Chemiluminescence lateral flow immunoassay cartridge with integrated amorphous silicon photosensors array for human serum albumin detection in urine samples

AbstractA novel and disposable cartridge for chemiluminescent (CL)-lateral flow immunoassay (LFIA) with integrated amorphous silicon (a-Si:H) photosensors array was developed and applied to quantitatively detect human serum albumin (HSA) in urine samples. The presented analytical method is based on an indirect competitive immunoassay using horseradish peroxidase (HRP) as a tracer, which is detected by adding the luminol/enhancer/hydrogen peroxide CL cocktail. The system comprises an array of a-Si:H photosensors deposited on a glass substrate, on which a PDMS cartridge that houses the LFIA strip and the reagents necessary for the CL immunoassay was optically coupled to obtain an integrated analytical device controlled by a portable read-out electronics. The method is simple and fast with a detection limit of 2.5 mg L−1 for HSA in urine and a dynamic range up to 850 mg L−1, which is suitable for measuring physiological levels of HSA in urine samples and their variation in different diseases (micro- and macroalbuminuria). The use of CL detection allowed accurate and objective analyte quantification in a dynamic range that extends from femtomoles to picomoles. The analytical performances of this integrated device were found to be comparable with those obtained using a charge-coupled device (CCD) as a reference off-chip detector. These results demonstrate that integrating the a-Si:H photosensors array with CL-LFIA technique provides compact, sensitive and low-cost systems for CL-based bioassays with a wide range of applications for in-field and point-of-care bioanalyses. Graphical AbstractA novel integrated portable device was developed for direct quantitative detection of human serum albumin (HSA) in urine samples, exploiting a chemiluminescence lateral flow immunoassay (LFIA). The device comprises a cartridge that holds the LFIA strip and all the reagents necessary for the analysis, an array of amorphous silicon photosensors, and a custom read-out electronics

[1]  Á. Montoya,et al.  Development of a chemiluminescence-based quantitative lateral flow immunoassay for on-field detection of 2,4,6-trinitrotoluene. , 2012, Analytica chimica acta.

[2]  Domenico Caputo,et al.  Multiwell cartridge with integrated array of amorphous silicon photosensors for chemiluminescence detection: development, characterization and comparison with cooled-CCD luminograph , 2014, Analytical and Bioanalytical Chemistry.

[3]  Wenjun Yang,et al.  Near-infrared fluorescence-based multiplex lateral flow immunoassay for the simultaneous detection of four antibiotic residue families in milk. , 2016, Biosensors & bioelectronics.

[4]  Domenico Caputo,et al.  Hydrogenated amorphous silicon ultraviolet sensor for deoxyribonucleic acid analysis , 2006 .

[5]  Domenico Caputo,et al.  Spectral tuned amorphous silicon p-i-n for DNA detection , 2006 .

[6]  J. Lewis,et al.  Microalbuminuria in Type 2 Diabetes and Hypertension , 2008, Diabetes Care.

[7]  Il-Hoon Cho,et al.  Rapid pathogen detection by lateral-flow immunochromatographic assay with gold nanoparticle-assisted enzyme signal amplification. , 2015, International journal of food microbiology.

[8]  Standards of Medical Care for Patients With Diabetes Mellitus , 1991, Diabetes Care.

[9]  Domenico Caputo,et al.  Chemiluminescence-Based Micro-Total-Analysis System with Amorphous Silicon Photodiodes , 2014 .

[10]  Jihea Moon,et al.  Development of a Smartphone-based reading system for lateral flow immunoassay. , 2014, Journal of nanoscience and nanotechnology.

[11]  A. Nascetti,et al.  Microfluidic Chip With Integrated a-Si:H Photodiodes for Chemiluminescence-Based Bioassays , 2013, IEEE Sensors Journal.

[12]  T. Larson,et al.  Quantification of urinary albumin by using protein cleavage and LC-MS/MS. , 2009, Clinical chemistry.

[13]  Shi Lei,et al.  A novel method to detect Listeria monocytogenes via superparamagnetic lateral flow immunoassay , 2015 .

[14]  Aldo Roda,et al.  A multiplex chemiluminescent biosensor for type B-fumonisins and aflatoxin B1 quantitative detection in maize flour. , 2015, The Analyst.

[15]  Domenico Caputo,et al.  Low-temperature admittance measurement in thin film amorphous silicon structures , 1997 .

[16]  A. Roda,et al.  Recent advancements in chemical luminescence-based lab-on-chip and microfluidic platforms for bioanalysis. , 2014, Journal of pharmaceutical and biomedical analysis.

[17]  Fang Zhao,et al.  A novel method to detect Listeria monocytogenes via superparamagnetic lateral flow immunoassay , 2014, Analytical and Bioanalytical Chemistry.

[18]  D. Bruns,et al.  [Current issues in measurement and reporting of urinary albumin excretion]. , 2010, Annales de biologie clinique.

[19]  Aldo Roda,et al.  A simple and compact smartphone accessory for quantitative chemiluminescence-based lateral flow immunoassay for salivary cortisol detection. , 2015, Biosensors & bioelectronics.

[20]  João Pedro Conde,et al.  Microspot-based ELISA in microfluidics: chemiluminescence and colorimetry detection using integrated thin-film hydrogenated amorphous silicon photodiodes. , 2011, Lab on a chip.

[21]  V. Chu,et al.  Detection of ochratoxin A in wine and beer by chemiluminescence-based ELISA in microfluidics with integrated photodiodes , 2013 .

[22]  Michael Eberhard,et al.  Handheld and Portable Reader Devices for Lateral Flow Immunoassays , 2009 .

[23]  C. Baggiani,et al.  A fluorescent immunochromatographic strip test using Quantum Dots for fumonisins detection. , 2016, Talanta.

[24]  Laura Anfossi,et al.  Development and application of a quantitative lateral flow immunoassay for fumonisins in maize. , 2010, Analytica chimica acta.

[25]  Domenico Caputo,et al.  Amorphous silicon photosensors integrated in microfluidic structures as a technological demonstrator of a “true” Lab-on-Chip system , 2015 .

[26]  Jerome P. Smith,et al.  Enhanced Performance of Methamphetamine Lateral Flow Cassettes Using an Electronic Lateral Flow Reader , 2015, Journal of occupational and environmental hygiene.

[27]  Rong-Liang Liang,et al.  Rapid and sensitive lateral flow immunoassay method for determining alpha fetoprotein in serum using europium (III) chelate microparticles-based lateral flow test strips. , 2015, Analytica chimica acta.

[28]  A. Roda,et al.  Chemiluminescence-based biosensor for fumonisins quantitative detection in maize samples. , 2012, Biosensors & bioelectronics.

[29]  C. Mogensen,et al.  The Stages in Diabetic Renal Disease: With Emphasis on the Stage of Incipient Diabetic Nephropathy , 1983, Diabetes.

[30]  F. Vella.  Textbook of clinical chemistry: Edited by N W Tietz. Pp 1919. W B Saunders, Philadelphia. 1986 ISBN 0‐7216‐8886‐1 , 1986 .

[31]  H. Adachi.  Microalbuminuria is an independent prognostic information for cardiovascular disease. , 2014, Atherosclerosis.

[32]  Michael C. Brown,et al.  Antibodies: Key to a Robust Lateral Flow Immunoassay , 2009 .

[33]  A. Nascetti,et al.  Amorphous Silicon Photosensors for Detection of Ochratoxin a in Wine , 2012, IEEE Sensors Journal.

[34]  Raphael C. Wong,et al.  Lateral flow immunoassay , 2009 .

[35]  Leszek Czupryniak,et al.  Nowy rok, stare problemy, ale i nowe możliwości , 2014 .

[36]  N. Pires,et al.  Measurement of salivary cortisol by a chemiluminescent organic-based immunosensor. , 2014, Bio-medical materials and engineering.

[37]  C. Burtis.  Tietz textbook of Clinical Chemistry , 1994 .

[38]  V. Rai,et al.  Electrochemical lateral flow immunosensor for detection and quantification of dengue NS1 protein. , 2016, Biosensors & bioelectronics.

[39]  R. de la Torre,et al.  Capillary gas chromatography-mass spectrometry quantitative determination of hydroxytyrosol and tyrosol in human urine after olive oil intake. , 2001, Analytical biochemistry.

[40]  D. Caputo,et al.  Microfluidic cartridge with integrated array of amorphous silicon photosensors for chemiluminescence detection of viral DNA , 2016 .

[41]  Reinhard Niessner,et al.  Chemiluminescence microarrays in analytical chemistry: a critical review , 2014, Analytical and Bioanalytical Chemistry.

[42]  J. Conrad,et al.  Aptamer-Phage Reporters for Ultrasensitive Lateral Flow Assays. , 2015, Analytical chemistry.

[43]  Brendan O’Farrell,et al.  Evolution in Lateral Flow–Based Immunoassay Systems , 2008, Lateral Flow Immunoassay.