A Paper‐based Mitochondrial Electrochemical Biosensor for Pesticide Detection

In this article, we detail a paper-based three-electrode electrochemical biosensor using a mitochondria modified Toray carbon paper working electrode. Cyclic voltammetry performed on the paper-based biosensor and similar electrodes in a common laboratory setup (not in an integrated paper-based device) compare favorably. In addition, instant detection of malathion with a detection limit of 20 nM by cyclic voltammetry is demonstrated, showing the device can potentially be used as a portable platform for pesticides detection.

[1]  Megan Bang,et al.  The cultural side of science communication , 2014, Proceedings of the National Academy of Sciences.

[2]  Shelley D. Minteer,et al.  Organelle-based biofuel cells: Immobilized mitochondria on carbon paper electrodes , 2008 .

[3]  S. Hossain,et al.  β-Galactosidase-based colorimetric paper sensor for determination of heavy metals. , 2011, Analytical chemistry.

[4]  M R Guascito,et al.  Heavy metal determination by biosensors based on enzyme immobilised by electropolymerisation. , 2005, Biosensors & bioelectronics.

[5]  M. Brand Uncoupling to survive? The role of mitochondrial inefficiency in ageing , 2000, Experimental Gerontology.

[6]  J. Quevedo,et al.  Mitochondrial Respiratory Dysfunction and Oxidative Stress after Chronic Malathion Exposure , 2006, Neurochemical Research.

[7]  A. West,et al.  A dual enzyme electrochemical assay for the detection of organophosphorus compounds using organophosphorus hydrolase and horseradish peroxidase , 2011 .

[8]  E. Podestà,et al.  Alkaline phosphatase inhibition based electrochemical sensors for the detection of pesticides , 2004 .

[9]  Rolf D. Schmid,et al.  A disposable multielectrode biosensor for rapid simultaneous detection of the insecticides paraoxon and carbofuran at high resolution , 1999 .

[10]  S. Ōmura,et al.  Atpenins, potent and specific inhibitors of mitochondrial complex II (succinate-ubiquinone oxidoreductase) , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[11]  T. M. Reddy,et al.  Development of AChE biosensor for the determination of methyl parathion and monocrotophos in water and fruit samples: A cyclic voltammetric study , 2012 .

[12]  J Rishpon,et al.  Immobilized parathion hydrolase: an amperometric sensor for parathion. , 2000, Analytical chemistry.

[13]  John D Brennan,et al.  Reagentless bidirectional lateral flow bioactive paper sensors for detection of pesticides in beverage and food samples. , 2009, Analytical chemistry.

[14]  J. Mills,et al.  Analysis of pesticide residues in food using gas chromatography—tandem mass spectrometry with a benchtop ion trap mass spectrometer , 1994 .

[15]  L. M. Davies,et al.  Development of a bioactive paper sensor for detection of neurotoxins using piezoelectric inkjet printing of sol-gel-derived bioinks. , 2009, Analytical chemistry.

[16]  M. Scharf,et al.  Mitochondrial impacts of insecticidal formate esters in insecticide-resistant and insecticide-susceptible Drosophila melanogaster. , 2009, Pest management science.

[17]  Dan Du,et al.  Acetylcholinesterase biosensor design based on carbon nanotube-encapsulated polypyrrole and polyaniline copolymer for amperometric detection of organophosphates. , 2010, Biosensors & bioelectronics.

[18]  A. L. Hart,et al.  The response of screen-printed enzyme electrodes containing cholinesterases to organo-phosphates in solution and from commercial formulations , 1997 .

[19]  A Mulchandani,et al.  Biosensor for direct determination of organophosphate nerve agents using recombinant Escherichia coli with surface-expressed organophosphorus hydrolase. 2. Fiber-optic microbial biosensor. , 1998, Analytical chemistry.

[20]  Isao Karube,et al.  Microfabricated Liquid Junction Ag/AgCl Reference Electrode and Its Application to a One-Chip Potentiometric Sensor , 1999 .

[21]  M. Solioz,et al.  The pyrethroids permethrin and cyhalothrin are potent inhibitors of the mitochondrial complex I. , 1997, The Journal of pharmacology and experimental therapeutics.

[22]  Mark Ellisman,et al.  Mitochondria frozen with trehalose retain a number of biological functions and preserve outer membrane integrity , 2007, Cell Death and Differentiation.

[23]  Sandeep Kumar Jha,et al.  Optical microbial biosensor for detection of methyl parathion pesticide using Flavobacterium sp. whole cells adsorbed on glass fiber filters as disposable biocomponent. , 2006, Biosensors & bioelectronics.

[24]  Shelley D. Minteer,et al.  Self-Powered Herbicide Biosensor Utilizing Thylakoid Membranes , 2013 .

[25]  L Polese,et al.  Determination of pesticide residues in coconut water by liquid-liquid extraction and gas chromatography with electron-capture plus thermionic specific detection and solid-phase extraction and high-performance liquid chromatography with ultraviolet detection. , 2002, Journal of chromatography. A.

[26]  Yong Liu,et al.  Audio jack based miniaturized mobile phone electrochemical sensing platform , 2015 .

[27]  S. Yao,et al.  Upconversion nanoparticle-based fluorescence resonance energy transfer assay for organophosphorus pesticides. , 2015, Biosensors & bioelectronics.

[28]  Ò. Miró,et al.  Reversible inhibition of mitochondrial complex IV activity in PBMC following acute smoking , 2004, European Respiratory Journal.

[29]  J. Casida Pest toxicology: the primary mechanisms of pesticide action. , 2009, Chemical research in toxicology.

[30]  M. Brand,et al.  High Throughput Microplate Respiratory Measurements Using Minimal Quantities Of Isolated Mitochondria , 2011, PloS one.

[31]  S. Minteer,et al.  Mitochondrial-based voltammetric sensor for pesticides , 2012 .

[32]  S. Minteer,et al.  Understanding the mechanism of direct electrochemistry of mitochondria-modified electrodes from yeast, potato and bovine sources at carbon paper electrodes , 2013 .

[33]  D. Weisenburger Human health effects of agrichemical use. , 1993, Human pathology.

[34]  P. Atanassov,et al.  Standardized microbial fuel cell anodes of silica-immobilized Shewanella oneidensis. , 2010, Chemical communications.

[35]  K R Rogers,et al.  Biosensors for direct determination of organophosphate pesticides. , 2001, Biosensors & bioelectronics.

[36]  Jyh-Myng Zen,et al.  Disposable electrochemical sensor for determination of nitroaromatic compounds by a single-run approach. , 2006, Analytical chemistry.

[37]  Na Wang,et al.  An ultra-sensitive acetylcholinesterase biosensor based on reduced graphene oxide-Au nanoparticles-β-cyclodextrin/Prussian blue-chitosan nanocomposites for organophosphorus pesticides detection. , 2015, Biosensors & bioelectronics.

[38]  Wilfred Chen,et al.  Functional analysis of organophosphorus hydrolase variants with high degradation activity towards organophosphate pesticides. , 2006, Protein engineering, design & selection : PEDS.