ANTHOCYANINS FROM BERRIES FRUITS AND THEIR PROTECTIVE EFFECT IN THE PLASMID DNA DAMAGE

Anthocyanins from berries fruits and their protective effect in the plasmid DNA damage. – Sedlák V., Konečná M., Tkáčiková Ľ., Kšonžeková P., Mydlárová-Blaščáková M., Gruľová D.,Gaľová J., Babejová A., Gogaľová Z., Avuková A., Vašková H., MirutenkoV.V., Mirutenko V.S., Kimáková T., Poráčová J. – Anthocyanins as secondary metabolites of berry fruit represent a large number of positive health effects. The physiological effect of polyphenols is a function of their chemical nature and bioavailability. Protective action is linked to their antioxidant, antimutagenic and anticancer activity. The aim of the studies was to determine the effect of ethanol and acetone extracts from berries of the fruits of Vaccinium myrtilus L., Sambucus nigra L., and Aronia melanocarpa L. on mitomycin C-induced plasmid DNA damage. We evaluated the protective effect of ethanol and acetone extracts of Vaccinium myrtilus, Sambucus nigra, and Aronia melanocarpa berry fruits in concentrations of 50, 25 and 10 μg.×ml to plasmid DNA damage induced by mitomycin C, the protective effect of anthocyanins consisted in the formation of copigmentic complex. Anthocyanins inhibited the formation and release of peroxide radicals. We found that V. myrtillus ethanol extract (50 μg/ml) showed the best protective effect on plasmid DNA. The antioxidant and antimutagenic effect of anthocyanins is based on the free radical elimination. The effect was evaluated using horizontal agarose electrophoresis.