Molecular diagnosis of grapevine fleck virus

A digoxigenin-labelled riboprobe was developed for the detection of grapevine fleck virus (GFkV) in infected tissues of grapevine leaves, roots and canes. The probe was GFkV-specific and was successfully used for virus identification both with dot spot assays, using alkali-treated crude sap, and tissue blot assays, using cross and longitudinal sections of leaf petioles. Primers designed for the amplification by reverse transcription-polymerase chain reaction of a viral genome fragment 243 nucleotides in size, gave also positive and repeatable results. These newly developed molecular-based detection tools extend the range of available procedures for the sensitive identification of GFkV in naturally infected hosts.

[1]  G. Martelli,et al.  Identification of the agent of grapevine fleck disease , 2015 .

[2]  G. Martelli,et al.  Detection of grapevine closterovirus A in infected grapevine tissue by reverse transcription-polymerase chain reaction , 2015 .

[3]  G. Martelli,et al.  Further studies on the use of molecular probes to grapevine closterovirus A. , 2015 .

[4]  G. Martelli,et al.  Non-radioactive molecular probes for the detection of three filamentous viruses of the grapevine , 2015 .

[5]  R. Lafortezza,et al.  A non-mechanically transmissible isometric virus associated with asteroid mosaic of the grapevine , 2015 .

[6]  D. Gallitelli,et al.  Molecular identification of phytopathogenic viruses. , 1996, Methods in molecular biology.

[7]  G. Martelli,et al.  Production of monoclonal antibodies to grapevine fleck virus , 1995 .

[8]  A. Minafra,et al.  Sensitive detection of grapevine virus A, B, or leafroll-associated III from viruliferous mealybugs and infected tissue by cDNA amplification. , 1994, Journal of virological methods.

[9]  Angelantonio Minafra,et al.  Detection of grapevine leafroll‐associated closterovirus III by molecular hybridization , 1994 .

[10]  B. Walter,et al.  Elisa detection of grapevine fleck virus (GFkV) , 1993 .

[11]  T. Candresse,et al.  A highly sensitive immunocapture polymerase chain reaction method for plum pox potyvirus detection. , 1992, Journal of virological methods.

[12]  G. Martelli,et al.  Some properties of a phloem-limited non mechanically-transmissible grapevine virus. , 1990 .

[13]  John S. Hu,et al.  Characterization of closterovirus-like particles associated with grapevine leafroll disease , 1990 .

[14]  N. Navot,et al.  Rapid detection of tomato yellow leaf curl virus in squashes of plants and insect vectors. , 1989 .

[15]  G. Martelli,et al.  Ultrastructure and nature of vesiculated bodies associated with isometric virus-like particles in diseased grapevines , 1984 .

[16]  A. Maule,et al.  The application of spot hybridization to the detection of DNA and RNA viruses in plant tissues. , 1983, Journal of virological methods.

[17]  T. O. Diener,et al.  Virus degradation and nucleic acid release in single-phase phenol systems. , 1968, Archives of biochemistry and biophysics.