Molecular Markers for Self- compatibility in Japanese Apricot (Prunus mume)

Self-compatible cultivars of Japanese apricot ( Prunus mume Sieb. et Zucc.) have a horticultural advantage over self-incompatible ones because no pollinizer is required. Self-incompatibility is gametophytic, as in other Prunus species. We searched for molecular markers to identify self-compatible cultivars based on the information about S-ribonucleases (S-RNases) of other Prunus species. Total DNA isolated from five self-incompatible and six self-compatible cultivars were PCR-amplified by oligonucle- otide primers designed from conserved regions of Prunus S-RNases. Self-compatible cultivars exhibited a common band of ≈1.5 kbp. Self-compatible cultivars also showed a common band of ≈12.1 kbp when genomic DNA digested with HindIII was probed with the cDNA encoding S 2 -RNase of sweet cherry (Prunus avium L.). These results suggest that self-compatible cultivars of Japanese apricot have a common S-RNase allele that can be used as a molecular marker for self-compatibility.

[1]  H. Hirano,et al.  Identification of self-incompatibility genotypes of almond by allele-specific PCR analysis , 2000, Theoretical and Applied Genetics.

[2]  R. Tao,et al.  Identification and cDNA Cloning for S-RNases in Self-incompatible Japanese Plum (Prunus salicina Lindl. cv. Sordum) , 1999 .

[3]  F. Dicenta,et al.  A stylar ribonuclease assay to detect self-compatible seedlings in almond progenies , 1999, Theoretical and Applied Genetics.

[4]  J. Golz,et al.  A molecular description of mutations affecting the pollen component of the Nicotiana alata S locus. , 1999, Genetics.

[5]  K. Inoue,et al.  PCR-based method for identifying the S-genotypes of Japanese pear cultivars , 1999, Theoretical and Applied Genetics.

[6]  R. Tao,et al.  Molecular Typing of S-alleles through Identification, Characterization and cDNA Cloning for S-RNases in Sweet Cherry , 1999 .

[7]  H. Hirano,et al.  Cloning and characterization of cDNAs encoding S-RNases from almond (Prunus dulcis): primary structural features and sequence diversity of the S-RNases in Rosaceae , 1998, Molecular and General Genetics MGG.

[8]  T. Nishio,et al.  Style‐specific self‐compatible mutation caused by deletion of the S‐RNase gene in Japanese pear (Pyrus serotina) , 1997 .

[9]  T. Gradziel,et al.  Identification of stylar RNases associated with gametophytic self-incompatibility in almond (Prunus dulcis). , 1997, Plant & cell physiology.

[10]  S. Y. Lee,et al.  The 5' flanking sequences of two S alleles in Lycopersicon peruvianum are highly heterologous but contain short blocks of homologous sequences. , 1995, Plant & cell physiology.

[11]  L. Rowland,et al.  Use of polyethylene glycol for purification of DNA from leaf tissue of woody plants. , 1993, BioTechniques.

[12]  H. Hirano,et al.  Self-Incompatibility-Related RNases in Styles of Japanese Pear (Pyrus serotina Rehd.) , 1992 .

[13]  E. Pahlich,et al.  A rapid DNA isolation procedure for small quantities of fresh leaf tissue , 1980 .

[14]  J. Janick,et al.  Advances in fruit breeding. , 1975 .