Optical coherence tomography (OCT) is a new noninvasive tomographic imaging techniques with resolution of micron scale. It has high potential for the application in many fields such as medicine, biology, material and so no. In this paper, our OCT system is shown to be successful in the study of both botanic and creatural tissue imaging. Furthermore we also use the Monte-Carlo simulation method to analyze 2D OCT image. The differences between single scattered light and multiply scattered light and also its influence on the quality of OCT image are discussed. To show an example, a conduit immersing in a high scattering medium as the model of blood vessel is simulated and the theoretical result is directly shown in the form of image. The conduit immersing in the high scattering medium can be clearly seen in the imags. Comparing with the experimental image they are basically accordant. Some results helpful for experimental research area draw through the simulation. To further enhance the resolution of OCT imaging, computer processing and image deconvolution are introduced. The depth resolution is improved by more than one order for the sample image and thus enables intracellular imaging.