Enhanced second-harmonic-generation detection of collagen by means of optical wavefront shaping

Second-harmonic generation (SHG) has proven to be an effective method to both image and detect structural variations in fibrillar collagen. The ability to detect these differences is especially useful in studying diseases like cancer and fibrosis.1 SHG techniques have historically been limited by their ability to penetrate and image through strongly scattering tissues. Recently, optical wavefront shaping has enabled light to be focused through highly scattering media such as biological tissue.2-4 This technology also enables us to examine the dependence of second harmonic generation on the spatial phase of the pump laser. Here, we demonstrate that wavefront shaping can be used to enhance the generation of second harmonic light from collagen fibrils even when scattering is low or non-existent.

[1]  Mathias Fink,et al.  Time-Reversed Acoustics , 1999 .

[2]  A D McCulloch,et al.  Myocardial Mechanics and Collagen Structure in the Osteogenesis Imperfecta Murine (oim) , 2000, Circulation research.

[3]  Leslie M Loew,et al.  Second-harmonic imaging microscopy for visualizing biomolecular arrays in cells, tissues and organisms , 2003, Nature Biotechnology.

[4]  D. Psaltis,et al.  OPTICAL PHASE CONJUGATION FOR TURBIDITY SUPPRESSION IN BIOLOGICAL SAMPLES. , 2008, Nature photonics.

[5]  Demetri Psaltis,et al.  Digital phase conjugation of second harmonic radiation emitted by nanoparticles in turbid media. , 2010, Optics express.

[6]  O. Katz,et al.  Noninvasive nonlinear focusing and imaging through strongly scattering turbid layers , 2014, 1405.4826.

[7]  A. Mosk,et al.  Focusing coherent light through opaque strongly scattering media. , 2007, Optics letters.

[8]  Demetri Psaltis,et al.  Imaging through turbid layers by scanning the phase conjugated second harmonic radiation from a nanoparticle. , 2010, Optics express.

[9]  A. Mosk,et al.  Exploiting disorder for perfect focusing , 2009, 0910.0873.

[10]  J R Mao,et al.  The Ehlers-Danlos syndrome: on beyond collagens. , 2001, The Journal of clinical investigation.

[11]  Vladislav V. Yakovlev,et al.  Stimulated Brillouin Scattering Microscopic Imaging , 2015, Scientific Reports.

[12]  Monal R. Mehta,et al.  Fourier transform-second-harmonic generation imaging of biological tissues. , 2009, Optics express.

[13]  I. Vellekoop.  Feedback-based wavefront shaping. , 2015, Optics express.

[14]  Sergey Plotnikov,et al.  Second harmonic generation microscopy for quantitative analysis of collagen fibrillar structure , 2012, Nature Protocols.

[15]  Anthony E. Siegman,et al.  Nonlinear-optical calculations using fast-transform methods: Second-harmonic generation with depletion and diffraction , 1980 .

[16]  Marlan O Scully,et al.  Dual Raman-Brillouin Microscope for Chemical and Mechanical Characterization and Imaging. , 2015, Analytical chemistry.

[17]  YongKeun Park,et al.  Recent advances in wavefront shaping techniques for biomedical applications , 2015, 1502.05475.

[18]  B. Hokr,et al.  Optimization of focusing through scattering media using the continuous sequential algorithm , 2016, Journal of modern optics.

[19]  A. Mosk,et al.  Phase control algorithms for focusing light through turbid media , 2007, 0710.3295.