An association between the factor VII coagulant activity and thrombin activity induced by surface/cold exposure of normal human plasma

Summary. To test whether factor VII activation correlated with the generation of thrombin activity when plasma was exposed to a glass surface and reduced temperature, an association was sought between the changes in factor VII clotting activity (VIIc) and fibrinopeptide A concentration (an index of thrombin activity) in platelet‐poor citrated plasma from 42 healthy adults. The Spearman rank correlation (rs) between responses was 0.82 (P < 0.001). The VIIc assay response to surface/cold exposure was unaffected when thrombin was suppressed by hirudin. An assay for factor VII activity based upon its activation of tritiated factor X revealed an association between the increase in fibrinopeptide A concentration and reduction in functional factor VII concentration during activation of a subset of 22 plasma samples (rs= ‐0.62; (P < 0.003). This loss of functional factor VII was probably due to conversion of active factor VII to its non‐functional end‐product by factor Xa. The results suggest that VIIc is an index of flux within the coagulation system and support the hypothesis that a high VIIc is an indicator of a hypercoagulable state.

[1]  W. Kannel,et al.  Serum cholesterol, lipoproteins, and the risk of coronary heart disease. The Framingham study. , 2020, Annals of internal medicine.

[2]  S. Thompson,et al.  Assay of factor VII activity by two techniques: evidence for increased conversion of VII to αVIIa in hyperlipidaemia, with possible implications for ischaemic heart disease , 1985, British journal of haematology.

[3]  C. Haanen,et al.  Separation of human bone marrow by counterflow centrifugation monitored by DNA‐flowcytometry , 1984, British journal of haematology.

[4]  T. P. Welch,et al.  FIBRINOPEPTIDE A AND SUDDEN CORONARY DEATH , 1984, The Lancet.

[5]  J. Oberdick,et al.  The dual role of factor VII in blood coagulation. Initiation and inhibition of a proteolytic system by a zymogen. , 1982, The Journal of biological chemistry.

[6]  S. Rapaport,et al.  Isolation and characterization of human factor VII. Activation of factor VII by factor Xa. , 1981, The Journal of biological chemistry.

[7]  S. Thompson,et al.  HÆMOSTATIC FUNCTION AND CARDIOVASCULAR DEATH: EARLY RESULTS OF A PROSPECTIVE STUDY , 1980, The Lancet.

[8]  J. Griffin,et al.  Evidence for the participation of both activated factor XII and activated factor IX in cold-promoted activation of factor VII. , 1978, Thrombosis research.

[9]  Y. Nemerson,et al.  Kinetics of the activation of bovine coagulation factor X by components of the extrinsic pathway. Kinetic behavior of two-chain factor VII in the presence and absence of tissue factor. , 1977, The Journal of biological chemistry.

[10]  V. P. Butler,et al.  The generation of fibrinopeptide A in clinical blood samples: evidence for thrombin activity. , 1976, The Journal of clinical investigation.

[11]  O. Ratnoff,et al.  Alteration of factor VII activity by activated Fletcher factor (a plasma kallikrein): a potential link between the intrinsic and extrinsic blood-clotting systems. , 1975, The Journal of laboratory and clinical medicine.

[12]  Y. Nemerson,et al.  Activation and control of factor VII by activated factor X and thrombin. Isolation and characterization of a single chain form of factor VII. , 1975, The Journal of biological chemistry.

[13]  T. Meade,et al.  Factor VII in an Industrial Population , 1974, British journal of haematology.

[14]  H. Gjønnaess.  Cold promoted activation of factor VII. I. Evidence for the existence of an activator. , 1972, Thrombosis et diathesis haemorrhagica.

[15]  L. Carlson,et al.  Ischaemic heart-disease in relation to fasting values of plasma triglycerides and cholesterol. Stockholm prospective study. , 1972, Lancet.

[16]  G. Ashwell,et al.  Studies on the chemical and enzymatic modification of glycoproteins. A general method for the tritiation of sialic acid-containing glycoproteins. , 1971, The Journal of biological chemistry.

[17]  O D RATNOFF,et al.  Role of Hageman factor in the initiation of clotting by glass; evidence that glass frees Hageman factor from inhibition. , 1958, The American journal of medicine.

[18]  S. Thompson,et al.  Haemostatic function and cardiovascular death: early results of a prospective study. , 1980, Lancet.

[19]  O. Ratnoff,et al.  Surface-mediated reactions. , 1979, Current topics in hematology.

[20]  R. Colman,et al.  Activation of the kallikrein system in hyperbetalipoproteinemia. , 1978, The Journal of laboratory and clinical medicine.