B-Cell ELISpot Assay to Quantify Antigen-Specific Antibody-Secreting Cells in Human Peripheral Blood Mononuclear Cells.
暂无分享,去创建一个
[1] Shiang-Jong Tzeng,et al. The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity. , 2016, Journal of visualized experiments : JoVE.
[2] Greg Finak,et al. Pooled-Peptide Epitope Mapping Strategies Are Efficient and Highly Sensitive: An Evaluation of Methods for Identifying Human T Cell Epitope Specificities in Large-Scale HIV Vaccine Efficacy Trials , 2016, PloS one.
[3] Shiang-Jong Tzeng,et al. FcγRIIB mediates antigen-independent inhibition on human B lymphocytes through Btk and p38 MAPK , 2015, Journal of Biomedical Science.
[4] Christine A. Goetz,et al. Comparative Multi-Donor Study of IFNγ Secretion and Expression by Human PBMCs Using ELISPOT Side-by-Side with ELISA and Flow Cytometry Assays , 2015, Cells.
[5] C. Czerkinsky,et al. Enzyme-linked immunospot assays for direct ex vivo measurement of vaccine-induced human humoral immune responses in blood , 2013, Nature Protocols.
[6] Peter D. Crompton,et al. High efficiency human memory B cell assay and its application to studying Plasmodium falciparum-specific memory B cells in natural infections. , 2012, Journal of immunological methods.
[7] P. Lipsky,et al. Isolation of Human B Cell Populations , 2006, Current protocols in immunology.
[8] R. Ahmed,et al. Tracking human antigen-specific memory B cells: a sensitive and generalized ELISPOT system. , 2004, Journal of immunological methods.
[9] B. Chernow,et al. Use of different anticoagulants in test tubes for analysis of blood lactate concentrations: Part 2. Implications for the proper handling of blood specimens obtained from critically ill patients. , 1997, Critical care medicine.
[10] O Ouchterlony,et al. A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells. , 1983, Journal of immunological methods.
[11] L. Frelin,et al. Methods to Evaluate Novel Hepatitis C Virus Vaccines. , 2016, Methods in molecular biology.
[12] C. Krawczyk,et al. Detecting Secreted Analytes from Immune Cells: An Overview of Technologies. , 2016, Methods in molecular biology.
[13] M. Sedegah. The Ex Vivo IFN-γ Enzyme-Linked Immunospot (ELISpot) Assay. , 2015, Methods in molecular biology.
[14] Markus Wulf,et al. Identification and validation of T-cell epitopes using the IFN-gamma ELISPOT assay. , 2009, Methods in molecular biology.