The immunocytochemical localization of glutamate in the electrosensory system of the gymnotiform fish,Apteronotus leptorhynchus

The first processing station of the electrosensory system is the medullary electrosensory lateral line lobe. The electrosensory lateral line lobe of gymnotiform fish is a laminated structure with three anatomically distinct excitatory inputs: primary afferents from electroreceptors, the direct feedback from n. praeminentialis and indirect feedback from n. praeminentialis via a cerebellar lobe--the eminentia granularis posterior. We utilized immunocytochemistry on L-R White-embedded semithin sections, and immunogold on thin sections to localize glutamate and aspartate in the electrosensory lateral line lobe, its afferent inputs and efferent targets. The excitatory inputs and the electrosensory lateral line lobe projection neurons are immunopositive for glutamate only, suggesting that glutamate rather than aspartate is the excitatory transmitter of the electrosensory system.

[1]  L Maler,et al.  The nucleus praeeminentialis: A Golgi study of a feedback center in the electrosensory system of gymnotid fish , 1983, The Journal of comparative neurology.

[2]  J. Bancroft,et al.  Theory and Practice of Histological Techniques , 1990 .

[3]  L. Maler,et al.  The cytology of the posterior lateral line lobe of high‐frequency weakly electric fish (gymnotidae): Dendritic differentiation and synaptic specificity in a simple cortex , 1981, The Journal of comparative neurology.

[4]  C. Carr,et al.  A time-comparison circuit in the electric fish midbrain. I. Behavior and physiology , 1986, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[5]  W Heiligenberg,et al.  Ultrastructural studies of physiologically identified electrosensory afferent synapses in the gymnotiform fish, Eigenmannia , 1987, The Journal of comparative neurology.

[6]  J. Bastian.  Electrolocation: II. The effects of moving objects and other electrical stimuli on the activities of two categories of posterior lateral line lobe cells inApteronotus albifrons , 1981 .

[7]  C. Cotman,et al.  Evaluation of glutamate as a neurotransmitter of cerebellar parallel fibers , 1978, Neuroscience.

[8]  C. Carr,et al.  Peripheral organization and central projections of the electrosensory nerves in gymnotiform fish , 1982, The Journal of comparative neurology.

[9]  W Heiligenberg,et al.  Electrosensory systems in fish , 1990, Synapse.

[10]  L. Maler,et al.  The laminar distribution of amino acids in the caudal cerebellum and electrosensory lateral line lobe of weakly electric fish (Gymnotidae) , 1987, Brain Research.

[11]  C E Carr,et al.  A time-comparison circuit in the electric fish midbrain. II. Functional morphology , 1986, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[12]  O. P. Ottersen,et al.  Metabolic compartmentation of glutamate and glutamine: Morphological evidence obtained by quantitative immunocytochemistry in rat cerebellum , 1992, Neuroscience.

[13]  L. Maler,et al.  Correlating gamma‐aminobutyric acidergic circuits and sensory function in the electrosensory lateral line lobe of a gymnotiform fish , 1994, The Journal of comparative neurology.

[14]  Theodore H. Bullock,et al.  Significance of Findings on Electroreception for General Neurobiology , 1993 .

[15]  C E Carr,et al.  Efferent projections of the posterior lateral line lobe in gymnotiform fish , 1982, The Journal of comparative neurology.

[16]  P. Somogyi,et al.  Quantification of immunogold labelling reveals enrichment of glutamate in mossy and parallel fibre terminals in cat cerebellum , 1986, Neuroscience.

[17]  H. Karten,et al.  Differential projections of ordinary lateral line receptors and electroreceptors in the gymnotid fish, Apteronotus (Sternarchus) albifrons , 1974, The Journal of comparative neurology.

[18]  Joseph Bastian,et al.  Descending control of electroreception. I. Properties of nucleus praeeminentialis neurons projecting indirectly to the electrosensory lateral line lobe , 1990, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[19]  J. Bastian.  Receptive fields of cerebellar cells receiving exteroceptive input in a Gymnotid fish. , 1975, Journal of neurophysiology.

[20]  D. Monaghan,et al.  The distribution of excitatory amino acid binding sites in the brain of an electric fish, Apteronotus leptorhynchus , 1991, Journal of Chemical Neuroanatomy.

[21]  C E Carr,et al.  Laminar organization of the afferent and efferent systems of the torus semicircularis of Gymnotiform fish: Morphological substrates for parallel processing in the electrosensory system , 1981, The Journal of comparative neurology.

[22]  J. Bastian.  Electrolocation: I. How the electroreceptors ofApteronotus albifrons code for moving objects and other electrical stimuli , 1981 .

[23]  F. Orrego,et al.  The chemical nature of the main central excitatory transmitter: A critical appraisal based upon release studies and synaptic vesicle localization , 1993, Neuroscience.

[24]  L. Maler,et al.  The posterior lateral line lobe of certain gymnotoid fish: Quantitative light microscopy , 1979, The Journal of comparative neurology.

[25]  R K Carder,et al.  Neuronal characterization, compartmental distribution, and activity- dependent regulation of glutamate immunoreactivity in adult monkey striate cortex , 1994, The Journal of neuroscience : the official journal of the Society for Neuroscience.

[26]  F. Conti,et al.  Characterization of antisera to glutamate and aspartate. , 1988, The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society.