Partial purification and cleavage specificity of a site-specific endonuclease, SciNI, isolated from Spiroplasma citri

A site-specific endonuclease, SciNI, has been partially purified from the plant pathogen Spiroplasma citri. The enzyme recognizes the sequence 5'-G-C-G-C-3' and cleaves between the first G and C. 3'-C-G-C-G-5' SciNI is an isoschizomer of HhaI, but generates DNA fragments with 5' rather than 3' single-stranded protrusions.

[1]  C R Woese,et al.  The phylogeny of prokaryotes. , 1980, Microbiological sciences.

[2]  R J Roberts,et al.  Restriction and modification enzymes and their recognition sequences. , 1983, Nucleic acids research.

[3]  W. Gilbert,et al.  Sequencing end-labeled DNA with base-specific chemical cleavages. , 1980, Methods in enzymology.

[4]  N. Brown,et al.  A general method for defining restriction enzyme cleavage and recognition sites. , 1980, Methods in enzymology.

[5]  B. Vogelstein,et al.  Preparative and analytical purification of DNA from agarose. , 1979, Proceedings of the National Academy of Sciences of the United States of America.

[6]  J. Sutcliffe.  Complete nucleotide sequence of the Escherichia coli plasmid pBR322. , 1979, Cold Spring Harbor symposia on quantitative biology.

[7]  M. Smith,et al.  Site specific endonuclease from Fusobacterium nucleatum , 1979, Nucleic Acids Res..

[8]  J. Sutcliffe,et al.  pBR322 restriction map derived from the DNA sequence: accurate DNA size markers up to 4361 nucleotide pairs long. , 1978, Nucleic acids research.

[9]  D. McConnell,et al.  A restriction enzyme Tha I from the thermophilic mycoplasma Thermoplasma acidophilum. , 1978, Nucleic acids research.

[10]  C. Garon,et al.  Spiroplasmavirus citri 3: propagation, purification, proteins, and nucleic acid. , 1977, Science.

[11]  V. Pirrotta,et al.  A simple, general procedure for purifying restriction endonucleases. , 1977, Nucleic acids research.

[12]  P. Markham,et al.  Isolation and Characterization of a Non-Helical Strain of Spiroplasma citri , 1977 .

[13]  Hamilton O. Smith,et al.  A simple method for DNA restriction site mapping. , 1976, Nucleic acids research.

[14]  R. Roberts,et al.  A specific endonuclease from Haemophilus haemolyticus. , 1976, Journal of molecular biology.

[15]  H. Smith,et al.  Letter: A suggested nomenclature for bacterial host modification and restriction systems and their enzymes. , 1973, Journal of molecular biology.

[16]  N. Scott,et al.  The genes for cytoplasmic ribosomal ribonucleic Acid in higher plants. , 1973, Plant physiology.

[17]  D. Helinski,et al.  Supercoiled circular DNA-protein complex in Escherichia coli: purification and induced conversion to an opern circular DNA form. , 1969, Proceedings of the National Academy of Sciences of the United States of America.