B-Cell ELISpot Assay to Quantify Antigen-Specific Antibody-Secreting Cells in Human Peripheral Blood Mononuclear Cells.

[1]  Shiang-Jong Tzeng,et al.  The Isolation, Differentiation, and Quantification of Human Antibody-secreting B Cells from Blood: ELISpot as a Functional Readout of Humoral Immunity. , 2016, Journal of visualized experiments : JoVE.

[2]  Greg Finak,et al.  Pooled-Peptide Epitope Mapping Strategies Are Efficient and Highly Sensitive: An Evaluation of Methods for Identifying Human T Cell Epitope Specificities in Large-Scale HIV Vaccine Efficacy Trials , 2016, PloS one.

[3]  Shiang-Jong Tzeng,et al.  FcγRIIB mediates antigen-independent inhibition on human B lymphocytes through Btk and p38 MAPK , 2015, Journal of Biomedical Science.

[4]  Christine A. Goetz,et al.  Comparative Multi-Donor Study of IFNγ Secretion and Expression by Human PBMCs Using ELISPOT Side-by-Side with ELISA and Flow Cytometry Assays , 2015, Cells.

[5]  C. Czerkinsky,et al.  Enzyme-linked immunospot assays for direct ex vivo measurement of vaccine-induced human humoral immune responses in blood , 2013, Nature Protocols.

[6]  Peter D. Crompton,et al.  High efficiency human memory B cell assay and its application to studying Plasmodium falciparum-specific memory B cells in natural infections. , 2012, Journal of immunological methods.

[7]  P. Lipsky,et al.  Isolation of Human B Cell Populations , 2006, Current protocols in immunology.

[8]  R. Ahmed,et al.  Tracking human antigen-specific memory B cells: a sensitive and generalized ELISPOT system. , 2004, Journal of immunological methods.

[9]  B. Chernow,et al.  Use of different anticoagulants in test tubes for analysis of blood lactate concentrations: Part 2. Implications for the proper handling of blood specimens obtained from critically ill patients. , 1997, Critical care medicine.

[10]  O Ouchterlony,et al.  A solid-phase enzyme-linked immunospot (ELISPOT) assay for enumeration of specific antibody-secreting cells. , 1983, Journal of immunological methods.

[11]  L. Frelin,et al.  Methods to Evaluate Novel Hepatitis C Virus Vaccines. , 2016, Methods in molecular biology.

[12]  C. Krawczyk,et al.  Detecting Secreted Analytes from Immune Cells: An Overview of Technologies. , 2016, Methods in molecular biology.

[13]  M. Sedegah The Ex Vivo IFN-γ Enzyme-Linked Immunospot (ELISpot) Assay. , 2015, Methods in molecular biology.

[14]  Markus Wulf,et al.  Identification and validation of T-cell epitopes using the IFN-gamma ELISPOT assay. , 2009, Methods in molecular biology.