Determination of patulin in Chinese herbs by UHPLC- triple quadrupole MS with dispersive solid phase extraction

Objective To establish a UHPLC- MS /MS method for determination of patulin in Chinese herbs. Methods After pectinase enzymolysis and extraction by acetonitrile water solution,the sample extraction solution were conducted dispersive solid phase extraction by mix powder of MgSO 4 and NaAC( 4∶ 1),then purified on Mycrosep228 AlaPat column,finally analyzed by UHPLC- triple quadrupole MS. Results Patulin showed good linear relationship with ion current peak area within the range of 5 ng / ml ~ 250 ng / ml,and the linear equation was Y = 5. 37 × 103X + 151,r = 0. 9998. The recovery was between 71% ~ 99%. The LODs in herbs were in the range of 0. 3 μg / kg ~ 0. 5 μg / kg. Conclusion The method is sensitive,rapid,accurate and specific enough to determine patulin in Chinese herbs.