On—Line State and Parameters Estimation Based Measurements of the Glucose in Mixed Culture System

ABSTRACT A cultivation model of mixed culture system is used for on-line parameters and cells, lactate, NH3, poly-β-hydroxybutyrate (PHB) concentrations estimation, based on measurements of the substrate concentration. In this mixed system sugars such as glucose obtained from food processing waste were converted to lactate by Lactobacillius delbrueckii and lactate was converted in turn to poly-β-hydroxybutyrate (PHB) by Ralstonia eutropha in one fermentor as a model system. The proposed procedure is based on the extended Kalman filtering method.

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