Oestrogen binding function of alpha 1-fetoprotein.

Abstract The discovery of a protein with high specific affinity for oestrone and oestradiol in the sera of rat and mouse at a period of intensive cellular development and sexual maturation has directed our researches primarily towards the possible role of this protein in the functions of the oestrogen hormones. One of our approaches has been a detailed study of the hormonal milieu during the perinatal growth in the rat. We have thus been able to demonstrate, along with “true” oestrogens, the presence of alternative serum ligands for alpha 1 -fetoprotein, either reactive or unreactive towards specific antioestrogen antibodies. We shall mainly concern ourselves with the preliminary characterization of the latter class of alpha 1 -fetoprotein ligands and show their ability to compete for oestrogen binding sites on oestrophilic alpha 1 -fetoproteins. Their possible role in the mechanism of action of oestrogens will be discussed.

[1]  M. Jayle,et al.  Haute affinite du serum d'embryon de souris pour les oestrogenes , 1974 .

[2]  J. Uriel,et al.  Rat alpha-fetoprotein: isolation, characterization and estrogen-binding properties. , 1974, Biochimie.

[3]  D. Burk,et al.  The Determination of Enzyme Dissociation Constants , 1934 .

[4]  I. Cameron,et al.  Relationship between functional castration and alpha-fetoprotein produced by hepatoma-bearing female rats. , 1978, Journal of reproduction and fertility.

[5]  W. H. Pearlman,et al.  Steroid-protein interaction with particular reference to testosterone binding by human serum. , 1967, The Journal of biological chemistry.

[6]  J. Uriel,et al.  Estrogen-binding properties of rat, mouse and man fetospecific serum proteins. Demonstration by immuno-autoradiographic methods. , 1972, Biochemical and biophysical research communications.

[7]  M. Jayle,et al.  Etude des constantes de liaison entre les oestrogenes et 1'α1‐foetoproteine de rat , 1972 .

[8]  A. Kaye,et al.  Oestradiol-17 beta binding proteins in the rat uterus: changes during postnatal development. , 1974, Molecular and cellular endocrinology.

[9]  R. F. Chen,et al.  Removal of fatty acids from serum albumin by charcoal treatment. , 1967, The Journal of biological chemistry.

[10]  C G BERGSTRAND,et al.  Demonstration of a new protein fraction in serum from the human fetus. , 1956, Scandinavian journal of clinical and laboratory investigation.

[11]  J. Uriel,et al.  Alpha-fetoprotein: the major high-affinity estrogen binder in rat uterine cytosols. , 1976, Proceedings of the National Academy of Sciences of the United States of America.

[12]  M. Jayle,et al.  Comparative study on the binding of estrogens by human and rat serum proteins in development. , 1974, Biochemical and biophysical research communications.

[13]  B. McEwen,et al.  Characteristics of estradiol-binding macromolecules in fetal and adult guinea pig brain cytosols. , 1977, Biology of reproduction.

[14]  M. Jayle,et al.  Rat and human embryo and post-natal sera contain a potent endogenous competitor of estrogen-rat alpha-fetoprotein interactions , 1977, Steroids.

[15]  J. Uilenbroek,et al.  PLASMA OESTRADIOL-17β AND ITS RELATIONSHIP TO SERUM FOLLICLE-STIMULATING HORMONE IN IMMATURE FEMALE RATS , 1973 .

[16]  J. Raynaud,et al.  Influence of rat estradiol binding plasma protein (EBP) on uterotrophic activity. , 1973, Steroids.

[17]  R. Masseyeff,et al.  Alpha-foetoprotein and oestrogen metabolism. I. -Influence of alpha-foetoprotein on the metabolism of steroids by rat liver microsomes in vitro. , 1976, Biochimie.

[18]  N. Cittanova,et al.  Isolation of two forms of rat alpha-fetoprotein and comparison of their binding parameters with estradiol-17beta. , 1975, Biochimica et biophysica acta.

[19]  J. Heremans,et al.  Immunochemical quantitation of antigens by single radial immunodiffusion. , 1965, Immunochemistry.

[20]  E. Baulieu,et al.  Rat estradiol binding plasma protein (EBP). , 1971, Steroids.

[21]  R. Benno,et al.  Evidence for intracellular localization of alpha-fetoprotein in the developing rat brain , 1978, Brain Research.

[22]  D. Parmelee,et al.  The presence of fatty acids in human alpha-fetoprotein. , 1978, The Journal of biological chemistry.

[23]  U. Eppenberger,et al.  Binding of steroid hormones by the 105,00 x g supernatant fraction from homogenates of rat skin and variations during the hair cycle. , 1972, The Journal of biological chemistry.

[24]  E. Baulieu,et al.  alpha-Fetoprotein is not a component of the estradiol receptor of the rat uterus. , 1977, Proceedings of the National Academy of Sciences of the United States of America.

[25]  G. Scatchard,et al.  THE ATTRACTIONS OF PROTEINS FOR SMALL MOLECULES AND IONS , 1949 .

[26]  R. King,et al.  Steroid-cell interactions , 1974 .

[27]  M. Jayle,et al.  Rat iso-alpha1-fetoproteins purification and interaction with estradiol-17β , 1977, Steroids.

[28]  R. Masseyeff,et al.  Binding of estrogens to molecular variants of rat alpha‐fetoprotein , 1977, FEBS letters.