Nanotechnology for the Detection and Diagnosis of Plant Pathogens

Rapid detection technologies with high sensitivity and selectivity for plant pathogens are essential to prevent disease spread with minimal loss to crop production and food quality assurance. Traditional laboratory techniques such as microscopic and cultural techniques are time-consuming and require complex sample handling. Immunological and molecular techniques are advanced but have some issues related to rapidity and signal strength. In this context, integration of immunological and molecular diagnostics with nanotechnology systems offers an alternative where all detection steps are done by a portable miniaturized device for rapid and accurate identification of plant pathogens. Further, nanomaterial synthesis by utilizing functionalized metal nanoparticles as a sensing component offer several desirable features required for pathogen detection. The sensitive nature of functionalized nanoparticles can be utilized to design phytopathogen detection devices with smart sensing capabilities for field use. This chapter provides an overview of the application of nanotechnology in the field of microbial diagnostics with special focus on plant pathogens.

[1]  Jun-yan Huang,et al.  Electrocatalytic oxidation of phytohormone salicylic acid at copper nanoparticles-modified gold electrode and its detection in oilseed rape infected with fungal pathogen Sclerotinia sclerotiorum. , 2010, Talanta.

[2]  M. Khan,et al.  Nanotechnology: Scope and Application in Plant Disease Management , 2014 .

[3]  Pedro V. Baptista,et al.  Revisiting 30 years of biofunctionalization and surface chemistry of inorganic nanoparticles for nanomedicine , 2014, Front. Chem..

[4]  W. Hudnall,et al.  Aflatoxin B1 adsorption by clays from water and corn meal , 2007 .

[5]  Alok Kumar Srivastava,et al.  Identification and Characterization of Microsatellite from Alternaria brassicicola to Assess Cross-Species Transferability and Utility as a Diagnostic Marker , 2014, Molecular Biotechnology.

[6]  N. Pourmand,et al.  Ultrasensitive mycotoxin detection by STING sensors. , 2010, Biosensors & bioelectronics.

[7]  Ashutosh Kumar,et al.  Nanotechnology in Disease Diagnostic Techniques. , 2015, Current drug metabolism.

[8]  A. Ingle,et al.  Role of nanotechnology in agriculture with special reference to management of insect pests , 2012, Applied Microbiology and Biotechnology.

[9]  Mei-xia Zhao,et al.  Application of functional quantum dot nanoparticles as fluorescence probes in cell labeling and tumor diagnostic imaging , 2015, Nanoscale Research Letters.

[10]  Evangelyn C. Alocilja,et al.  Sensitivity and specificity performance of a direct-charge transfer biosensor for detecting Bacillus cereus in selected food matrices , 2008 .

[11]  Yogeswaran Umasankar,et al.  Electrochemical detection of p-ethylguaiacol, a fungi infected fruit volatile using metal oxide nanoparticles. , 2014, The Analyst.

[12]  J. Raba,et al.  Zearalenone determination in corn silage samples using an immunosensor in a continuous-flow/stopped-flow systems. , 2010 .

[13]  S. Bhansali,et al.  Recent Advances in Detection of Ochratoxin-A , 2013 .

[14]  Alejandro Pérez-de-Luque,et al.  Nanoparticles as smart treatment-delivery systems in plants: assessment of different techniques of microscopy for their visualization in plant tissues. , 2008, Annals of botany.

[15]  Jean-Louis Marty,et al.  Development of an Electrochemical Biosensor for the Detection of Aflatoxin M1 in Milk , 2010, Sensors.

[16]  D. Spooner,et al.  All biological disciplines that depend on DNA sequence data have been fundamentally changed in the last few years, driven by the development and emergence of next-generation sequenc- , 2012 .

[17]  U. Hashim,et al.  Plant Diseases Detection Using Nanowire as Biosensor Transducer , 2013 .

[18]  Saurabh Srivastava,et al.  Nanomaterial-Based Biosensors for Food Toxin Detection , 2014, Applied Biochemistry and Biotechnology.

[19]  Beatriz Prieto-Simón,et al.  Emerging biotools for assessment of mycotoxins in the past decade , 2007 .

[20]  Jie Hu,et al.  Oligonucleotide-linked gold nanoparticle aggregates for enhanced sensitivity in lateral flow assays. , 2013, Lab on a chip.

[21]  E. Gobbi,et al.  NANOBIOTRANSDUCER FOR DETECTING FLAVESCENCE DORÉE PHYTOPLASMA , 2005 .

[22]  Chad A Mirkin,et al.  Nanostructures in biodiagnostics. , 2005, Chemical reviews.

[23]  S. Arabia,et al.  ARTICLE; AGRICULTURE AND ENVIRONMENTAL BIOTECHNOLOGY Myconanoparticles: synthesis and their role in phytopathogens management , 2015 .

[24]  Ronald W Davis,et al.  Sensitive giant magnetoresistive-based immunoassay for multiplex mycotoxin detection. , 2010, Biosensors & bioelectronics.

[25]  Ajeet Kaushik,et al.  A nanostructured cerium oxide film-based immunosensor for mycotoxin detection , 2009, Nanotechnology.

[26]  Ibtisam E. Tothill,et al.  Biosensors and nanomaterials and their application for mycotoxin determination , 2011 .

[27]  C. Chang,et al.  Fluorescence Silica Nanoprobe as a Biomarker for Rapid Detection of Plant Pathogens , 2009 .

[28]  Alberto Escarpa,et al.  Integrated electrokinetic magnetic bead-based electrochemical immunoassay on microfluidic chips for reliable control of permitted levels of zearalenone in infant foods. , 2011, The Analyst.

[29]  A. Sharma,et al.  Myconanotechnology in agriculture: a perspective , 2013, World journal of microbiology & biotechnology.

[30]  J. Marty,et al.  Enzyme-linked immunosorbent assay (ELISA) based on superparamagnetic nanoparticles for aflatoxin M1 detection. , 2008, Talanta.

[31]  T. S. Thind.  PRESIDENTIAL ADDRESS - Fungicides in crop health security–the road ahead , 2012 .

[32]  A. Ingle,et al.  Fungi as an efficient mycosystem for the synthesis of metal nanoparticles: progress and key aspects of research , 2015, Biotechnology Letters.

[33]  A. Puzyr,et al.  Neutralization of aflatoxin B1 by ozone treatment and adsorption by nanodiamonds , 2010 .

[34]  Y. Ho,et al.  Quantum dot-based nanosensors for diagnosis via enzyme activity measurement , 2013, Expert review of molecular diagnostics.

[35]  D. Collinge,et al.  A cultivation independent, PCR-based protocol for the direct identification of plant pathogens in infected plant material , 2009, European Journal of Plant Pathology.

[36]  R. Chiodini,et al.  The impact of next-generation sequencing on genomics. , 2011, Journal of genetics and genomics = Yi chuan xue bao.

[37]  V. Lattanzio,et al.  Multiplex dipstick immunoassay for semi-quantitative determination of Fusarium mycotoxins in cereals. , 2012, Analytica chimica acta.

[38]  Venkata K K Upadhyayula,et al.  Functionalized gold nanoparticle supported sensory mechanisms applied in detection of chemical and biological threat agents: a review. , 2012, Analytica chimica acta.

[39]  Wilfred Chen,et al.  Polypyrrole nanoribbon based chemiresistive immunosensors for viral plant pathogen detection , 2013 .

[40]  J. Popp,et al.  Time fluctuations and imaging in the SERS spectra of fungal hypha grown on nanostructured substrates. , 2007, The journal of physical chemistry. B.

[41]  Isao Karube,et al.  A new diagnostic method for soil-borne disease using a microbial biosensor. , 2008, Microbes and environments.

[42]  M. Steigerwald,et al.  Biosynthesis of cadmium sulphide quantum semiconductor crystallites , 1989, Nature.

[43]  Prem Lal Kashyap,et al.  Chitosan nanoparticle based delivery systems for sustainable agriculture. , 2015, International journal of biological macromolecules.

[44]  D. Branton,et al.  The potential and challenges of nanopore sequencing , 2008, Nature Biotechnology.

[45]  Steve Tung,et al.  Development and Applications of Portable Biosensors , 2015, Journal of laboratory automation.

[46]  A. M. Alvarez.  Integrated approaches for detection of plant pathogenic bacteria and diagnosis of bacterial diseases. , 2003, Annual review of phytopathology.

[47]  Jain Kk,et al.  Nanodiagnostics: application of nanotechnology in molecular diagnostics , 2003, Expert review of molecular diagnostics.

[48]  H. Bayley,et al.  Continuous base identification for single-molecule nanopore DNA sequencing. , 2009, Nature nanotechnology.

[49]  R. Etefagh,et al.  Synthesis of CuO nanoparticles and fabrication of nanostructural layer biosensors for detecting Aspergillus niger fungi , 2013 .

[50]  N. Yusof,et al.  Development of a Fluorescence Resonance Energy Transfer (FRET)-Based DNA Biosensor for Detection of Synthetic Oligonucleotide of Ganoderma boninense , 2013, Biosensors.

[51]  Chen-Han Huang,et al.  Direct detection of orchid viruses using nanorod-based fiber optic particle plasmon resonance immunosensor. , 2014, Biosensors & bioelectronics.

[52]  R. Belkhou,et al.  Tailoring magnetic vortices in nanostructures , 2010 .

[53]  A. Mukherjee,et al.  A review of the use of engineered nanomaterials to suppress plant disease and enhance crop yield , 2015, Journal of Nanoparticle Research.

[54]  Chad A Mirkin,et al.  Gold nanoparticle probes for the detection of nucleic acid targets. , 2006, Clinica chimica acta; international journal of clinical chemistry.

[55]  R. Ramasamy,et al.  Current and Prospective Methods for Plant Disease Detection , 2015, Biosensors.

[56]  C. Maycock,et al.  Quantum dot and superparamagnetic nanoparticle interaction with pathogenic fungi: internalization and toxicity profile. , 2014, ACS applied materials & interfaces.

[57]  Current correlation functions for chemical sensors based on DNA decorated carbon nanotube , 2007, cond-mat/0703245.

[58]  H. Ju,et al.  A Review of Detection Methods for the Plant Viruses , 2014 .

[59]  Ashok Kumar,et al.  An attempt to develop surface plasmon resonance based immunosensor for Karnal bunt (Tilletia indica) diagnosis based on the experience of nano-gold based lateral flow immuno-dipstick test , 2010 .

[60]  Nadejda Milanova Sertova,et al.  Application of nanotechnology in detection of mycotoxins and in agricultural sector , 2015 .

[61]  V. Adam,et al.  Quantum dots-fluorescence resonance energy transfer-based nanosensors and their application. , 2015, Biosensors & bioelectronics.

[62]  Antje J. Baeumner.  Nanosensors Identify Pathogens in Food , 2004 .

[63]  P. Solanki,et al.  Nanostructured zinc oxide platform for mycotoxin detection. , 2010, Bioelectrochemistry.

[64]  Ameeya Kumar Nayak,et al.  APPLICATIONS OF NANOTECHNOLOGY IN AGRICULTURE AND FOOD SCIENCES , 2012 .

[65]  M. Tabatabaei,et al.  Development of a quantum dots FRET-based biosensor for efficient detection of Polymyxa betae , 2012 .

[66]  P. Solanki,et al.  Antibody immobilized cysteamine functionalized-gold nanoparticles for aflatoxin detection , 2010 .

[67]  M. López,et al.  Innovative tools for detection of plant pathogenic viruses and bacteria , 2003, International microbiology : the official journal of the Spanish Society for Microbiology.

[68]  M. López,et al.  New grower-friendly methods for plant pathogen monitoring. , 2012, Annual review of phytopathology.

[69]  A. Libchaber,et al.  Single-mismatch detection using gold-quenched fluorescent oligonucleotides , 2001, Nature Biotechnology.

[70]  Minchen Chien,et al.  PEG-Labeled Nucleotides and Nanopore Detection for Single Molecule DNA Sequencing by Synthesis , 2012, Scientific Reports.

[71]  Chad A Mirkin,et al.  Bio-bar-code-based DNA detection with PCR-like sensitivity. , 2004, Journal of the American Chemical Society.

[72]  Cristina E. Davis,et al.  Advanced methods of plant disease detection. A review , 2014, Agronomy for Sustainable Development.

[73]  Rashid Bashir,et al.  BIOMEMS AND NANOTECHNOLOGY-BASED APPROACHES FOR RAPID DETECTION OF BIOLOGICAL ENTITIES , 2007 .

[74]  A. S. Nezhad.  Future of portable devices for plant pathogen diagnosis. , 2014, Lab on a chip.

[75]  James N. Turner,et al.  Upstream Migration of Xylella fastidiosa via Pilus-Driven Twitching Motility , 2005, Journal of bacteriology.

[76]  Hua Yang,et al.  Detection of foodborne pathogens using bioconjugated nanomaterials , 2008 .

[77]  Erika Check Hayden,et al.  Pint-sized DNA sequencer impresses first users , 2015, Nature.

[78]  Fengling Song,et al.  Fluorescent Nanosensors Based on Fluorescence Resonance Energy Transfer (FRET) , 2013 .

[79]  Chad A Mirkin,et al.  A bio-barcode assay for on-chip attomolar-sensitivity protein detection. , 2006, Lab on a chip.

[80]  J. Popp,et al.  Towards on-site testing of Phytophthora species , 2015 .

[81]  S. Dubas,et al.  Green synthesis of silver nanoparticles for ammonia sensing. , 2008, Talanta.

[82]  M. Alghuthaymi,et al.  Plant pathogen nanodiagnostic techniques: forthcoming changes? , 2014, Biotechnology, biotechnological equipment.

[83]  M. Tabatabaei,et al.  Detection of Candidatus Phytoplasma aurantifolia with a quantum dots fret-based biosensor. , 2012 .

[84]  P. L. Kashyap,et al.  Rapid detection and quantification of Alternaria solani in tomato , 2013 .

[85]  M. A. Alonso-Lomillo,et al.  Sensitive enzyme-biosensor based on screen-printed electrodes for Ochratoxin A. , 2010, Biosensors & bioelectronics.

[86]  Reza Ehsani,et al.  Review: A review of advanced techniques for detecting plant diseases , 2010 .

[87]  A. Heeger,et al.  Beyond superquenching: Hyper-efficient energy transfer from conjugated polymers to gold nanoparticles , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[88]  Sondeep Singh,et al.  RNA Interference: An Eco-Friendly Tool for Plant Disease Management , 2008 .

[89]  L. Horsfall,et al.  Exploring the potential of metallic nanoparticles within synthetic biology. , 2014, New biotechnology.

[90]  Elaine Ward,et al.  Molecular diagnostics for fungal plant pathogens. , 2003, Pest management science.