A new role for the dynamin GTPase in the regulation of fusion pore expansion

The role of dynamin GTPase activity in controlling fusion pore expansion and postfusion granule membrane topology was investigated. The experiments show that, in addition to playing a role in endocytosis, GTPase activity of dynamin regulates the rapidity of fusion pore expansion from tens of milliseconds to seconds after fusion.

[1]  Daniel Axelrod,et al.  Polarized TIRFM Reveals Changes in Plasma Membrane Topology Before and During Granule Fusion , 2010, Cellular and Molecular Neurobiology.

[2]  J. Hartwig,et al.  Direct dynamin–actin interactions regulate the actin cytoskeleton , 2010, The EMBO journal.

[3]  Agustín D. Martínez,et al.  The Association of Dynamin with Synaptophysin Regulates Quantal Size and Duration of Exocytotic Events in Chromaffin Cells , 2010, The Journal of Neuroscience.

[4]  Johann A. Gagnon-Bartsch,et al.  Vesicular Monoamine and Glutamate Transporters Select Distinct Synaptic Vesicle Recycling Pathways , 2010, The Journal of Neuroscience.

[5]  R. Edwards,et al.  Localized topological changes of the plasma membrane upon exocytosis visualized by polarized TIRFM , 2010, The Journal of cell biology.

[6]  Sandra L. Schmid,et al.  Conserved Functions of Membrane Active GTPases in Coated Vesicle Formation , 2009, Science.

[7]  J. Jaiswal,et al.  Exocytosis of Post-Golgi Vesicles Is Regulated by Components of the Endocytic Machinery , 2009, Cell.

[8]  H. McMahon,et al.  Mechanisms of endocytosis. , 2009, Annual review of biochemistry.

[9]  M. Lindau,et al.  F-Actin and Myosin II Accelerate Catecholamine Release from Chromaffin Granules , 2009, The Journal of Neuroscience.

[10]  S. Schmid,et al.  GTPase Cycle of Dynamin Is Coupled to Membrane Squeeze and Release, Leading to Spontaneous Fission , 2008, Cell.

[11]  S. Schmid,et al.  Isoform and splice-variant specific functions of dynamin-2 revealed by analysis of conditional knock-out cells. , 2008, Molecular biology of the cell.

[12]  Corey Smith,et al.  Dynamin I plays dual roles in the activity-dependent shift in exocytic mode in mouse adrenal chromaffin cells. , 2008, Archives of biochemistry and biophysics.

[13]  D. Axelrod,et al.  The structural and functional implications of linked SNARE motifs in SNAP25. , 2008, Molecular biology of the cell.

[14]  S. Schmid,et al.  Real‐time detection reveals that effectors couple dynamin's GTP‐dependent conformational changes to the membrane , 2008, The EMBO journal.

[15]  N. Vitale,et al.  SNARE-catalyzed fusion events are regulated by Syntaxin1A-lipid interactions. , 2007, Molecular biology of the cell.

[16]  V. Murthy,et al.  Inhibition of dynamin completely blocks compensatory synaptic vesicle endocytosis , 2006, Proceedings of the National Academy of Sciences.

[17]  T. Kirchhausen,et al.  Dynasore, a cell-permeable inhibitor of dynamin. , 2006, Developmental cell.

[18]  P. Camilli,et al.  GTP-dependent twisting of dynamin implicates constriction and tension in membrane fission , 2006, Nature.

[19]  D. Sulzer,et al.  Analysis of exocytotic events recorded by amperometry , 2005, Nature Methods.

[20]  Corey Smith,et al.  Activity-Dependent Differential Transmitter Release in Mouse Adrenal Chromaffin Cells , 2005, The Journal of Neuroscience.

[21]  G. Rutter,et al.  Mechanisms of Dense Core Vesicle Recapture following “Kiss and Run” (“Cavicapture”) Exocytosis in Insulin-secreting Cells* , 2004, Journal of Biological Chemistry.

[22]  W. Almers,et al.  Recapture after exocytosis causes differential retention of protein in granules of bovine chromaffin cells , 2004, The Journal of physiology.

[23]  S. Schmid,et al.  Dynamin GTPase Domain Mutants That Differentially Affect GTP Binding, GTP Hydrolysis, and Clathrin-mediated Endocytosis* , 2004, Journal of Biological Chemistry.

[24]  T. A. Ryan,et al.  The Kinetics of Synaptic Vesicle Pool Depletion at CNS Synaptic Terminals , 2004, Neuron.

[25]  Mica Ohara-Imaizumi,et al.  Secretory granules are recaptured largely intact after stimulated exocytosis in cultured endocrine cells , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[26]  P. De Camilli,et al.  Imaging direct, dynamin-dependent recapture of fusing secretory granules on plasma membrane lawns from PC12 cells , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[27]  H. McMahon,et al.  Dynamin-dependent and dynamin-independent processes contribute to the regulation of single vesicle release kinetics and quantal size , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[28]  H. Palfrey,et al.  Sustained stimulation shifts the mechanism of endocytosis from dynamin-1-dependent rapid endocytosis to clathrin- and dynamin-2-mediated slow endocytosis in chromaffin cells , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[29]  P. De Camilli,et al.  Dynamin at actin tails , 2002, Proceedings of the National Academy of Sciences of the United States of America.

[30]  M. Jackson,et al.  Synaptotagmin Modulation of Fusion Pore Kinetics in Regulated Exocytosis of Dense-Core Vesicles , 2001, Science.

[31]  J. Bonifacino,et al.  Adaptor-related proteins. , 2001, Current opinion in cell biology.

[32]  I. Mills,et al.  GTPase activity of dynamin and resulting conformation change are essential for endocytosis , 2001, Nature.

[33]  S. Chasserot-Golaz,et al.  Presence of Dynamin—Syntaxin Complexes Associated with Secretory Granules in Adrenal Chromaffin Cells , 2000, Journal of neurochemistry.

[34]  T. Südhof,et al.  EHSH1/Intersectin, a Protein That Contains EH and SH3 Domains and Binds to Dynamin and SNAP-25 , 1999, The Journal of Biological Chemistry.

[35]  M. Mann,et al.  In mouse brain profilin I and profilin II associate with regulators of the endocytic pathway and actin assembly , 1998, The EMBO journal.

[36]  S. Schmid,et al.  Ubiquitously expressed dynamin-II has a higher intrinsic GTPase activity and a greater propensity for self-assembly than neuronal dynamin-I. , 1997, Molecular biology of the cell.

[37]  G. Alvarez de Toledo,et al.  The exocytotic event in chromaffin cells revealed by patch amperometry , 1997, Nature.

[38]  S. Schmid,et al.  Dynamin Self-assembly Stimulates Its GTPase Activity* , 1996, The Journal of Biological Chemistry.

[39]  W. Almers,et al.  Fast steps in exocytosis and endocytosis studied by capacitance measurements in endocrine cells , 1996, Current Opinion in Neurobiology.

[40]  R. Chow,et al.  Rapid fluctuations in transmitter release from single vesicles in bovine adrenal chromaffin cells. , 1996, Biophysical journal.

[41]  M. McNiven,et al.  Rapid endocytosis coupled to exocytosis in adrenal chromaffin cells involves Ca2+, GTP, and dynamin but not clathrin. , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[42]  S. Schmid,et al.  Dynamin GTPase is stimulated by crosslinking through the C‐terminal proline‐rich domain. , 1995, The EMBO journal.

[43]  S. Schmid,et al.  Dynamin self-assembles into rings suggesting a mechanism for coated vesicle budding , 1995, Nature.

[44]  S. Schmid,et al.  Tubular membrane invaginations coated by dynamin rings are induced by GTP-γS in nerve terminals , 1995, Nature.

[45]  S. Schmid,et al.  Induction of mutant dynamin specifically blocks endocytic coated vesicle formation , 1994, The Journal of cell biology.

[46]  M. Uhler,et al.  Transient transfection studies of secretion in bovine chromaffin cells and PC12 cells. Generation of kainate-sensitive chromaffin cells. , 1993, The Journal of biological chemistry.

[47]  Robert H. Chow,et al.  Delay in vesicle fusion revealed by electrochemical monitoring of single secretory events in adrenal chromaffin cells , 1992, Nature.

[48]  J. A. Jankowski,et al.  Temporally resolved catecholamine spikes correspond to single vesicle release from individual chromaffin cells. , 1991, Proceedings of the National Academy of Sciences of the United States of America.

[49]  P. De Camilli,et al.  Tubular membrane invaginations coated by dynamin rings are induced by GTP-gamma S in nerve terminals. , 1995, Nature.

[50]  J. Hinshaw.  Dynamin and Its Role in Membrane Fission , 2022 .