Depth-resolved fluorescence spectroscopy of normal and dysplastic cervical tissue.

A portable confocal system with the excitations at 355nm and 457nm was instrumented to investigate the depth-resolved fluorescence of cervical tissue. The study focused on extracting biochemical and morphological information carried in the depth-resolved signals measured from the normal squamous epithelial tissue and squamous intraepithelial lesions. Strong keratin fluorescence with the spectral characteristics similar to collagen were observed from the topmost keratinizing layer of all tissue samples. It was found that NADH and FAD fluorescence measured from the underlying non-keratinizing epithelial layer were strongly correlated to the tissue pathology. This study demonstrates that the depth-resolved fluorescence spectroscopy can potentially provide more accurate diagnostic information for determining tissue pathology.

[1]  R. Richards-Kortum,et al.  Microanatomical and Biochemical Origins of Normal and Precancerous Cervical Autofluorescence Using Laser-scanning Fluorescence Confocal Microscopy¶ , 2003, Photochemistry and photobiology.

[2]  Peng Xi,et al.  Depth-resolved fluorescence spectroscopy reveals layered structure of tissue. , 2004, Optics express.

[3]  Nirmala Ramanujam,et al.  Optimal methods for fluorescence and diffuse reflectance measurements of tissue biopsy samples , 2002, Lasers in surgery and medicine.

[4]  Rebecca R. Richards-Kortum,et al.  Optimal excitation wavelengths for discrimination of cervical neoplasia , 2002, IEEE Transactions on Biomedical Engineering.

[5]  Michele Follen,et al.  Autofluorescence Microscopy of Fresh Cervical‐Tissue Sections Reveals Alterations in Tissue Biochemistry with Dysplasia ¶ , 2001 .

[6]  S. Shapshay,et al.  Spectroscopic detection and evaluation of morphologic and biochemical changes in early human oral carcinoma , 2003, Cancer.

[7]  Rebecca Richards-Kortum,et al.  Determination of epithelial tissue scattering coefficient using confocal microscopy , 2003 .

[8]  N Ramanujam,et al.  In vivo diagnosis of cervical intraepithelial neoplasia using 337-nm-excited laser-induced fluorescence. , 1994, Proceedings of the National Academy of Sciences of the United States of America.

[9]  B. Chance,et al.  Oxidation-reduction ratio studies of mitochondria in freeze-trapped samples. NADH and flavoprotein fluorescence signals. , 1979, The Journal of biological chemistry.

[10]  L. David,et al.  Keratins 8, 10, 13, and 17 are useful markers in the diagnosis of human cervix carcinomas. , 2004, Human pathology.