论文信息 - Fermentation of Bacillus thuringiensis for exotoxin production: Process analysis study

Fermentation of Bacillus thuringiensis for exotoxin production: Process analysis study

The exotoxin produced by certain serotypes of Bacillus thuringiensis was used as a means of microbiological control of the larval development of flies. The optimal batch cultivation conditions with respect to pH, temperature, aeration, agitation, and initial concentration of growth‐limiting substrate were determined. A dynamic model describing the process was designed and fitted to the experimental data. The application of a method for estimating exotoxin and bacterial concentrations from on‐line measurable quantities such as oxygen consumption and heat production is presented.

[1] K. Linnainmaa,et al. Mutagenicity of Bacillus thuringiensis exotoxin I. Mammalian tests. , 2009, Hereditas.

[2] M. Nihtilä,et al. Practical identifiability of growth and substrate consumption models , 1977, Biotechnology and bioengineering.

[3] A Constantinides,et al. Optimization of batch fermentation processes. I. Development of mathematical models for batch penicillin fermentations. , 1970, Biotechnology and bioengineering.

[4] Petar V. Kokotovic,et al. Optimal Control of Bacterial Growth , 1972 .

[5] S. J. French,et al. A purification and some properties of an insecticidal exotoxin from Bacillus thuringiensis Berliner. , 1969, The Biochemical journal.

[6] A. Richards,et al. The production by Bacillus thuringiensis Berliner of a heat-stable substance toxic for insects. , 1959, Canadian journal of microbiology.