Inhibition of Trichoderma reesei cellulase by sugars and solvents

Inhibition of Trichoderma reesei cellulase by sugars (glucose, δ‐gluconolactone, and cellobiose) and solvents (ethanol, butanol, and acetone) was studied using cellulose azure. Glucose, cellobiose, ethanol, and butanol were noncompetitive inhibitors, δ‐gluconolactone was a mixed inhibitor, and acetone was a noncompetitive activator. Converting cellobiose to glucose reduces the effective inhibitor binding constant by 6 times and converting cellobiose to ethanol reduces it by 16 times.

[1]  M. Fujii,et al.  Synergism of endoenzyme and exoenzyme on hydrolysis of soluble cellulose derivatives , 1986, Biotechnology and bioengineering.

[2]  A A Huang,et al.  Kinetic studies on insoluble cellulose–cellulase system , 1975, Biotechnology and bioengineering.

[3]  G. T. Tsao,et al.  Cellobiase from Trichoderma viride: Purification, properties, kinetics, and mechanism , 1977, Biotechnology and bioengineering.

[4]  J. Quail,et al.  The reaction between 2,2-diphenyl-1-picrylhydrazyl and bromine , 1980 .

[5]  G. Caminal,et al.  Kinetic modeling of the enzymatic hydrolysis of pretreated cellulose , 1985, Biotechnology and bioengineering.

[6]  P. Beltrame,et al.  Enzymatic hydrolysis of cellulosic materials: A kinetic study , 1984, Biotechnology and bioengineering.

[7]  W. Steiner,et al.  The effect of enzyme concentration on the rate of the hydrolysis of cellulose , 1989, Biotechnology and bioengineering.

[8]  E. Ross,et al.  Mathematical model for enzymatic hydrolysis and fermentation of cellulose by Trichoderma , 1979 .

[9]  K. Das,et al.  A simplified kinetic approach to cellulose-cellulase system , 1971 .

[10]  M. Thoma,et al.  Modelling of the enzymatic hydrolysis of cellobiose and cellulose by a complex enzyme mixture of Trichoderma reesei QM 9414 , 1984, Applied Microbiology and Biotechnology.

[11]  J. Howell,et al.  Enzyme deactivation during cellulose hydrolysis , 1978 .

[12]  L. Jurasek,et al.  Hydrolysis of cellulose: mechanisms of enzymatic and acid catalysis , 1979 .

[13]  M. Holtzapple,et al.  A comparison of two empirical models for the enzymatic hydrolysis of pretreated poplar wood , 1984, Biotechnology and bioengineering.

[14]  H. Ooshima,et al.  Kinetic study on enzymatic hydrolysis of cellulose by cellulose from Trichoderma viride , 1983, Biotechnology and bioengineering.

[15]  S. R. Parr.  Some kinetic properties of the β-D-glucosidase (cellobiase) in a commercial cellulase product from Penicillium funiculosum and its relevance in the hydrolysis of cellulose , 1983 .

[16]  M Tanaka,et al.  A model of enzyme adsorption and hydrolysis of microcrystalline cellulose with slow deactivation of the adsorbed enzyme , 1988, Biotechnology and bioengineering.

[17]  M. Moo-young,et al.  Kinetics of enzymatic hydrolysis of cellulose: Analytical description of a mechanistic model , 1978, Biotechnology and bioengineering.

[18]  M. Holtzapple,et al.  The HCH‐1 model of enzymatic cellulose hydrolysis , 1984, Biotechnology and bioengineering.

[19]  A. Gusakov,et al.  A product inhibition study of cellulases from Trichoderma longibrachiatum using dyed cellulose , 1985 .

[20]  Sun Bok Lee,et al.  ENZYMATIC HYDROLYSIS OF CELLULOSE: DETERMINATION OF KINETIC PARAMETERS , 1986 .

[21]  D. B. V. Dongen,et al.  Hydrolysis of salicin by β‐glucosidase in a hollow fiber reactor , 1977 .

[22]  D. Sternberg,et al.  Immobilization of Aspergillus beta-glucosidase on chitosan , 1978, Applied and environmental microbiology.

[23]  J. Howell,et al.  Kinetics of solka floc cellulose hydrolysis by trichoderma viride cellulase , 1975 .

[24]  B. Hahn-Hägerdal,et al.  An ultrafiltration membrane reactor for obtaining experimental reaction rates at defined concentrations of inhibiting sugars during enzymatic saccharification of alkali‐pretreated sallow: Formulation of a simple empirical rate equation , 1985, Biotechnology and bioengineering.

[25]  L T Fan,et al.  Kinetic studies of enzymatic hydrolysis of insoluble cellulose: Derivation of a mechanistic kinetic model , 1983, Biotechnology and bioengineering.

[26]  M. Holtzapple,et al.  Determining the inhibition constants in the HCH‐1 model of cellulose hydrolysis , 1984, Biotechnology and bioengineering.

[27]  G. Halliwell,et al.  The nature and mode of action of the cellulolytic component C1 of Trichoderma koningii on native cellulose. , 1973, The Biochemical journal.

[28]  Stephen E. Wald,et al.  Kinetics of the enzymatic hydrolysis of cellulose , 1984, Biotechnology and bioengineering.