The bright future of single-molecule fluorescence imaging.

[1]  Th. Förster Zwischenmolekulare Energiewanderung und Fluoreszenz , 1948 .

[2]  R. Benesch,et al.  Enzymatic removal of oxygen for polarography and related methods. , 1953, Science.

[3]  D. F. Ogletree,et al.  Probing the interaction between single molecules: fluorescence resonance energy transfer between a single donor and a single acceptor , 1996, Summaries of Papers Presented at the Quantum Electronics and Laser Science Conference.

[4]  C. Seidel,et al.  Photostability of fluorescent dyes for single-molecule spectroscopy: Mechanisms and experimental methods for estimating photobleaching in aqueous solution. , 1999 .

[5]  S. Weiss Fluorescence spectroscopy of single biomolecules. , 1999, Science.

[6]  H. Peter Lu,et al.  Single-molecule Enzymology* , 1999, The Journal of Biological Chemistry.

[7]  W. E. Moerner,et al.  A Dozen Years of Single-Molecule Spectroscopy in Physics, Chemistry, and Biophysics , 2002 .

[8]  Taekjip Ha,et al.  Single-molecule three-color FRET. , 2004, Biophysical journal.

[9]  A. Ebner,et al.  Cy3B™: Improving the Performance of Cyanine Dyes , 2004, Journal of Fluorescence.

[10]  X. Zhuang Single-molecule RNA science. , 2005, Annual review of biophysics and biomolecular structure.

[11]  Jean-Pierre Clamme,et al.  Three-color single-molecule fluorescence resonance energy transfer. , 2005, Chemphyschem : a European journal of chemical physics and physical chemistry.

[12]  H. Mabuchi,et al.  Tracking-FCS: Fluorescence correlation spectroscopy of individual particles. , 2005, Optics express.

[13]  R. Tsien,et al.  The Fluorescent Toolbox for Assessing Protein Location and Function , 2006, Science.

[14]  D. Kern,et al.  Dynamic personalities of proteins , 2007, Nature.

[15]  P. Schwille,et al.  Fluorescence correlation spectroscopy: novel variations of an established technique. , 2007, Annual review of biophysics and biomolecular structure.

[16]  Nathan O'Connor,et al.  Identification of two distinct hybrid state intermediates on the ribosome. , 2007, Molecular cell.

[17]  Nam Ki Lee,et al.  Three-color alternating-laser excitation of single molecules: monitoring multiple interactions and distances. , 2007, Biophysical journal.

[18]  C. Joo,et al.  Advances in single-molecule fluorescence methods for molecular biology. , 2008, Annual review of biochemistry.

[19]  Mike Heilemann,et al.  A reducing and oxidizing system minimizes photobleaching and blinking of fluorescent dyes. , 2008, Angewandte Chemie.

[20]  Rahul Roy,et al.  A practical guide to single-molecule FRET , 2008, Nature Methods.

[21]  Colin Echeverría Aitken,et al.  An oxygen scavenging system for improvement of dye stability in single-molecule fluorescence experiments. , 2008, Biophysical journal.

[22]  James B. Munro,et al.  Mitigating unwanted photophysical processes for improved single-molecule fluorescence imaging. , 2009, Biophysical journal.

[23]  P. Tinnefeld,et al.  Correlated movement and bending of nucleic acid structures visualized by multicolor single-molecule spectroscopy. , 2009, Chemphyschem : a European journal of chemical physics and physical chemistry.

[24]  James B. Munro,et al.  Spontaneous formation of the unlocked state of the ribosome is a multistep process , 2009, Proceedings of the National Academy of Sciences.

[25]  S. Blanchard Single-molecule observations of ribosome function. , 2009, Current opinion in structural biology.

[26]  Taekjip Ha,et al.  Single-molecule four-color FRET. , 2010, Angewandte Chemie.

[27]  Michael B. Feldman,et al.  Conformational sampling of aminoacyl-tRNA during selection on the bacterial ribosome. , 2010, Journal of molecular biology.

[28]  A. Deniz,et al.  Multicolor single-molecule FRET to explore protein folding and binding. , 2010, Molecular bioSystems.

[29]  M. Levitus,et al.  Cyanine dyes in biophysical research: the photophysics of polymethine fluorescent dyes in biomolecular environments , 2010, Quarterly Reviews of Biophysics.

[30]  James B. Munro,et al.  A fast dynamic mode of the EF‐G‐bound ribosome , 2010, The EMBO journal.

[31]  Jonathan A. Javitch,et al.  Single-molecule dynamics of gating in a neurotransmitter transporter homolog , 2010, Nature.

[32]  Sanghwa Lee,et al.  Single-Molecule Three-Color FRET with Both Negligible Spectral Overlap and Long Observation Time , 2010, PloS one.

[33]  Jacob Piehler,et al.  Four-color single-molecule fluorescence with noncovalent dye labeling to monitor dynamic multimolecular complexes. , 2010, BioTechniques.

[34]  S. Blanchard,et al.  Insights into the molecular determinants of EF-G catalyzed translocation. , 2011, RNA.

[35]  Jonathan A. Javitch,et al.  Substrate-modulated gating dynamics in a Na+-coupled neurotransmitter transporter homolog , 2011, Nature.

[36]  E. Greene,et al.  Single-molecule studies of transcription: from one RNA polymerase at a time to the gene expression profile of a cell. , 2011, Journal of molecular biology.

[37]  P. Tinnefeld,et al.  Mechanisms and advancement of antifading agents for fluorescence microscopy and single-molecule spectroscopy. , 2011, Physical chemistry chemical physics : PCCP.

[38]  V. Muñoz,et al.  A photoprotection strategy for microsecond-resolution single-molecule fluorescence spectroscopy , 2011, Nature Methods.

[39]  Philip Tinnefeld,et al.  Single-molecule four-color FRET visualizes energy-transfer paths on DNA origami. , 2011, Journal of the American Chemical Society.

[40]  Mike Heilemann,et al.  Photoinduced formation of reversible dye radicals and their impact on super-resolution imaging , 2011, Photochemical & photobiological sciences : Official journal of the European Photochemistry Association and the European Society for Photobiology.

[41]  Marcel P. Bruchez,et al.  Evaluation of sCMOS cameras for detection and localization of single Cy5 molecules , 2012, Optics express.

[42]  Direct characterization of protein oligomers and their quaternary structures by single-molecule FRET. , 2012, Chemical communications.

[43]  Michael Börsch,et al.  Three-color Förster resonance energy transfer within single F₀F₁-ATP synthases: monitoring elastic deformations of the rotary double motor in real time. , 2012, Journal of biomedical optics.

[44]  Daniel S. Terry,et al.  Enhanced photostability of cyanine fluorophores across the visible spectrum , 2012, Nature Methods.

[45]  Steffen Jockusch,et al.  On the Mechanisms of Cyanine Fluorophore Photostabilization. , 2012, The journal of physical chemistry letters.

[46]  Edward A Lemke,et al.  Intramolecular three-colour single pair FRET of intrinsically disordered proteins with increased dynamic range. , 2012, Molecular bioSystems.

[47]  Jonathan A Javitch,et al.  Cyanine fluorophore derivatives with enhanced photostability , 2011, Nature Methods.

[48]  J. Puglisi,et al.  Single-molecule analysis of translational dynamics. , 2012, Cold Spring Harbor perspectives in biology.

[49]  K. Y. Han,et al.  Effect of single-base mutation on activity and folding of 10-23 deoxyribozyme studied by three-color single-molecule ALEX FRET. , 2012, The journal of physical chemistry. B.

[50]  D. Haltrich,et al.  Enzymatic Oxygen Scavenging for Photostability without pH Drop in Single-Molecule Experiments , 2012, ACS nano.

[51]  Taekjip Ha,et al.  Photophysics of fluorescent probes for single-molecule biophysics and super-resolution imaging. , 2012, Annual review of physical chemistry.

[52]  Steven F. Lee,et al.  Improved super-resolution microscopy with oxazine fluorophores in heavy water. , 2013, Angewandte Chemie.

[53]  P. Tinnefeld,et al.  Making connections — strategies for single molecule fluorescence biophysics , 2013, Current opinion in chemical biology.

[54]  Gerard Roelfes,et al.  Mechanism of intramolecular photostabilization in self-healing cyanine fluorophores. , 2013, Chemphyschem : a European journal of chemical physics and physical chemistry.

[55]  D. Toomre,et al.  Adaptive optics enables three-dimensional single particle tracking at the sub-millisecond scale , 2013 .

[56]  Michael W. Davidson,et al.  Video-rate nanoscopy enabled by sCMOS camera-specific single-molecule localization algorithms , 2013, Nature Methods.

[57]  S. Blanchard,et al.  Small-molecule photostabilizing agents are modifiers of lipid bilayer properties. , 2013, Biophysical journal.

[58]  Sarah Aufmkolk,et al.  Investigating cellular structures at the nanoscale with organic fluorophores. , 2013, Chemistry & biology.

[59]  Benjamin Schuler,et al.  Microfluidic mixer designed for performing single-molecule kinetics with confocal detection on timescales from milliseconds to minutes , 2013, Nature Protocols.

[60]  S. van de Linde,et al.  Methylene blue- and thiol-based oxygen depletion for super-resolution imaging. , 2013, Analytical chemistry.

[61]  B. Schuler,et al.  Single-molecule spectroscopy of protein folding dynamics--expanding scope and timescales. , 2013, Current opinion in structural biology.

[62]  Hak-Sung Kim,et al.  A single-molecule dissection of ligand binding to a protein with intrinsic dynamics. , 2013, Nature chemical biology.

[63]  T. Nagai,et al.  Extensive use of FRET in biological imaging. , 2013, Microscopy.

[64]  J. Elf,et al.  Single molecule methods with applications in living cells. , 2013, Current opinion in biotechnology.

[65]  Yu Lin,et al.  Single-Molecule Super-Resolution Microscopy at Video-Rate using Novel SCMOS-Specific Localization Algorithms , 2013 .

[66]  Benjamin Schuler,et al.  Single-molecule FRET of protein structure and dynamics - a primer , 2013, Journal of Nanobiotechnology.

[67]  Taekjip Ha,et al.  Single-molecule nanometry for biological physics , 2013, Reports on progress in physics. Physical Society.

[68]  Jin Chen,et al.  High-throughput platform for real-time monitoring of biological processes by multicolor single-molecule fluorescence , 2013, Proceedings of the National Academy of Sciences.

[69]  Scott C. Blanchard,et al.  Transport dynamics in a glutamate transporter homologue , 2013, Nature.

[70]  S. Jockusch,et al.  The Contribution of Reactive Oxygen Species to the Photobleaching of Organic Fluorophores , 2014, Photochemistry and photobiology.

[71]  P. Tinnefeld,et al.  Breaking the concentration limit of optical single-molecule detection. , 2014, Chemical Society reviews.

[72]  Z. Luthey-Schulten,et al.  Protein-guided RNA dynamics during early ribosome assembly , 2014, Nature.

[73]  Sanghwa Lee,et al.  Maximizing information content of single-molecule FRET experiments: multi-color FRET and FRET combined with force or torque. , 2014, Chemical Society reviews.

[74]  Mike Heilemann,et al.  Increasing the brightness of cyanine fluorophores for single-molecule and superresolution imaging. , 2014, Chemphyschem : a European journal of chemical physics and physical chemistry.

[75]  Monika A. Ciuba,et al.  Photophysical processes in single molecule organic fluorescent probes. , 2014, Chemical Society reviews.

[76]  S. Jockusch,et al.  Ultra-stable organic fluorophores for single-molecule research. , 2014, Chemical Society reviews.