Quinhentos e trinta e tres (n=533) complexos cumulus-oocitos (CCO) bovinos foram utilizados para comparar a suplementacao dos meios de maturacao e cultivo com diferentes fontes proteicas: soro de egua em estro (SEE; n=150) e soro de vaca em estro (SVE; n=123), alcool polivinilico (PVA; n=128) e albumina serica bovina (BSA; n=132). Os grupos de CCO foram distribuidos aletoriamente e maturados in vitro por 24h em TCM199 com 10% de SEE, 10% SVE, 0,1% PVA e 0,6% BSA em estufa de cultivo com 5% de CO2, a 39oC e umidade saturada. A fecundacao foi realizada durante 18h, em meio FERT-TALP em estufa de cultivo. Os embrioes foram cultivados por 8 dias em meio synthetic oviduct fluid (SOFaaci) com 5% SEE, 5% SVE, 0,1% PVA e 0,6% BSA em estufa de cultivo a 39oC, sendo mantidos em bolsas gaseificadas com 5% CO2, 5% O2 e 90% N2. A producao de embrioes no D7 nos tratamentos SEE (33/142; 23%) e SVE (34/103; 33%) foi superior (P In vitro production of bovine embryos in medium supplemented with defined and undefined protein sources Abstract Five hundred and thirty three (n=533) bovine cumulus-oocytes complexes (COC) obtained by aspiration of 2-8mm diameter follicles were used to compare maturation and culture media containing distinct protein sources: estrus mare serum (EMS; n=150), estrus cow serum (ECS=123), polyvinyl alcohol (PVA; n= 128) and bovine serum albumin (BSA; n=132). COC groups were randomly distributed into treatments and in vitro maturated (IVM) in TCM-199 + 10% EMS, 10% ECS, 0.1% PVA and 0.6% BSA for 24h using an incubator with 5% CO2 at 39oC and saturated air humidity. Fertilization was accomplished during 18h in FERT-TALP medium at the same temperature and gas atmosphere of the previous maturation phase. Denudation was performed and the zygotes were cultured for 8 days in synthetic oviduct fluid (SOFaaci) medium + 5% EMS, 5% ECS, 0.1% PVA and 0.6% BSA in an incubator at 39oC using gasified bags with 5% CO2, 5% O2 and 90% N2. Embryo production in D7 for treatments EMS (33/142; 23%) and ECS (34/103; 33%) were higher (P
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