A portable sensor system for bacterial concentration monitoring in metalworking fluids

Abstract. The detection of bacterial concentrations in metalworking fluids (MWFs), oil-in-water emulsions used in the cutting industries for cooling and lubrication, is important in order to extend the product life-cycle and plan its disposal according to regulations and legislations. The standard method of measuring culturable bacterial concentration is the plate count technique (PCT) that, however, has long response times and is not suitable for automatic implementation outside a laboratory. In this paper a portable sensor system that measures the bacterial concentration in liquid and semi-liquid media exploiting impedance microbiology is presented and tested for the application of MWF microbial monitoring. A set of MWF samples, taken from metalworking plants, have been tested and good agreement has been found between the system response and that of the PCT. The proposed system allows automated bacterial concentration measurements with shorter response times than the PCT (4 to 24 h vs. 24 to 72 h) and is suitable for in-the-field MWF monitoring.

[1]  K. S. B. Mannan,et al.  Viable but Nonculturable Bacteria: Food Safety and Public Health Perspective , 2013, ISRN microbiology.

[2]  B. Riccò,et al.  An automatic titration system for oil concentration measurement in metalworking fluids , 2017 .

[3]  Bansi D. Malhotra,et al.  Biosensors for pathogen detection: A smart approach towards clinical diagnosis , 2014 .

[4]  Bruno Ricco,et al.  A novel technique to control ice cream freezing by electrical characteristics analysis , 2011 .

[5]  Neil Canter New test method for detecting microbes in MWFs , 2009 .

[6]  V. Groudeva,et al.  Bacterial Microflora of Contaminated Metalworking Fluids , 2007 .

[7]  E. Bahadır,et al.  Applications of commercial biosensors in clinical, food, environmental, and biothreat/biowarfare analyses. , 2015, Analytical biochemistry.

[8]  Nico Boon,et al.  Routine bacterial analysis with automated flow cytometry. , 2013, Journal of microbiological methods.

[9]  J. Tsai,et al.  Impedimetric method for measuring ultra-low E. coli concentrations in human urine. , 2015, Biosensors & bioelectronics.

[10]  A. Rabenstein,et al.  Comparative study of microbiological monitoring of water-miscible metalworking fluids , 2015 .

[11]  X Zhou,et al.  An impedimetric method for rapid screening of cosmetic preservatives , 1995, Journal of Industrial Microbiology.

[12]  D Matteuzzi,et al.  An embedded portable biosensor system for bacterial concentration detection. , 2010, Biosensors & bioelectronics.

[13]  M. Mialich,et al.  Analysis of Body Composition: A Critical Review of the Use of Bioelectrical Impedance Analysis , 2014 .

[14]  John S. Agapiou,et al.  Metal Cutting Theory and Practice , 1996 .

[15]  Rhonda K. Miller,et al.  Inhibition of Escherichia coli O157:H7 and Salmonella enterica on spinach and identification of antimicrobial substances produced by a commercial Lactic Acid Bacteria food safety intervention. , 2014, Food microbiology.

[16]  Bruce D. Hammock,et al.  Impedance Biosensors: Applications to Sustainability and Remaining Technical Challenges , 2014, ACS sustainable chemistry & engineering.

[17]  Marco Grossi,et al.  Fast and Accurate Determination of Olive Oil Acidity by Electrochemical Impedance Spectroscopy , 2014, IEEE Sensors Journal.

[18]  H A Anderson,et al.  The spectrum of respiratory disease associated with exposure to metal working fluids. , 1998, Journal of occupational and environmental medicine.

[19]  D. Hardy,et al.  Rapid detection of microbial contamination in frozen vegetables by automated impedance measurements , 1977, Applied and environmental microbiology.

[20]  Li Ran,et al.  Prediction of state of charge of Lithium-ion rechargeable battery with electrochemical impedance spectroscopy theory , 2010, 2010 5th IEEE Conference on Industrial Electronics and Applications.

[21]  M. Lanzoni,et al.  Total Bacterial Count in Soft-Frozen Dairy Products by Impedance Biosensor System , 2009, IEEE Sensors Journal.

[22]  B. Riccò,et al.  Linear Non Iterative Sinusoidal Fitting Algorithm for Microbial Impedance Biosensor , 2012 .

[23]  Pete Peterson,et al.  Evaluation of Organic Coatings with Electrochemical Impedance Spectroscopy , 2004 .

[24]  D. Christiani,et al.  A field investigation of the acute respiratory effects of metal working fluids. I. Effects of aerosol exposures. , 1997, American journal of industrial medicine.

[25]  C. Manaia,et al.  Urban wastewater treatment plants as hotspots for antibiotic resistant bacteria and genes spread into the environment: a review. , 2013, The Science of the total environment.

[26]  Fatimah Ibrahim,et al.  The Theory and Fundamentals of Bioimpedance Analysis in Clinical Status Monitoring and Diagnosis of Diseases , 2014, Sensors.

[27]  J. H. Maindonald,et al.  Ripening of Nectarine Fruit (Changes in the Cell Wall, Vacuole, and Membranes Detected Using Electrical Impedance Measurements) , 1994, Plant physiology.

[28]  Marco Grossi,et al.  Bacterial concentration detection using a portable embedded sensor system for environmental monitoring , 2017, 2017 7th IEEE International Workshop on Advances in Sensors and Interfaces (IWASI).

[29]  Bruno Ricco,et al.  A novel electrochemical method for olive oil acidity determination , 2013, IWASI.

[30]  Marco Grossi,et al.  A Portable Sensor With Disposable Electrodes for Water Bacterial Quality Assessment , 2013, IEEE Sensors Journal.

[31]  B. Riccò,et al.  Electrical impedance spectroscopy (EIS) for biological analysis and food characterization: a review , 2017 .

[32]  A. Whiteley,et al.  Temporal dynamics and degradation activity of an bacterial inoculum for treating waste metal-working fluid. , 2004, Environmental microbiology.

[33]  Bruno Ricco,et al.  Feasibility of Lactobacilli Concentration Detec- tion in Beer by Automated Impedance Technique , 2012 .

[34]  D Matteuzzi,et al.  Detection of microbial concentration in ice-cream using the impedance technique. , 2008, Biosensors & bioelectronics.

[35]  B. Riccò,et al.  Automatic Ice-Cream Characterization by Impedance Measurements for Optimal Machine 1 Setting 2 3 , 2016 .

[36]  M. Bayramoğlu,et al.  Study on the treatment of waste metal cutting fluids using electrocoagulation , 2008 .

[37]  Marco Grossi,et al.  A portable biosensor system for bacterial concentration measurements in cow's raw milk , 2011, 2011 4th IEEE International Workshop on Advances in Sensors and Interfaces (IWASI).

[38]  R. Saha,et al.  The microbiology of metalworking fluids , 2012, Applied Microbiology and Biotechnology.

[39]  R. A. Deininger,et al.  Rapid Assessment of Microbial Hazards in Metalworking Fluids , 2005, Journal of occupational and environmental hygiene.

[40]  A. L. Koch,et al.  Turbidity measurements of bacterial cultures in some available commercial instruments. , 1970, Analytical biochemistry.

[41]  Nandakishore Rajagopalan,et al.  Membrane processing of oily streams. Wastewater treatment and waste reduction , 1998 .

[42]  Lorenzo Fedrizzi,et al.  Electrochemical impedance spectroscopy as a tool for investigating underpaint corrosion , 1996 .

[43]  O. Kanoun,et al.  SoC Li-ion battery monitoring with impedance spectroscopy , 2009, 2009 6th International Multi-Conference on Systems, Signals and Devices.

[44]  M. Lanzoni,et al.  Automatic ice-cream characterization by impedance measurements for optimal machine setting , 2012 .