Continuous particle separation in a serpentine microchannel via negative and positive dielectrophoretic focusing

Dielectrophoresis (DEP) has been widely used to focus and separate cells and particles in microfluidic devices. This work first demonstrates negative and positive dielectrophoretic focusing of particles in a serpentine microchannel by changing only the electric conductivity of the suspending fluid. Due to the channel turn-induced dielectrophoretic force, particles are focused to either the centerline or the sidewalls of the channel when their electric conductivity is lower (i.e. negative DEP) or higher (i.e. positive DEP) than that of the fluid. These distinctive dielectrophoretic focusing phenomena in a serpentine microchannel are then combined to implement a continuous separation between particles of different sizes and electric conductivities. Such separation eliminates the fabrication of in-channel microelectrodes or micro-insulators that are typically required in DEP-based separation techniques. A numerical model is also developed to predict the particle motion, and the simulation results agree reasonably with the observed particle focusing and separation behaviors.

[1]  A. Singh,et al.  Dielectrophoresis in microchips containing arrays of insulating posts: theoretical and experimental results. , 2003, Analytical chemistry.

[2]  Conrad D. James,et al.  Continuous-mode dielectrophoretic gating for highly efficient separation of analytes in surface micromachined microfluidic devices , 2008 .

[3]  Hywel Morgan,et al.  High throughput particle analysis: combining dielectrophoretic particle focussing with confocal optical detection. , 2006, Biosensors & bioelectronics.

[4]  S. Kalams,et al.  DC-Dielectrophoretic separation of biological cells by size , 2008, Biomedical microdevices.

[5]  J. Voldman.  Electrical forces for microscale cell manipulation. , 2006, Annual review of biomedical engineering.

[6]  H. John Crabtree,et al.  Continuous dielectrophoretic cell separation microfluidic device. , 2007, Lab on a chip.

[7]  Michael P Hughes,et al.  Strategies for dielectrophoretic separation in laboratory‐on‐a‐chip systems , 2002, Electrophoresis.

[8]  Hsueh-Chia Chang,et al.  An integrated dielectrophoretic chip for continuous bioparticle filtering, focusing, sorting, trapping, and detecting. , 2007, Biomicrofluidics.

[9]  Kendra V. Sharp,et al.  Transient simulations of the electrophoretic motion of a cylindrical particle through a 90° corner , 2008 .

[10]  Nicole Pamme,et al.  Continuous flow separations in microfluidic devices. , 2007, Lab on a chip.

[11]  H. Morgan,et al.  The electrokinetic properties of latex particles: comparison of electrophoresis and dielectrophoresis. , 2005, Journal of colloid and interface science.

[12]  E. Cummings,et al.  Dielectrophoretic concentration and separation of live and dead bacteria in an array of insulators. , 2004, Analytical chemistry.

[13]  S. Martínez-Chapa,et al.  Performance characterization of an insulator‐based dielectrophoretic microdevice , 2008, Electrophoresis.

[14]  Junjie Zhu,et al.  Dielectrophoretic focusing of particles in a microchannel constriction using DC‐biased AC flectric fields , 2009, Electrophoresis.

[15]  Tzuen-Rong J Tzeng,et al.  Electrokinetic focusing and filtration of cells in a serpentine microchannel. , 2009, Biomicrofluidics.

[16]  A. B. Frazier,et al.  Lateral-driven continuous dielectrophoretic microseparators for blood cells suspended in a highly conductive medium. , 2008, Lab on a chip.

[17]  Cheng-Hsien Liu,et al.  Dielectrophoresis based‐cell patterning for tissue engineering , 2006, Biotechnology journal.

[18]  Y. Lam,et al.  Dielectrophoretic manipulation of particles in a modified microfluidic H filter with multi-insulating blocks. , 2008, Biomicrofluidics.

[19]  F. Zenhausern,et al.  Electrodeless dielectrophoresis for micro total analysis systems , 2003, IEEE Engineering in Medicine and Biology Magazine.

[20]  M. Hayes,et al.  Electrophoretic and dielectrophoretic field gradient technique for separating bioparticles. , 2007, Analytical chemistry.

[21]  Abraham P Lee,et al.  Dual frequency dielectrophoresis with interdigitated sidewall electrodes for microfluidic flow‐through separation of beads and cells , 2009, Electrophoresis.

[22]  Sungyoung Choi,et al.  Microfluidic system for dielectrophoretic separation based on a trapezoidal electrode array. , 2005, Lab on a chip.

[23]  Saeid Nahavandi,et al.  Dielectrophoretic manipulation and separation of microparticles using curved microelectrodes , 2009, Electrophoresis.

[24]  J.A. Schwartz,et al.  A three-dimensional dielectrophoretic particle focusing channel for microcytometry applications , 2005, Journal of Microelectromechanical Systems.

[25]  Yuejun Kang,et al.  Continuous separation of microparticles by size with Direct current‐dielectrophoresis , 2006, Electrophoresis.

[26]  Chih-Ming Ho,et al.  Cell Separation by Non-Inertial Force Fields in Microfluidic Systems. , 2009, Mechanics research communications.

[27]  S. K. Griffiths,et al.  Conditions for similitude between the fluid velocity and electric field in electroosmotic flow , 1999, Analytical chemistry.

[28]  Thomas Braschler,et al.  Continuous separation of cells by balanced dielectrophoretic forces at multiple frequencies. , 2008, Lab on a chip.

[29]  B. Kirby,et al.  Continuous-flow particle separation by 3D Insulative dielectrophoresis using coherently shaped, dc-biased, ac electric fields. , 2007, Analytical chemistry.

[30]  Yuejun Kang,et al.  Continuous particle separation with localized AC-dielectrophoresis using embedded electrodes and an insulating hurdle , 2009 .

[31]  Mark A Burns,et al.  Electrodeless direct current dielectrophoresis using reconfigurable field‐shaping oil barriers , 2007, Electrophoresis.

[32]  Paul H. Bessette,et al.  Marker-specific sorting of rare cells using dielectrophoresis. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[33]  Marco Rito-Palomares,et al.  Dielectrophoresis for the manipulation of nanobioparticles , 2007, Electrophoresis.

[34]  Young-Ho Cho,et al.  A continuous size-dependent particle separator using a negative dielectrophoretic virtual pillar array. , 2008, Lab on a chip.

[35]  M. Kersaudy-Kerhoas,et al.  Recent advances in microparticle continuous separation. , 2008, IET nanobiotechnology.

[36]  Guoqing Hu,et al.  DC dielectrophoretic focusing of particles in a serpentine microchannel , 2009 .

[37]  Che-Hsin Lin,et al.  Vertical focusing device utilizing dielectrophoretic force and its application on microflow cytometer , 2004 .

[38]  Kwan Hyoung Kang,et al.  Effects of dc-dielectrophoretic force on particle trajectories in microchannels , 2006 .

[39]  E. Cummings,et al.  Insulator‐based dielectrophoresis for the selective concentration and separation of live bacteria in water , 2004, Electrophoresis.

[40]  Klavs F Jensen,et al.  Continuous dielectrophoretic size-based particle sorting. , 2006, Analytical chemistry.

[41]  Peter R. C. Gascoyne,et al.  Dielectrophoresis-based sample handling in general-purpose programmable diagnostic instruments , 2004, Proceedings of the IEEE.

[42]  Murat Okandan,et al.  Monolithic surface micromachined fluidic devices for dielectrophoretic preconcentration and routing of particles , 2006 .

[43]  Xiangchun Xuan,et al.  Particle electrophoresis and dielectrophoresis in curved microchannels. , 2009, Journal of colloid and interface science.

[44]  Shizhi Qian,et al.  DC electrokinetic particle transport in an L-shaped microchannel. , 2010, Langmuir : the ACS journal of surfaces and colloids.