Epstein-Barr viral DNA in tissues of Hodgkin's disease.

Tissue specimens from 21 cases of Hodgkin's disease were examined for the presence of Epstein-Barr virus (EBV) and cytomegalovirus DNA by molecular hybridization techniques. EBV DNA was detected in 4 cases, including 2 of 8 cases which had been previously shown to contain clonal immunoglobulin gene rearrangements. Two of the cases containing EBV DNA were of the nodular sclerosing type and had received prior therapy; the other 2 were classified as mixed cellularity Hodgkin's disease and had not received therapy before the biopsy tissue was obtained. Analyses of the terminal portions of EBV genomes indicated a monoclonal or oligoclonal proliferation of EBV-infected cells in the tissues studied. In contrast, none of the 21 cases had detectable cytomegalovirus DNA sequences. The identification of EBV DNA may reflect the proliferation of lymphoblastoid cells due to the reduced immune competence frequently noted in Hodgkin's disease or may indicate the presence of EBV genomes within Reed-Sternberg cells.

[1]  L. Young,et al.  Human epithelial cell expression of an Epstein-Barr virus receptor. , 1987, The Journal of general virology.

[2]  Hiroshi Sato,et al.  Biological Properties of Epstein-Barr Virus Recovered from Epithelial Cells Transfected with DNA Prepared from a Nasopharyngeal Carcinoma-Derived EBV , 1987 .

[3]  N. Raab-Traub,et al.  The structure of the termini of the Epstein-Barr virus as a marker of clonal cellular proliferation , 1986, Cell.

[4]  R. Warnke,et al.  Immunoglobulin gene rearrangements in Hodgkin's disease. , 1986, Human pathology.

[5]  D. Fearon,et al.  The C3d Receptor Identified by the HB-5 Monoclonal Antibody: Expression and Role as a Receptor for Epstein-Barr Virus , 1986 .

[6]  M. Epstein,et al.  The Epstein-Barr virus : recent advances , 1986 .

[7]  S. Poppema,et al.  Lymphadenopathy morphologically consistent with Hodgkin's disease associated with Epstein-Barr virus infection. , 1985, American journal of clinical pathology.

[8]  Robert Walgate,et al.  Proliferation , 1985, Nature.

[9]  R. Warnke,et al.  Epstein-Barr virus-associated B-cell proliferations of diverse clonal origins after bone marrow transplantation in a 12-year-old patient with severe combined immunodeficiency. , 1985, The New England journal of medicine.

[10]  E. Jaffe,et al.  Phenotypic expression of Hodgkin's and Reed-Sternberg cells in Hodgkin's disease. , 1985, The American journal of pathology.

[11]  J. Sklar,et al.  LYMPHOPROLIFERATIVE DISORDERS IN CARDIAC TRANSPLANT RECIPIENTS ARE MULTICLONAL LYMPHOMAS , 1984, The Lancet.

[12]  H. Wolf,et al.  Persistence of Epstein-Barr virus in the parotid gland , 1984, Journal of virology.

[13]  N. Gutensohn,et al.  A population‐based case‐control study of EBV and other viral antibodies among persons with Hodgkin's disease and their siblings , 1984, International journal of cancer.

[14]  R. Warnke,et al.  Monoclonality of lymphoproliferative lesions in cardiac-transplant recipients. Clonal analysis based on immunoglobulin-gene rearrangements. , 1984, The New England journal of medicine.

[15]  E. Kieff,et al.  Long internal direct repeat in Epstein-Barr virus DNA , 1982, Journal of virology.

[16]  B. Fleckenstein,et al.  Cloning of the complete human cytomegalovirus genome in cosmids. , 1982, Gene.

[17]  W. Henle,et al.  Expression of Epstein-Barr viral early antigen in monolayer tissue cultures after transfection with viral DNA and DNA fragments , 1981, Journal of virology.

[18]  J. Najarian,et al.  The Epstein-Barr virus in the pathogenesis of posttransplant lymphoproliferative disorders. Clinical, pathologic, and virologic correlation. , 1981, Surgery.

[19]  N. Gutensohn,et al.  Childhood social environment and Hodgkin's disease. , 1981, The New England journal of medicine.

[20]  H. Wolf,et al.  Epstein–Barr virus-induced cell fusion , 1980, Nature.

[21]  G. Hayward,et al.  Organization of repeated regions within the Epstein-Barr virus DNA molecule , 1980, Journal of virology.

[22]  F C Kafatos,et al.  Determination of nucleic acid sequence homologies and relative concentrations by a dot hybridization procedure. , 1979, Nucleic acids research.

[23]  G. de‐Thé,et al.  Infectious mononucleosis and Hodgkin's disease , 1978, International journal of cancer.

[24]  J. Holý,et al.  [Epidemiology of Hodgkin's disease]. , 1977, Ceskoslovenska pediatrie.

[25]  N. Gutensohn,et al.  Epidemiology of Hodgkin's disease in the young , 1977, International journal of cancer.

[26]  T. Lindahl,et al.  Epstein-Barr virus DNA in human lymphoid cell lines: in vitro conversion. , 1976, Virology.

[27]  E. Southern Detection of specific sequences among DNA fragments separated by gel electrophoresis. , 1975, Journal of molecular biology.

[28]  G. Klein,et al.  Relationship between Epstein‐Barr virus (EBV) DNA and the EBV‐determined nuclear antigen (EBNA) in Burkitt lymphoma biopsies and other lymphoproliferative malignancies , 1974, International journal of cancer.

[29]  H. Hausen,et al.  Attempts to detect virus‐specific DNA in human tumors. II. Nucleic acid hybridizations with complementary RNA of human herpes group viruses , 1974, International journal of cancer.

[30]  C. Heath The epidemiology of Hodgkin's disease. , 1972, Annals of internal medicine.