Bioremediation of trichloroethylene and cis-1,2-dichloroethylene-contaminated groundwater by methane-utilizing bacteria.

Experimental studies on the bioremediation of groundwater contaminated with low concentration trichloroethylene (TCE) and cis1,2-dichloroethylene (DCE) were performed with two sets of bioreactors. Reactors No. 1 and No. 2 were operated without and with methane supplement, respectively. No inoculum was used. The concentrations of TCE and DCE in the effluent and the off gas from reactor No. 2 were much lower than those from reactor No. 1. When air and an H2O2 solution were supplied to reactor No. 2, concentrations of TCE and DCE in the effluent and the off gas were lower than the lowest detectable limit. The population of methane-utilizing bacteria in reactor No. 2 was 1,000 times higher than that in groundwater or in the effluent from reactor No. 1. These methane-utilizing bacteria were apparently attributable to the treatment of TCE.

[1]  K. Arai,et al.  Some characteristics of bacteria found in a bioreactor to treat trichloroethylene-contaminated groundwater. , 1999, Journal of Veterinary Medical Science.

[2]  W. Mcelhenney,et al.  Drinking water contaminants (arsenic, cadmium, lead, benzene, and trichloroethylene). 1. Interaction of contaminants with nutritional status on general performance and immune function in broiler chickens. , 1997, Poultry science.

[3]  W. Mcelhenney,et al.  Drinking water contaminants (arsenic, cadmium, lead, benzene, and trichloroethylene). 2. Effects on reproductive performance, egg quality, and embryo toxicity in broiler breeders. , 1997, Poultry science.

[4]  D. Fennell,et al.  Methanotrophic attached‐film reactor development and biofilm characteristics , 1992, Biotechnology and bioengineering.

[5]  L. Semprini,et al.  Pilot scale field studies of in situ bioremediation of chlorinated solvents , 1992 .

[6]  Masahiro Takahashi,et al.  Effects of biomass entrapment and carrier properties on the performance of an air-fluidized-bed biofilm reactor , 1992 .

[7]  G. Stucki,et al.  Biological degradation of 1,2-dichloroethane under groundwater conditions , 1992 .

[8]  M. Mcfarland,et al.  Methanotrophic cometabolism of trichloroethylene (TCE) in a two stage bioreactor system , 1992 .

[9]  A. Farkas,et al.  ES&T Regulatory Focus: Commercializing innovative cleanup technologies , 1992 .

[10]  B. Fox,et al.  Haloalkene oxidation by the soluble methane monooxygenase from Methylosinus trichosporium OB3b: mechanistic and environmental implications. , 1990, Biochemistry.

[11]  A. T. Moore,et al.  Biodegradation of trans-1,2-dichloroethylene by methane-utilizing bacteria in an aquifer simulator , 1989 .

[12]  P. Palumbo.  Statistics and the practicing physician. , 1988, Mayo Clinic proceedings.

[13]  M. E. Desmond,et al.  The effects of low dosages of trichloroethylene on chick development. , 1983, Toxicology.

[14]  R. Whittenbury,et al.  Enrichment, isolation and some properties of methane-utilizing bacteria. , 1970, Journal of general microbiology.

[15]  E. Kokufuta,et al.  Trichloroethylene degradation by cells of a methane-utilizing bacterium, Methylocystis sp. M, immobilized in calcium alginate , 1995 .

[16]  T. Phelps,et al.  Biodegradation of chlorinated aliphatic hydrocarbon mixtures in a single-pass packed-bed reactor , 1993, Applied biochemistry and biotechnology.

[17]  S. Shioda,et al.  Cardiovascular Malformations and Functional Changes Induced by Trichloroethylene in Developing Chick Embryos , 1993 .

[18]  N. Kashiwagi,et al.  A Method for Analyzing Data of Groundwater Contamination with Tetrachloroethylene Using Consecutive Reaction Model. , 1992 .