Radiation induced abnormalities in early in vitro mouse embryos

Female mice were superovulated and mated, and the two‐cell embryos were collected and cultured in vitro. The embryos were exposed to x‐irradiation (0–491 rads) during the two‐cell stage, before the appearance of the next cleavage plate, placed in new unirradiated culture medium and observed during subsequent development. Morphological abnormalities which occurred as a result of irradiation included fragmentation, disintegration, granulation, incomplete cleavage, cleavage cessation, nuclear degeneration and pycnosis and cytoplasmic vacuolization. There was no damage to the zona pellucida.

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