Evaluation of industrial dairy waste (milk dust powder) for acetone-butanol-ethanol production by solventogenic Clostridium species

Readily available inexpensive substrate with high product yield is the key to restoring acetone-butanol-ethanol (ABE) fermentation to economic competitiveness. Lactose-replete cheese whey tends to favor the production of butanol over acetone. In the current study, we investigated the fermentability of milk dust powder with high lactose content, for ABE production by Clostridium acetobutylicum and Clostridium beijerinckii. Both microorganisms produced 7.3 and 5.8 g/L of butanol respectively, with total ABE concentrations of 10.3 and 8.2 g/L, respectively. Compared to fermentation with glucose, fermentation of milk dust powder increased butanol to acetone ratio by 16% and 36% for C. acetobutylicum and C. beijerinckii, respectively. While these results demonstrate the fermentability of milk dust powder, the physico-chemical properties of milk dust powder appeared to limit sugar utilization, growth and ABE production. Further work aimed at improving the texture of milk dust powder-based medium would likely improve lactose utilization and ABE production.

[1]  K. Prather,et al.  Engineering alternative butanol production platforms in heterologous bacteria. , 2009, Metabolic Engineering.

[2]  Christian J Sund,et al.  Transcriptional analysis of differential carbohydrate utilization by Clostridium acetobutylicum. , 2010, Microbiology.

[3]  C. E. Voget,et al.  Butanol production from apple pomace , 2005, Biotechnology Letters.

[4]  Anastassios G. Stamatis,et al.  Biotechnological Utilization with a Focus on Anaerobic Treatment of Cheese Whey: Current Status and Prospects , 2012 .

[5]  Genta Kobayashi,et al.  Production of Acetone–Butanol–Ethanol (ABE) in Direct Fermentation of Cassava by Clostridium saccharoperbutylacetonicum N1-4 , 2010, Applied biochemistry and biotechnology.

[6]  Anneli Petersson,et al.  Increased tolerance and conversion of inhibitors in lignocellulosic hydrolysates by Saccharomyces cerevisiae , 2007 .

[7]  T. Ezeji,et al.  Transcriptional analysis of Clostridium beijerinckii NCIMB 8052 to elucidate role of furfural stress during acetone butanol ethanol fermentation , 2013, Biotechnology for Biofuels.

[8]  Kees van Wingerden,et al.  Does your facility have a dust problem: Methods for evaluating dust explosion hazards , 2011 .

[9]  I. Veliky,et al.  Production of acetone - butanol from acid whey , 2004, Biotechnology Letters.

[10]  I. Maddox.  Production of n-butanol from whey filtrate using clostridium acetobutylicum N.C.I.B. 2951 , 1980, Biotechnology Letters.

[11]  N. Qureshi,et al.  Butanol production using Clostridium beijerinckii BA101 hyper-butanol producing mutant strain and recovery by pervaporation , 2000, Applied biochemistry and biotechnology.

[12]  D. Bender.  A Dictionary of Food and Nutrition , 2005 .

[13]  W. Mitchell,et al.  Analysis of the Mechanism and Regulation of Lactose Transport and Metabolism in Clostridium acetobutylicum ATCC 824 , 2007, Applied and Environmental Microbiology.

[14]  Fabio Napoli.  Development of an Integrated Bioprocess For Butanol Production , 2009 .

[15]  Mervat I. Foda,et al.  Study the Suitability of Cheese Whey for Bio-Butanol Production by Clostridia , 2010 .

[16]  E. M. Brown,et al.  Nomenclature of the proteins of cows' milk--sixth revision. , 1965, Journal of dairy science.

[17]  T. Mawhinney,et al.  Determination of thirteen common elements in food samples by inductively coupled plasma atomic emission spectrometry: comparison of five digestion methods. , 2000, Journal of AOAC International.

[18]  T. Ezeji,et al.  Biotransformation of furfural and 5-hydroxymethyl furfural (HMF) by Clostridium acetobutylicum ATCC 824 during butanol fermentation. , 2012, New biotechnology.

[19]  T. Ezeji,et al.  Use of Proteomic Analysis To Elucidate the Role of Calcium in Acetone-Butanol-Ethanol Fermentation by Clostridium beijerinckii NCIMB 8052 , 2012, Applied and Environmental Microbiology.

[20]  T. Ezeji,et al.  Impact of syringaldehyde on the growth of Clostridium beijerinckii NCIMB 8052 and butanol production , 2012, 3 Biotech.

[21]  I. Maddox,et al.  Production of solvents (ABE fermentation) from whey permeate by continuous fermentation in a membrane bioreactor , 1989 .

[22]  H. Bahl,et al.  Nutritional Factors Affecting the Ratio of Solvents Produced by Clostridium acetobutylicum , 1986, Applied and environmental microbiology.

[23]  J. Russell,et al.  The effects of fermentation acids on bacterial growth. , 1998, Advances in microbial physiology.

[24]  T. Ezeji,et al.  Acetone production in solventogenic Clostridium species: new insights from non-enzymatic decarboxylation of acetoacetate , 2011, Applied Microbiology and Biotechnology.

[25]  Nathan D. Price,et al.  Achievements and perspectives to overcome the poor solvent resistance in acetone and butanol-producing microorganisms , 2010, Applied Microbiology and Biotechnology.

[26]  Wayne H. Thompson,et al.  Test methods for the examination of composting and compost , 1998 .

[27]  Shiyuan Hu,et al.  Identification and inactivation of pleiotropic regulator CcpA to eliminate glucose repression of xylose utilization in Clostridium acetobutylicum. , 2010, Metabolic engineering.

[28]  Yixue Li,et al.  Large number of phosphotransferase genes in the Clostridium beijerinckii NCIMB 8052 genome and the study on their evolution , 2010, BMC Bioinformatics.

[29]  T. Ezeji,et al.  Fermentation of dried distillers' grains and solubles (DDGS) hydrolysates to solvents and value-added products by solventogenic clostridia. , 2008, Bioresource technology.

[30]  T. Ezeji,et al.  Production of butanol from starch-based waste packing peanuts and agricultural waste , 2002, Journal of Industrial Microbiology and Biotechnology.

[31]  R. Bajpai,et al.  Fermentation of cheese whey by a mixed culture ofClostridium beijerinckii andBacillus cereus , 1988, Journal of Industrial Microbiology.

[32]  N. Qureshi,et al.  Reduction in Butanol Inhibition by Perstraction: Utilization of Concentrated Lactose/Whey Permeate by Clostridium acetobutylicum to Enhance Butanol Fermentation Economics , 2005 .

[33]  G. Gottschalk,et al.  Physiological Events in Clostridium acetobutylicum during the Shift from Acidogenesis to Solventogenesis in Continuous Culture and Presentation of a Model for Shift Induction , 1992, Applied and environmental microbiology.

[34]  H. Blaschek,et al.  Buffering as a means for increasing growth and butanol production byClostridium acetobutylicum , 1988, Journal of Industrial Microbiology.