Electrochemically deposited nanocomposite of chitosan and carbon nanotubes for detection of human chorionic gonadotrophin.

A new label-free amperometric immunosensor was developed for detection of human chorionic gonadotrophin (hCG) based on multiwall carbon nanotubes-chitosan (MWNTs-CS) complex film and three-dimensional AuNPs-TiO(2) hybrid. Firstly, MWNTs-CS film was deposited on a glassy carbon electrode (GCE) by a simple and controllable electrodeposition method. Next, thionine (Thi), as a redox probe, was covalently bound onto the MWNTs-CS film with glutaraldehyde (GA) to obtain the Thi/MWNTs-CS film. The free amino groups of the composite membrane were used to adsorb AuNPs-TiO(2) for immobilizing human chorionic gonadotrophin antibody (anti-hCG) because of its large surface area and satisfactory biocompatibility. At last, BSA was employed to block possible remaining active sites. Under optimized conditions, the immunosensor displayed high sensitivity, good reproducibility, and a low detection limit of 0.08mIU/mL at 3 times the background noise. The ease of non-manual technique and the promising feature of biocomposite could serve as a versatile platform for constructing other biosensors.

[1]  Maogen Zhang,et al.  Carbon nanotube-chitosan system for electrochemical sensing based on dehydrogenase enzymes. , 2004, Analytical chemistry.

[2]  Masato Saito,et al.  Nanomaterial-based electrochemical biosensors for medical applications , 2008 .

[3]  D. Wong,et al.  Comparative study of thiolated protein G scaffolds and signal antibody conjugates in the development of electrochemical immunosensors. , 2007, Biosensors & bioelectronics.

[4]  Y. Chai,et al.  Dendritic Silver/Silicon Dioxide Nanocomposite Modified Electrodes for Electrochemical Sensing of Hydrogen Peroxide , 2008 .

[5]  Xiangwei Zhao,et al.  Three-dimensionally ordered macroporous (3DOM) gold-nanoparticle-doped titanium dioxide (GTD) photonic crystals modified electrodes for hydrogen peroxide biosensor. , 2009, Biosensors & bioelectronics.

[6]  Lei Wang,et al.  Flexible carbon nanotube-polymer composite films with high conductivity and superhydrophobicity made by solution process. , 2008, Nano letters.

[7]  Dermot Diamond,et al.  Development of a biosensor for endocrine disrupting compounds based on tyrosinase entrapped within a poly(thionine) film. , 2004, Biosensors & bioelectronics.

[8]  Feng Yan,et al.  A gold nanoparticles/sol-gel composite architecture for encapsulation of immunoconjugate for reagentless electrochemical immunoassay. , 2006, Biomaterials.

[9]  Fan Yu,et al.  A novel piezoelectric quartz micro-array immunosensor based on self-assembled monolayer for determination of human chorionic gonadotropin. , 2004, Biosensors & bioelectronics.

[10]  Jing‐Juan Xu,et al.  Electrochemically deposited nanocomposite of chitosan and carbon nanotubes for biosensor application. , 2005, Chemical communications.

[11]  Joseph Wang,et al.  Adaptive Nanowires for On-Demand Control of Electrochemical Microsystems , 2008 .

[12]  A F Chetcuti,et al.  An indirect perfluorosulfonated ionomer-coated electrochemical immunosensor for the detection of the protein human chorionic gonadotrophin. , 1999, Analytical chemistry.

[13]  G. Frens.  Controlled Nucleation for the Regulation of the Particle Size in Monodisperse Gold Suspensions , 1973 .

[14]  Song Zhang,et al.  Protein chips and nanomaterials for application in tumor marker immunoassays. , 2009, Biosensors & bioelectronics.

[15]  Na Li,et al.  New Antibody Immobilization Strategy Based on Gold Nanoparticles and Azure I/Multi-Walled Carbon Nanotube Composite Membranes for an Amperometric Enzyme Immunosensor , 2007 .

[16]  J. Turkevich,et al.  Coagulation of Colloidal Gold , 2002 .

[17]  C. M. Li,et al.  Direct electrochemistry of hemoglobin on carbonized titania nanotubes and its application in a sensitive reagentless hydrogen peroxide biosensor. , 2008, Biosensors & bioelectronics.

[18]  Kaiming Ye,et al.  Development of Immunosensors Using Carbon Nanotubes , 2008, Biotechnology progress.

[19]  Hua Yang,et al.  The preparation of reagentless electrochemical immunosensor based on a nano-gold and chitosan hybrid film for human chorionic gonadotrophin. , 2009, Analytica chimica acta.

[20]  H. Ju,et al.  Gold Nanoparticles Doped Three-Dimensional Sol-gel Matrix for Amperometric Human Chorionic Gonadotrophin Immunosensor , 2006 .

[21]  Joseph Wang,et al.  Potentiometric immunoassay with quantum dot labels. , 2007, Analytical chemistry.

[22]  A. Domb,et al.  Chitosan chemistry and pharmaceutical perspectives. , 2004, Chemical reviews.

[23]  Hyunmin Yi,et al.  Electrochemically Induced Deposition of a Polysaccharide Hydrogel onto a Patterned Surface , 2003 .

[24]  Zhiliang Jiang,et al.  Immunonanogold-catalytic resonance scattering spectral assay of trace human chorionic gonadotrophin. , 2008, Talanta.

[25]  G. Frens.  Controlled nucleation for the regulation of the particle size in monodisperse gold solutions , 1973 .

[26]  S. Birken,et al.  Immunochemical measurement of early pregnancy isoforms of HCG: potential applications to fertility research, prenatal diagnosis, and cancer. , 2001, Archives of medical research.

[27]  Joseph Wang,et al.  Ultrasensitive electrical biosensing of proteins and DNA: carbon-nanotube derived amplification of the recognition and transduction events. , 2004, Journal of the American Chemical Society.

[28]  Jing-Juan Xu,et al.  A glucose biosensor based on chitosan-glucose oxidase-gold nanoparticles biocomposite formed by one-step electrodeposition. , 2004, Analytical biochemistry.

[29]  Michael J. Tarlov,et al.  Electrochemical study of chitosan films deposited from solution at reducing potentials , 2006 .

[30]  M. Moskovits,et al.  Imaging 3-aminopropyltriethoxysilane self-assembled monolayers on nanostructured titania and tin (IV) oxide nanowires using colloidal silver nanoparticles , 2009 .

[31]  Xianfu Lin,et al.  One-step construction of biosensor based on chitosan-ionic liquid-horseradish peroxidase biocomposite formed by electrodeposition. , 2008, Biosensors & bioelectronics.

[32]  Minghui Yang,et al.  Layer-by-layer self-assembled multilayer films of carbon nanotubes and platinum nanoparticles with polyelectrolyte for the fabrication of biosensors. , 2006, Biomaterials.