The production of congenital malformations using tissue antisera. VII. Yolk-sac antiserum. , 1971, Teratology.

[60]  D. New.  Studies on Mammalian Fetuses in vitro During the Period of Organogenesis , 1973 .

[61]  Arthur H. Smith,et al.  Metabolic rate of rat fetuses in vitro , 1943 .

[62]  E. Jenkinson,et al.  Blastocyst differentiation in vitro. , 1974, Journal of reproduction and fertility.

[63]  D. New,et al.  CULTIVATION OF POST-IMPLANTATION MOUSE AND RAT EMBRYOS ON PLASMA CLOTS. , 1964, Journal of embryology and experimental morphology.

[64]  G. Burr,et al.  The Anti-Sterility Vitamine Fat Soluble E. , 1925, Proceedings of the National Academy of Sciences of the United States of America.

[65]  C. M. Goss,et al.  The first contractions of the heart in rat embryos , 1938 .

[66]  I. Frank.  Gillman, J., Gilbert, C., Gillman, T., Spence, L: A Preliminary Report on Hydrocephalus, Spina Bifida and Other Congenital Anomalies in the Rat Produced by Trypan Blue , 1950 .

[67]  V. Ferm.  Teratogenic Effects of Hyperbaric Oxygen.∗ , 1964, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[68]  C. Steele,et al.  In vitro development of early postimplantation rat embryos. , 1978, Developmental biology.

[69]  L. Dencker.  Trypan blue accumulation in the embryonic gut of rats and mice during the teratogenic phase. , 1977, Teratology.

[70]  R. Schofield,et al.  Effects of Renal Hypoxia on Erythropoietin Production , 1965, British journal of haematology.

[71]  L M Franks.  Organ Culture in Biomedical Research , 1976 .

[72]  D. New,et al.  Development of a placental blood circulation in rat embryos in vitro. , 1977, Journal of embryology and experimental morphology.

[73]  S. J. Cox,et al.  Energy metabolism in isolated rat embryo hearts: effect of metabolic inhibitors. , 1972, Journal of embryology and experimental morphology.

[74]  C. R. Austin.  The Mammalian Fetus in vitro , 1973, Springer US.

[75]  D. Cockroft,et al.  Teratogenic effects of excess glucose on head-fold rat embryos in culture. , 1977, Teratology.

[76]  M. M. Turbow.  Trypan blue induced teratogenesis of rat embryos cultivated in vitro. , 1966, Journal of embryology and experimental morphology.

[77]  C. Steele.  Improved development of "rat egg-cylinders" in vitro as a result of fusion of the heart primordia. , 1972, Nature: New biology.

[78]  D. L. Gunberg.  In vitro development of postimplantation rat embryos cultured on dialyzed rat serum. , 1976, Teratology.

[79]  J. C. Vuletin,et al.  A Light and Electron Microscopic Study , 1976 .

[80]  D. Kochhar.  The use of in vitro procedures in teratology. , 1975, Teratology.

[81]  G. Morriss,et al.  Comparison of the effects of retinol and retinoic acid on postimplantation rat embryos in vitro. , 1977, Teratology.

[82]  C. Berry.  The effect of trypan blue on the growth of the rat embryo in vivo. , 1970, Journal of embryology and experimental morphology.

[83]  T. Shepard,et al.  In vitro study of rat embryos. I. Effects of decreased oxygen on embryonic heart rate. , 1969, Teratology.

[84]  R. Jensh,et al.  Morphologic alterations in the parietal yolk-sac of the rat from the 12th to the 19th day of gestation. , 1977, Journal of embryology and experimental morphology.

[85]  E. Johnson,et al.  Effects of a teratogen (9-methyl pteroylglutamic acid) on oxygen consumption by dispersed embryonic rat cells. , 1972, Teratology.

[86]  S. Clarkson,et al.  Growth of postimplantation mouse embryos cultured in a serum-supplemented, chemically defined medium. , 1969, Teratology.

[87]  T. Shepard,et al.  Methods for detection of environmental agents that produce congenital defects : proceedings of the Guadeloupe Conference , 1975 .

[88]  J. Rash,et al.  Development in vitro of mouse embryos from the two-cell egg stage to the early somite stage. , 1974, Journal of embryology and experimental morphology.

[89]  G. Morriss.  The ultrastructural effects of excess maternal vitamin A on the primitive streak stage rat embryo. , 1973, Journal of embryology and experimental morphology.

[90]  H. A. Padykula,et al.  DIFFERENTIATION OF THE RAT'S YOLK SAC IN ORGAN CULTURE. , 1964, The American journal of anatomy.

[91]  R. Brent,et al.  Effect of yolk-sac antibody on rat embryos grown in culture. , 1972, Journal of embryology and experimental morphology.

[92]  T. Shepard,et al.  Glucose Metabolism By Rat Embryos in Vitro 1 , 1970, Proceedings of the Society for Experimental Biology and Medicine. Society for Experimental Biology and Medicine.

[93]  S. Kreitzer,et al.  Hyperosmolar Nature of Diabetic Coma , 1975, Diabetes.

[94]  C. Legrand,et al.  Différentiation du trophoblaste au cours du développement in vitro d'embryon de Souris , 1971 .

[95]  N. Škreb,et al.  Developmental abnormalities in the rat induced by heat shock. , 1963, Journal of embryology and experimental morphology.

[96]  T. Shepard,et al.  The effect of carbon monoxide on early rat embryo heart rates. , 1976, Environmental research.

[97]  D. Cockroft.  Development in culture of rat foetuses explanted at 12.5 and 13.5 days of gestation. , 1973, Journal of embryology and experimental morphology.

[98]  T. Shepard,et al.  Energy metabolism in early mammalian embryos. , 1970, The ... Symposium. Society for Developmental Biology. Symposium.

[99]  C. M. Goss.  Double hearts produced experimentally in rat embryos , 1935 .

[100]  C. Legrand,et al.  In vitro study of chorionic and ectoplacental trophoblast differentiation in the mouse. , 1975, Journal of embryology and experimental morphology.

[101]  D. New,et al.  Effects of different oxygen concentrations on the development of rat embryos in culture. , 1970, Journal of reproduction and fertility.

[102]  M. Edwards,et al.  GROWTH AND DEVELOPMENT OF THE BRAIN IN NORMAL AND HEAT‐RETARDED GUINEA‐PIGS , 1976 .

[103]  D. Cockroft,et al.  Effects of hyperthermia on rat embryos in culture , 1975, Nature.

[104]  M. Kane,et al.  The effects of inhibitors of energy metabolism on the growth of one-cell rabbit ova to blastocysts in vitro. , 1977, Journal of reproduction and fertility.

[105]  C. Berry.  The effects of an antiserum to the contractile proteins of the heart on the developing rat embryo. , 1971, Journal of embryology and experimental morphology.

[106]  M. Edwards,et al.  Congenital malformations in the rat following induced hyperthermia during gestation. , 1968, Teratology.

[107]  G. Morriss,et al.  The effect of excess vitamin A on the development of rat embryos in culture. , 1974, Journal of embryology and experimental morphology.

[108]  J. S. Nicholas,et al.  The development of rat embryos in a circulating medium , 1938 .

[109]  T. Shepard,et al.  Methods for detecting teratogenic agents in man. , 1976, Environmental health perspectives.

[110]  D. New,et al.  Development of explanted rat fetuses in hyperbaric oxygen. , 1970, Teratology.

[111]  D. New.  Development of explanted rat embryos in circulating medium. , 1967, Journal of embryology and experimental morphology.

[112]  M. Moore,et al.  Ontogeny of the Haemopoietic System: Yolk Sac Origin of In Vivo and In Vitro Colony Forming Cells in the Developing Mouse Embryo * , 1970, British journal of haematology.

[113]  R. Neale.  Effect of moderate carbon-monoxide exposure on fetal development. , 1972, Lancet.

[114]  G. Morriss,et al.  Glycosaminoglycan synthesis in rat embryos during the formation of the primary mesenchyme and neural folds. , 1977, Developmental biology.

[115]  E. Johnson.  Subcellular Effects of Teratogens on Vertebrate Embryos and their Associated Membranes , 1971 .

[116]  J. Yochim.  Development of the progestational uterus: metabolic aspects. , 1975, Biology of reproduction.