Magnetic Nanosensors for the Detection of Oligonucleotide Sequences.

Superparamagnetic nanoparticles conjugated to oligonucleotides exert sensitive and reversible effects on the spin-spin relaxation rates of adjacent water protons when the conjugates hybridize to target oligonucleotides (see temperature cycle in the picture). The fluid-phase magnetic sensors provide the basis for detecting specific nucleic acid sequences in turbid media and potentially in tissues in vivo.

[1]  C. Mirkin,et al.  Homogeneous, Nanoparticle-Based Quantitative Colorimetric Detection of Oligonucleotides , 2000 .

[2]  Ralph Weissleder,et al.  Tat peptide-derivatized magnetic nanoparticles allow in vivo tracking and recovery of progenitor cells , 2000, Nature Biotechnology.

[3]  Anna Moore,et al.  In vivo magnetic resonance imaging of transgene expression , 2000, Nature Medicine.

[4]  R. Colton,et al.  The BARC biosensor applied to the detection of biological warfare agents. , 2000, Biosensors & bioelectronics.

[5]  F. Kramer,et al.  Thermodynamic basis of the enhanced specificity of structured DNA probes. , 1999, Proceedings of the National Academy of Sciences of the United States of America.

[6]  R Weissleder,et al.  High-efficiency intracellular magnetic labeling with novel superparamagnetic-Tat peptide conjugates. , 1999, Bioconjugate chemistry.

[7]  Chad A. Mirkin,et al.  One-Pot Colorimetric Differentiation of Polynucleotides with Single Base Imperfections Using Gold Nanoparticle Probes , 1998 .

[8]  A. Roda,et al.  Chemiluminescence: a sensitive detection system in in situ hybridization. , 1998, Histology and histopathology.

[9]  T. Meade,et al.  Electron Transfer through DNA: Site‐Specific Modification of Duplex DNA with Ruthenium Donors and Acceptors , 1995 .

[10]  Thomas J. Meade,et al.  Elektronenübertragung in DNA: Ruthenium‐Elektronendonor‐ und ‐acceptorkomplexe als ortsspezifische Modifikationen doppelsträngiger DNA , 1995 .

[11]  R Weissleder,et al.  Monocrystalline iron oxide nanocompounds (MION): Physicochemical properties , 1993, Magnetic resonance in medicine.