Properties of epithelial cells cultured from human carcinomas and nonmalignant tissues.

Human epithelial cell cultures were examined for expression of plasminogen activator and fibronectin matrix. All of the cells examined showed ultrastructural evidence suggesting their epithelial origin, including microvilli and specialized junctions. The nonmalignant cells were also negative for endothelial cell markers (ie. they lacked factor VIII antigen, a nonthrombogenic surface and Weibel-Palade bodies). The nonmalignant lines all produced large amounts of plasminogen activator, whereas the tumor-derived lines showed a gradation of activities, ranging from lines having as much activity as the nonmalignant lines to lines having little or no activity above background. For both normal and malignant cells, addition of dexamethesone only slightly decreased the levels of plasminogen activator. By immunofluorescence microscopy, normal bladder and fetal intestine epithelial cells showed fibronectin in a globular and fibrillar matrix. In contrast, normal mammary epithelial cells had a much diminished amount of fibronectin with a punctate distribution.

[1]  H. Smith.  In vitro properties of epithelial cell lines established from human carcinomas and nonmalignant tissue. , 1979, Journal of the National Cancer Institute.

[2]  E. L. Springer,et al.  Nuclear ultrastructure of epithelial cell lines derived from human carcinomas and nonmalignant tissues. , 1979, Cancer research.

[3]  J. Barrett,et al.  Evidence for the progressive nature of neoplastic transformation in vitro. , 1978, Proceedings of the National Academy of Sciences of the United States of America.

[4]  M. Gershwin,et al.  Enhancement of heterotransplanted human tumor graft survival in nude mice treated with antilymphocyte serum and in congenitally athymic-asplenic (Lasat) mice. , 1978, Journal of the National Cancer Institute.

[5]  P. Nettesheim,et al.  In vitro development of oncogenicity in cell lines established from tracheal epithelium preexposed in vivo to 7,12-dimethylbenz(a)anthracene. , 1978, Cancer research.

[6]  A. Vaheri,et al.  Distribution of a major connective tissue protein, fibronectin, in normal human tissues , 1978, The Journal of experimental medicine.

[7]  R. H. Gray,et al.  Correlation of anchorage-independent growth with tumorigenicity of chemically transformed mouse epidermal cells. , 1978, Cancer research.

[8]  C. Drevon,et al.  Test for malignant transformation of rat liver cells in culture: cytology, growth in soft agar, and production of plasminogen activator. , 1977, Journal of the National Cancer Institute.

[9]  M. Mosesson,et al.  Heterogeneity of the cold-insoluble globulin of human plasma (CIg), a circulating cell surface protein. , 1977, Biochimica et biophysica acta.

[10]  C. Marshall,et al.  Markers of neoplastic transformation in epithelial cell lines derived from human carcinomas. , 1977, Journal of the National Cancer Institute.

[11]  E. L. Springer,et al.  Two syngeneic cell lines from human breast tissue: the aneuploid mammary epithelial (Hs578T) and the diploid myoepithelial (Hs578Bst) cell lines. , 1977, Journal of the National Cancer Institute.

[12]  M. Mosesson,et al.  An improved method for purification of the cold-insoluble globulin of human plasma (CLg). , 1977, Analytical biochemistry.

[13]  K. Sanford,et al.  Colony morphology and growth in agarose as tests for spontaneous neoplastic transformation in vitro. , 1977, Cancer research.

[14]  J. McDougall,et al.  In vitro traits of adenovirus-transformed cell lines and their relevance to tumorigenicity in nude mice , 1977, Cell.

[15]  M. Maines,et al.  Bile pigment formation by skin heme oxygenase: studies on the response of the enzyme to heme compounds and tissue injury , 1977, The Journal of experimental medicine.

[16]  D. Rifkin,et al.  Production of plasminogen activator by established cell lines of mouse origin , 1977, The Journal of cell biology.

[17]  Gary M Williams,et al.  Lack of correlation between plasminogen activating factor production and tumorigenicity in rat liver epithelial cells , 1977 .

[18]  P. Wilson,et al.  Origin and ultrastructure of cells in vitro. , 1977, International review of cytology.

[19]  C. Keysser.  Neoplastic Development, Vol. 2 , 1976 .

[20]  D. Gospodarowicz,et al.  Clonal growth of bovine vascular endothelial cells: fibroblast growth factor as a survival agent. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[21]  J. Vassalli,et al.  Macrophage plasminogen activator: Modulation of enzyme production by anti-inflammatory steroids, mitotic inhibitors, and cyclic nucleotides , 1976, Cell.

[22]  E. L. Springer,et al.  Epithelial cell cultures from normal and cancerous human tissues. , 1976, Journal of the National Cancer Institute.

[23]  R. Hynes,et al.  Surface proteins and fibrinolytic activity of cultured mammalian cells. , 1976, Cancer research.

[24]  D. Rifkin,et al.  PROTEASES PRODUCED BY NORMAL AND MALIGNANT CELLS IN CULTURE , 1976 .

[25]  V. Freedman,et al.  Tumorigenicity of virus-transformed cells in nude mice is correlated specifically with anchorage independent growth in vitro. , 1975, Proceedings of the National Academy of Sciences of the United States of America.

[26]  S. Sorof,et al.  High extracellular fibrinolytic activity of tumors and control normal tissues. , 1975, Biochemical and biophysical research communications.

[27]  J. Pontén.  Neoplastic Human Glia Cells in Culture , 1975 .

[28]  J. Fogh,et al.  New Human Tumor Cell Lines , 1975 .

[29]  J. Folkman,et al.  Fine structure of vascular endothelium in culture. , 1975, Journal of ultrastructure research.

[30]  J. Riggs,et al.  Immunofluorescent studies of RD-114 virus replication in cell culture. , 1974, The Journal of general virology.

[31]  Mosesson Mw,et al.  The cold-insoluble globulin of plasma and its relationship to factor VIII. , 1974 .

[32]  L. Ossowski,et al.  Plasminogen, the serum proenzyme activated by factors from cells transformed by oncogenic viruses. , 1974, The Journal of biological chemistry.

[33]  D. Rifkin,et al.  PROPERTIES OF PLASMINOGEN ACTIVATORS FORMED BY NEOPLASTIC HUMAN CELL CULTURES , 1974, The Journal of experimental medicine.

[34]  P. Majerus,et al.  The binding of thrombin to the surface of human platelets. , 1974, The Journal of biological chemistry.

[35]  A. Long,et al.  A human cell line from a pleural effusion derived from a breast carcinoma. , 1973, Journal of the National Cancer Institute.

[36]  D. Rifkin,et al.  AN ENZYMATIC FUNCTION ASSOCIATED WITH TRANSFORMATION OF FIBROBLASTS BY ONCOGENIC VIRUSES , 1973, The Journal of experimental medicine.

[37]  D. Rifkin,et al.  AN ENZYMATIC FUNCTION ASSOCIATED WITH TRANSFORMATION OF FIBROBLASTS BY ONCOGENIC VIRUSES , 1973, The Journal of experimental medicine.

[38]  H. Smith,et al.  Induction of cell division in medium lacking serum growth factor by SV40. , 1971, Virology.

[39]  M. Mosesson,et al.  The cold-insoluble globulin of human plasma. I. Purification, primary characterization, and relationship to fibrinogen and other cold-insoluble fraction components. , 1970, The Journal of biological chemistry.

[40]  S. Aaronson,et al.  Human sarcoma cells in culture. Identification by colony-forming ability on monolayers of normal cells. , 1970, Experimental cell research.

[41]  S. Aaronson,et al.  Basis for the Acquaisition of Malignant Potential by Mouse Cells Cultivated in vitro , 1968, Science.

[42]  S. Fedoroff.  Proposed usage of animal tissue culture terms. , 1975, Journal of the National Cancer Institute.

[43]  J. Riggs,et al.  Isothiocyanate compounds as fluorescent labeling agents for immune serum. , 1958, The American journal of pathology.