ELECTRON MICROSCOPY OF ULTRA-THIN SECTIONS OF BACTERIA I

In the course of cytological studies of a few selected species of bacteria by the techniques of ultra-thin sectioning and electron microscopy, some new and si8nificant information has been obtained concerning cellular division in Bacillus cereus. The cytoloqg of bacterial cellular division inYneS-a_ number of structures aiid ev-ts which are beyond the resolution limit of the light microscope. The complexity of these structures and -the psi'bl1e variatiow whfch differences in species, age of culture, and growth conditions may introduce have made it impossible to make reliable interpretations by indirect methods. This has led to considerable confuwsion as is apparent from any review of the literature. It is hoped that some of the observations made in the present investigation will remove a little of that confusion and point the way to much greater contributions of the method in the future.

[1]  G. Palade,et al.  The fine structure of mitochondria , 1952, The Anatomical record.

[2]  C. E. Clifton,et al.  NUCLEAR CHANGES IN LIVING CELLS OF A VARIANT OF BACILLUS ANTHRACIS , 1952, Journal of bacteriology.

[3]  E. Delamater,et al.  THE NUCLEAR CYTOLOGY OF SPORULATION IN BACILLUS MEGATERIUM , 1952, Journal of bacteriology.

[4]  L. Winterscheid,et al.  EVIDENCE SUGGESTING THAT THE GRANULES OF MYCOBACTERIA ARE MITOCHONDRIA , 1951, Journal of bacteriology.

[5]  E. Delamater,et al.  PRELIMINARY REPORT OF TRUE MITOSIS IN THE VEGETATIVE CELL OF BACILLUS MEGATHERIUM , 1951 .

[6]  W. Cassel.  A PROCEDURE FOR THE SIMULTANEOUS DEMONSTRATION OF THE CELL WALLS AND CHROMATINIC BODIES OF BACTERIA , 1951, Journal of bacteriology.

[7]  E. Delamater.  A staining and dehydrating procedure for the handling of microorganisms. , 1951, Stain technology.

[8]  L. Hewitt.  Effect of cultural conditions on bacterial cytology. , 1951, Journal of general microbiology.

[9]  H. Stempen.  DEMONSTRATION OF THE CHROMATINIC BODIES OF ESCHERICHIA COLI AND PROTEUS VULGARIS WITH THE AID OF THE PHASE CONTRAST MICROSCOPE , 1950, Journal of bacteriology.

[10]  S. Mudd,et al.  ELECTRON AND LIGHT MICROSCOPIC STUDIES OF BACTERIAL NUCLEI I , 1950, Journal of bacteriology.

[11]  F. Johnson,et al.  NUCLEI AND LARGE BODIES OF LUMINOUS BACTERIA IN RELATION TO SALT CONCENTRATION, OSMOTIC PRESSURE, TEMPERATURE, AND URETHANE , 1949, Journal of bacteriology.

[12]  J. Hillier,et al.  INTERNAL STRUCTURE AND NUCLEI IN CELLS OF ESCHERICHIA COLI AS SHOWN BY IMPROVED ELECTRON MICROSCOPIC TECHNIQUES , 1949, Journal of bacteriology.

[13]  J. Broadhurst,et al.  ELEMENTS OF BACTERIAL CYTOLOGY , 1945 .

[14]  G. Knaysi.  Observations on the Cell Division of Some Yeasts and Bacteria , 1941, Journal of bacteriology.

[15]  F. S. Sjostrand.  Electron Microscopy of Mitochondria and Cytoplasmic Double Membranes: Systems of Double Membranes in the Cytoplasm of Certain Tissue Cell , 1953, Nature.

[16]  S. Mudd,et al.  The occurrence of mitosis in the vegetative phase of bacillus megatherium , 1951 .

[17]  B. Delaporte.  Observations on the cytology of bacteria. , 1950, Advances in genetics.

[18]  K. Bisset.  The Cytology of Smooth and Rough Variation in Bacteria. , 1948 .