Distribution of proenkephalin‐derived peptides in the brain of Rana esculenta

The immunocytochemical distribution of authentic proenkephalin‐containing perikarya and nerve fibers in the brain of Rana esculenta was determined with antisera directed toward different epitopes of preproenkephalin. The pattern of proenkephalinlike immunoreactivity was similar with antisera directed toward [Met5]‐enkephalin, [Met5]‐enkephalin‐Arg6, [Met5]‐enkephalin‐Arg6‐Phe7, [Leu5]‐enkephalin, and metorphamide; however, the intensity of the labelling varied depending on the target antigen.

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