Gene expression in mouse cerebellum during its development.

Genes expressed during the cerebellar development of the mouse were identified in 3'-directed cDNA libraries prepared from the postnatal day 4, day 12, and week 6 cerebellar tissues. Among about 5500 clones selected randomly from each library, there were approximately 3500 distinct species. A total of 7728 species were identified in the three libraries, 1346 of which were known genes in the GenBank, 3041 EST-matching genes, and 3341 new genes. Relative expression levels at the three postnatal stages were quantitated by adapter-tagged competitive PCR for 130 known genes that appeared six times or more in one of the libraries. Genes for ribosomal proteins and some cytoskeletal and nuclear proteins were abundantly expressed at the early stage, coincidently with extensive proliferation of granule cells as the major cerebellar component. Genes related to brain functions, including those for mitochondrial activities and some ion channel systems, were more active at a later stage when the majority of granule cells were engaged in axon extension and synapse formation or the cerebellum had reached maturity. Compared to these stage-specifically expressed genes, genes for transcriptional regulation, signal transduction, protein modification, and basic cellular functions, in general, were not abundantly expressed at any stage of development.

[1]  C. Campagnoni,et al.  Structure and expression of the mouse myelin proteolipid protein gene , 1987, Journal of neuroscience research.

[2]  A. Kerlavage,et al.  Complementary DNA sequencing: expressed sequence tags and human genome project , 1991, Science.

[3]  J. Bantle,et al.  Complexity and characterization of polyadenylated RNA in the mouse brain , 1976, Cell.

[4]  R. Hawkes,et al.  The cloning of zebrin II reveals its identity with aldolase C. , 1994, Development.

[5]  H. Orr,et al.  Sequence of a murine cDNA, pcp-4, that encodes the homolog of the rat brain-specific antigen PEP-19. , 1990, Nucleic acids research.

[6]  C. Finch,et al.  Sequence complexity of polyadenylated RNA obtained from rat brain regions and cultured rat cells of neural origin. , 1978, Biochemistry.

[7]  K Matsubara,et al.  Expression profiles of active genes in human and mouse livers. , 1996, Gene.

[8]  A. Campagnoni,et al.  Identification of a cDNA coding for a fifth form of myelin basic protein in mouse. , 1987, Proceedings of the National Academy of Sciences of the United States of America.

[9]  K. Kinzler,et al.  Serial Analysis of Gene Expression , 1995, Science.

[10]  I. Wool.  Extraribosomal functions of ribosomal proteins. , 1996, Trends in biochemical sciences.

[11]  Kikuya Kato,et al.  Adaptor-tagged competitive PCR: a novel method for measuring relative gene expression. , 1997, Nucleic acids research.

[12]  D. Botstein,et al.  The transcriptional program in the response of human fibroblasts to serum. , 1999, Science.

[13]  J. Claverie,et al.  The significance of digital gene expression profiles. , 1997, Genome research.

[14]  K. Okubo,et al.  A novel system for large-scale sequencing of cDNA by PCR amplification. , 1991, DNA sequence : the journal of DNA sequencing and mapping.

[15]  Kousaku Okubo,et al.  Large scale cDNA sequencing for analysis of quantitative and qualitative aspects of gene expression , 1992, Nature Genetics.

[16]  D. Lipman,et al.  Improved tools for biological sequence comparison. , 1988, Proceedings of the National Academy of Sciences of the United States of America.