Detection and quantitation by fluorescence in situ hybridization (FISH) and image analysis of HER‐2/neu gene amplification in breast cancer fine‐needle samples

Fine‐needle sampling, although a practical and noninvasive method of tissue acquisition, has rarely been used for HER‐2/neu fluorescent in situ hybridization (FISH). To assess HER‐2/neu gene amplification in mammary carcinoma, FISH signals on cytology and corresponding tissue biopsies were detected visually and measured by image analysis. The results were correlated with patient and tumor characteristics.

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