Comparative serology of porcine reproductive and respiratory syndrome in eight European laboratories, using immunoperoxidase monolayer assay and enzyme-linked immunosorbent assay.

The members of a European Community Concerted Action participated in a study to compare in-house and commercial tests for the serodiagnosis of porcine reproductive and respiratory syndrome (PRRS). These tests included the immunoperoxidase monolayer assay (IPMA) and enzyme-linked immunosorbent assay (ELISA). The trials involved assays on experimentally-produced reference sera, a blind trial of sera of known status, and a comparative study of negative and 'problem' field sera. The results showed a high level of agreement among IPMA tests used in the participating laboratories at the herd level, with only minor inconsistencies in testing early post-infection sera. A blocking ELISA, used in experimental work by one laboratory, was almost as sensitive as IPMA in assays of reference and validation trial sera. Commercial ELISAs were generally less sensitive than IPMA when used to assay ten-day post-infection sera. Discrepant results among certain field sera are likely to be due to antigenic variation among PRRS viruses in Europe. This may lead to increasing instances of misdiagnosis using tests which rely solely on test antigens derived from single PRRS virus isolates.

[1]  D. Paton,et al.  Production, characterization and reactivity of monoclonal antibodies to porcine reproductive and respiratory syndrome virus. , 1995, The Journal of general virology.

[2]  M. Pensaert,et al.  Comparative study of a blocking enzyme-linked immunosorbent assay and the immunoperoxidase monolayer assay for the detection of antibodies to the porcine reproductive and respiratory syndrome virus in pigs , 1995, Journal of Virological Methods.

[3]  S. Goyal,et al.  A Modified Serum Neutralization Test for the Detection of Antibody to Porcine Reproductive and Respiratory Syndrome Virus in Swine Sera , 1994, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[4]  J. Christopher-Hennings,et al.  Differentiation of U.S. and European isolates of porcine reproductive and respiratory syndrome virus by monoclonal antibodies , 1993, Journal of clinical microbiology.

[5]  R. Morrison,et al.  An Indirect Fluorescent Antibody Test for the Detection of Antibody to Swine Infertility and Respiratory Syndrome Virus in Swine Sera , 1992, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[6]  A. den Besten,et al.  Antigenic Comparison of Lelystad Virus and Swine Infertility and Respiratory Syndrome (SIRS) Virus , 1992, Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc.

[7]  P. Vannier,et al.  An enzyme linked immunosorbent assay (ELISA) for the detection of antibodies to the porcine reproductive and respiratory syndrome (PRRS) virus. , 1992, Annales de recherches veterinaires. Annals of veterinary research.

[8]  P. Plagemann,et al.  Immune complexes that bind to ELISA plates not coated with antigen in mice infected with lactate dehydrogenase-elevating virus: relationship to IgG2a- and IgG2b-specific polyclonal activation of B cells. , 1992, Viral immunology.

[9]  M. Voets,et al.  Mystery swine disease in The Netherlands: the isolation of Lelystad virus. , 1991, The Veterinary quarterly.

[10]  K. Keffaber Reproductive failure of unknown etiol-ogy. , 1989 .