IDENTIFICATION AND ANALYSIS OF WHITE SPOT SYNDROME VIRUS (WSSV) ENVELOPE PROTEIN VP41A

White spot syndrome virus (WSSV) is the causative agent of a disease that has led to severe mortalities of cultured shrimp in Taiwan and many other countries. WSSV, a kind of large enveloped DNA virus, has a wide host range among crustaceans. During the first stage to infect a target cell, the enveloped virus must combine itself with the receptor on the host cell membrane via an envelope protein. In WSSV, binding of envelope protein with the receptor of the host cell membrane was revealed in a number of previous studies. WSSV envelope proteins were also considered to form a protein complex, at some stage in all research to be named “infectome”. The WSSV envelope protein VP41A, with the predicted molecular weight of about 41 kDa and having a transmembrane region at its C-terminus, is identified in this study. The interaction of VP41A with the virus’ major envelope protein VP26 is revealed by His pull-down and liquid chromatography tandem mass spectrometry. Furthermore, the protein-protein interaction of VP41A with VP28 and VP56 is verified by far western blotting. This data indicate that VP41A participates in the formation of a WSSV protein complex that may be involved in WSSV infection. These findings may identify certain areas for further WSSV research.

[1]  Feng Yang,et al.  Proteomic Analysis of the Major Envelope and Nucleocapsid Proteins of White Spot Syndrome Virus , 2006, Journal of Virology.

[2]  J. Chen,et al.  Characterization of a novel envelope protein WSV010 of shrimp white spot syndrome virus and its interaction with a major viral structural protein VP24. , 2007, Virology.

[3]  C. Lo,et al.  Penaeus monodon chitin-binding protein (PmCBP) is involved in white spot syndrome virus (WSSV) infection. , 2009, Fish & shellfish immunology.

[4]  C. Lo,et al.  PmRab7 Is a VP28-Binding Protein Involved in White Spot Syndrome Virus Infection inShrimp , 2006, Journal of Virology.

[5]  H. Nauwynck,et al.  A review on the morphology, molecular characterization, morphogenesis and pathogenesis of white spot syndrome virus. , 2007, Journal of fish diseases.

[6]  C. Lo,et al.  Characterization of White Spot Syndrome Virus Envelope Protein VP51A and Its Interaction with Viral Tegument Protein VP26 , 2008, Journal of Virology.

[7]  C. Lo,et al.  A 3D Model of the Membrane Protein Complex Formed by the White Spot Syndrome Virus Structural Proteins , 2010, PloS one.

[8]  Q. Wan,et al.  VP26 of White Spot Syndrome Virus Functions as a Linker Protein between the Envelope and Nucleocapsid of Virions by Binding with VP51 , 2008, Journal of Virology.

[9]  Jie Huang,et al.  White spot syndrome virus VP37 interacts with VP28 and VP26. , 2009, Diseases of aquatic organisms.

[10]  Xixian Xie,et al.  White spot syndrome virus VP24 interacts with VP28 and is involved in virus infection. , 2006, The Journal of general virology.

[11]  S. White,et al.  RNAi-mediated chromatin silencing in fission yeast. , 2008, Current topics in microbiology and immunology.

[12]  Xiaobo Zhang,et al.  Identification of structural proteins from shrimp white spot syndrome virus (WSSV) by 2DE‐MS , 2004, Proteins.

[13]  Hai-Jie Yang,et al.  β-integrin mediates WSSV infection , 2007 .

[14]  Feng Yang,et al.  The C-terminal region of envelope protein VP38 from white spot syndrome virus is indispensable for interaction with VP24 , 2008, Archives of Virology.

[15]  Jyh-Ming Tsai,et al.  Genomic and Proteomic Analysis of Thirty-Nine Structural Proteins of Shrimp White Spot Syndrome Virus , 2004, Journal of Virology.

[16]  C. Lo,et al.  Detection of baculovirus associated with white spot syndrome (WSBV) in penaeid shrimps using polymerase chain reaction , 1996 .

[17]  C. Lo,et al.  White spot syndrome virus envelope protein VP53A interacts with Penaeus monodon chitin-binding protein (PmCBP). , 2007, Diseases of aquatic organisms.

[18]  J. E. Vickers,et al.  A non-occluded, systemic baculovirus that occurs in cells of ectodermal and mesodermal origin and causes high mortality in the black tiger prawn Penaeus monodon. , 1995 .

[19]  Chen-Fang Chang,et al.  Purification and genomic analysis of baculovirus associated with white spot syndrome (WSBV) of Penaeus monodon , 1995 .

[20]  Feng Yang,et al.  Identification and characterization of a prawn white spot syndrome virus gene that encodes an envelope protein VP31. , 2005, Virology.

[21]  Feng Yang,et al.  The RGD motif in VP31 of white spot syndrome virus is involved in cell adhesion , 2011, Archives of Virology.

[22]  J. Vlak,et al.  Protection of Penaeus monodon against white spot syndrome virus using a WSSV subunit vaccine. , 2004, Fish & shellfish immunology.

[23]  Hongyan Li,et al.  A simple and efficient method for purification of intact white spot syndrome virus (WSSV) viral particles. , 2005, Virus research.

[24]  Y. Qi,et al.  Four Major Envelope Proteins of White Spot Syndrome Virus Bind To Form a Complex , 2009, Journal of Virology.

[25]  C. Lo,et al.  Identification of the Nucleocapsid, Tegument, and Envelope Proteins of the Shrimp White Spot Syndrome Virus Virion , 2006, Journal of Virology.