SERS-Based Lateral Flow Strip Biosensor for Simultaneous Detection of Listeria monocytogenes and Salmonella enterica Serotype Enteritidis.

Rapid, sensitive, point-of-care detection of bacteria is extremely important in food safety. To address this requirement, we developed a new surface-enhanced Raman scattering (SERS)-based lateral flow (LF) strip biosensor combined with recombinase polymerase amplification (RPA) for simultaneous detection of Listeria monocytogenes and Salmonella enterica serotype Enteritidis. AuMBA@Ag core-shell nanoparticles were used in this SERS-LF. Highly sensitive quantitative detection is achieved by measuring the characteristic peak intensities of SERS tags. Under optimal conditions, the SERS intensities of MBA at 1077 cm-1 on test lines are used to measure S. Enteritidis (y = 1980.6x - 539.3, R2 = 0.9834) and L. monocytogenes (y = 1696.0x - 844, R2 = 0.9889), respectively. The limit of detection is 27 CFU/mL for S. Enteritidis and 19 CFU/mL for L. monocytogenes. Significantly, this SERS-LF has high specificity and applicability in the detection of L. monocytogenes and S. Enteritidis in food samples. Therefore, the SERS-LF is a feasible method for the rapid and quantitative detection of a broad range of bacterial pathogens in real food samples.

[1]  Lingxin Chen,et al.  A SERS-based lateral flow assay biosensor for highly sensitive detection of HIV-1 DNA. , 2016, Biosensors & bioelectronics.

[2]  Gibaek Lee,et al.  Detection of Cronobacter Genus in Powdered Infant Formula by Enzyme-linked Immunosorbent Assay Using Anti-Cronobacter Antibody , 2016, Front. Microbiol..

[3]  Xuewen Lu,et al.  A sensitive lateral flow biosensor for Escherichia coli O157:H7 detection based on aptamer mediated strand displacement amplification. , 2015, Analytica chimica acta.

[4]  A. Aspán,et al.  Comparison of culture, ELISA and PCR techniques for salmonella detection in faecal samples for cattle, pig and poultry , 2007, BMC veterinary research.

[5]  Min-Gon Kim,et al.  An automatic enzyme immunoassay based on a chemiluminescent lateral flow immunosensor. , 2014, Biosensors & bioelectronics.

[6]  Yànfāng Zhāng,et al.  Development of a GeXP-multiplex PCR assay for the simultaneous detection and differentiation of six cattle viruses , 2017, PloS one.

[7]  Zeger Hens,et al.  Development of a Rainbow Lateral Flow Immunoassay for the Simultaneous Detection of Four Mycotoxins. , 2017, Journal of agricultural and food chemistry.

[8]  D. Lim,et al.  Quantitative analysis of thyroid-stimulating hormone (TSH) using SERS-based lateral flow immunoassay , 2017 .

[9]  Mohammed Zourob,et al.  Rapid colorimetric sensing platform for the detection of Listeria monocytogenes foodborne pathogen. , 2016, Biosensors & bioelectronics.

[10]  Hengyi Xu,et al.  Sextuplex PCR combined with immunomagnetic separation and PMA treatment for rapid detection and specific identification of viable Salmonella spp., Salmonella enterica serovars Paratyphi B, Salmonella Typhimurium, and Salmonella Enteritidis in raw meat , 2017 .

[11]  Xiaonan Lu,et al.  Development of a Loop Mediated Isothermal Amplification (LAMP) - Surface Enhanced Raman spectroscopy (SERS) Assay for the Detection of Salmonella Enterica Serotype Enteritidis , 2016, Theranostics.

[12]  Terry J. Smith,et al.  Development and performance evaluation of a recombinase polymerase amplification assay for the rapid detection of group B streptococcus , 2016, BMC Microbiology.

[13]  Feng Xu,et al.  Upconversion nanoparticles based FRET aptasensor for rapid and ultrasenstive bacteria detection. , 2017, Biosensors & bioelectronics.

[14]  S. Low,et al.  Electrophoretic interactions between nitrocellulose membranes and proteins: Biointerface analysis and protein adhesion properties. , 2013, Colloids and surfaces. B, Biointerfaces.

[15]  Rebecca Richards-Kortum,et al.  Multiplexed Recombinase Polymerase Amplification Assay To Detect Intestinal Protozoa. , 2016, Analytical chemistry.

[16]  Hengyi Xu,et al.  Novel strategies to enhance lateral flow immunoassay sensitivity for detecting foodborne pathogens. , 2015, Journal of agricultural and food chemistry.

[17]  Namhyun Choi,et al.  Simultaneous Detection of Dual Nucleic Acids Using a SERS-Based Lateral Flow Assay Biosensor. , 2017, Analytical chemistry.

[18]  J. Mrázek,et al.  Low-fouling surface plasmon resonance biosensor for multi-step detection of foodborne bacterial pathogens in complex food samples. , 2016, Biosensors & bioelectronics.

[19]  Jinhuai Liu,et al.  Three-dimensional hotspots in evaporating nanoparticle sols for ultrahigh Raman scattering: solid-liquid interface effects. , 2015, Nanoscale.

[20]  L. Jaykus,et al.  Development of a Recombinase Polymerase Amplification Assay for Detection of Epidemic Human Noroviruses , 2017, Scientific Reports.

[21]  T. K. Christopoulos,et al.  Identification of single-nucleotide polymorphisms by the oligonucleotide ligation reaction: a DNA biosensor for simultaneous visual detection of both alleles. , 2009, Analytical chemistry.

[22]  J. Choo,et al.  Application of a SERS-based lateral flow immunoassay strip for the rapid and sensitive detection of staphylococcal enterotoxin B. , 2016, Nanoscale.

[23]  K. N. Sood,et al.  Highly sensitive electrochemical immunosensor based on graphene-wrapped copper oxide-cysteine hierarchical structure for detection of pathogenic bacteria , 2017 .

[24]  Pingping Zhang,et al.  Rapid multiplex detection of 10 foodborne pathogens with an up-converting phosphor technology-based 10-channel lateral flow assay , 2016, Scientific Reports.

[25]  Cheng Liu,et al.  Development of a lateral flow colloidal gold immunoassay strip for the simultaneous detection of Shigella boydii and Escherichia coli O157:H7 in bread, milk and jelly samples , 2016 .

[26]  Matt Trau,et al.  Field Demonstration of a Multiplexed Point-of-Care Diagnostic Platform for Plant Pathogens. , 2016, Analytical chemistry.

[27]  Yanbin Li,et al.  Simultaneous detection of Escherichia coli O157:H7 and Salmonella Typhimurium using quantum dots as fluorescence labels. , 2006, The Analyst.

[28]  Zhongpin Zhang,et al.  Shell thickness-dependent Raman enhancement for rapid identification and detection of pesticide residues at fruit peels. , 2012, Analytical chemistry.

[29]  J. Holopainen,et al.  Analytical specificity and sensitivity of a real-time polymerase chain reaction assay for identification of bovine mastitis pathogens. , 2009, Journal of dairy science.

[30]  Mohammed Zourob,et al.  Paper-based magnetic nanoparticle-peptide probe for rapid and quantitative colorimetric detection of Escherichia coli O157:H7. , 2017, Biosensors & bioelectronics.

[31]  Jeremy D. Driskell,et al.  Rapid screening of antibody–antigen binding using dynamic light scattering (DLS) and gold nanoparticles , 2015 .

[32]  V. Ozguz,et al.  Graphene-interfaced electrical biosensor for label-free and sensitive detection of foodborne pathogenic E. coli O157:H7. , 2017, Biosensors & bioelectronics.

[33]  D. Xing,et al.  Sensitive detection of Listeria monocytogenes based on highly efficient enrichment with vancomycin-conjugated brush-like magnetic nano-platforms. , 2017, Biosensors & bioelectronics.

[34]  Olaf Piepenburg,et al.  DNA Detection Using Recombination Proteins , 2006, PLoS biology.

[35]  S. Bamrungsap,et al.  SERS-based immunoassay on 2D-arrays of Au@Ag core–shell nanoparticles: influence of the sizes of the SERS probe and sandwich immunocomplex on the sensitivity , 2017 .

[36]  Haiyang Jiang,et al.  Multiplex Lateral Flow Immunoassays Based on Amorphous Carbon Nanoparticles for Detecting Three Fusarium Mycotoxins in Maize. , 2017, Journal of agricultural and food chemistry.

[37]  Arben Merkoçi,et al.  Magnetic Bead/Gold Nanoparticle Double-Labeled Primers for Electrochemical Detection of Isothermal Amplified Leishmania DNA. , 2016, Small.

[38]  Zhong Lin Wang,et al.  Shell-isolated nanoparticle-enhanced Raman spectroscopy , 2010, Nature.

[39]  C. Elliott,et al.  Development and Validation of a Lateral Flow Immunoassay for the Rapid Screening of Okadaic Acid and All Dinophysis Toxins from Shellfish Extracts. , 2015, Journal of Agricultural and Food Chemistry.