TRACKING OF MIGRATING GLIOMA CELLS IN FEATURE SPACE

A new approach for tracking cell migration by performing tracking task in a blob feature space instead of the original image is proposed. The feature space is obtained through multi-scale blob detection, where the bright modes correspond to cells in the original image. Mode seeking is realized using a mean shift algorithm. Besides cell tracking, the method considers cell disappearing, appearing from the view, cell splitting and merging as well, the events that frequently happen in an image sequence. Results obtained from a real dataset indicate that the method performs well and with an acceptable speed. The method provides a useful tool for tracking cell migration automatically.

[1]  N. Otsu.  A threshold selection method from gray level histograms , 1979 .

[2]  Vannary Meas-Yedid,et al.  Segmentation and tracking of migrating cells in videomicroscopy with parametric active contours: a tool for cell-based drug testing , 2002, IEEE Transactions on Medical Imaging.

[3]  Dorin Comaniciu,et al.  Mean Shift: A Robust Approach Toward Feature Space Analysis , 2002, IEEE Trans. Pattern Anal. Mach. Intell..

[4]  Tony Lindeberg,et al.  Feature Detection with Automatic Scale Selection , 1998, International Journal of Computer Vision.

[5]  C Decaestecker,et al.  Dynamic characterization of glioblastoma cell motility. , 1997, Biochemical and biophysical research communications.

[6]  Douglas B. Kell,et al.  Automatic tracking of biological cells and compartments using particle filters and active contours , 2006 .

[7]  S J Lockett,et al.  Automatic detection of clustered, fluorescent-stained nuclei by digital image-based cytometry. , 1994, Cytometry.

[8]  Badrinath Roysam,et al.  A hybrid 3D watershed algorithm incorporating gradient cues and object models for automatic segmentation of nuclei in confocal image stacks , 2003, Cytometry. Part A : the journal of the International Society for Analytical Cytology.

[9]  Dorin Comaniciu,et al.  Kernel-Based Object Tracking , 2003, IEEE Trans. Pattern Anal. Mach. Intell..

[10]  Philippe Van Ham,et al.  Tracking of migrating cells under phase-contrast video microscopy with combined mean-shift processes , 2005, IEEE Transactions on Medical Imaging.