The application of biotechnological methods in authenticity testing.

By counterfeiting brand names in the food and drink industry as well as fraudulently labelling and selling low quality products as premium products, this sector of the industry has lost significant amounts of money and the consumer has been deceived. While it was difficult to establish certain types of fraud before the advent of modern biotechnology, DNA-based methods make an important contribution to protect high-quality brand names and protect the consumer. Several years ago, DNA technologies were considered as methods used in universities, primarily for research purpose, not so much for 'real-life' applications. However, this has changed and a number of laboratories have specialised in offering such services to the industry. This article will review DNA-based techniques commonly used for authenticity testing.

[1]  R. Veilleux,et al.  Development of microsatellite markers in potato and their use in phylogenetic and fingerprinting analyses. , 2001, Genome.

[2]  R. Veilleux,et al.  Analysis of the genetic composition of anther-derived potato by randomly amplified polymorphic DNA and simple sequence repeats. , 1995, Genome.

[3]  J. Lüthy,et al.  Polymerase chain reaction-restriction fragment length polymorphism analysis: a simple method for species identification in food. , 1995, Journal of AOAC International.

[4]  Random amplified polymorphic DNA analysis of Indian scented basmati rice (Oryza sativa L.) germplasm for identification of variability and duplicate accessions, if any , 1999, Electrophoresis.

[5]  K. Warnes,et al.  The concentration of creatine in meat, offal and commercial dog food. , 1997, Research in veterinary science.

[6]  E. K. Kemsley,et al.  Mid-infrared spectroscopy and chemometrics for the authentication of meat products. , 1999, Journal of agricultural and food chemistry.

[7]  R. Pérez-Martín,et al.  Use of restriction fragment length polymorphism to distinguish between salmon species. , 2000, Journal of agricultural and food chemistry.

[8]  M. Maher,et al.  Species-specific PCR for the identification of ovine, porcine and chicken species in meta and bone meal (MBM). , 2001, Molecular and cellular probes.

[9]  Bert Pöpping Are You Ready for [a] Roundup?—What Chemistry Has to Do with Genetic Modifications , 2001 .

[10]  E. Carrera,et al.  Identification of smoked Atlantic salmon (Salmo salar) and rainbow trout (Oncorhynchus mykiss) using PCR-restriction fragment length polymorphism of the p53 gene. , 2000, Journal of AOAC International.

[11]  S. Warwick,et al.  Evolution of SINE S1 retroposons in Cruciferae plant species. , 1997, Molecular biology and evolution.

[12]  R. Pérez-Martín,et al.  Development of a DNA-based method aimed at identifying the fish species present in food products. , 2001, Journal of agricultural and food chemistry.

[13]  R. Pérez-Martín,et al.  Comparison of different methods to produce single‐strand DNA for identification of canned tuna by single‐strand conformation polymorphism analysis , 1998, Electrophoresis.

[14]  R. Patterson,et al.  Review of current techniques for the verification of the species origin of meat. , 1990, The Analyst.

[15]  J Pauwels,et al.  IUPAC collaborative trial study of a method to detect genetically modified soy beans and maize in dried powder. , 1999, Journal of AOAC International.

[16]  G. Bryan,et al.  A PCR-based Method for the Detection of Hexaploid Bread Wheat Adulteration of Durum Wheat and Pasta , 1998 .

[17]  R. Schnabel,et al.  Validation of 15 microsatellites for parentage testing in North American bison, Bison bison and domestic cattle. , 2000, Animal genetics.

[18]  G. Remaud,et al.  Specific natural isotope profile studied by isotope ratio mass spectrometry (SNIP-IRMS): (13)C/(12)C ratios of fructose, glucose, and sucrose for improved detection of sugar addition to pineapple juices and concentrates. , 1999, Journal of agricultural and food chemistry.

[19]  R. Wood,et al.  Detection of added beet sugar in concentrated and single strength fruit juices by deuterium nuclear magnetic resonance (SNIF-NMR method): collaborative study. , 1996, Journal of AOAC International.

[20]  R. Osta,et al.  Random amplified polymorphic DNA fingerprints for identification of species in poultry pâté. , 2001, Poultry science.

[21]  C. Beattie,et al.  DEVELOPMENT OF 90 NEW BOVINE MICROSATELLITE LOCI , 2001, Animal biotechnology.