Preparation of Cotton-Wool-Like Poly(lactic acid)-Based Composites Consisting of Core-Shell-Type Fibers

In previous works, we reported the fabrication of cotton-wool-like composites consisting of siloxane-doped vaterite and poly(l-lactic acid) (SiVPCs). Various irregularly shaped bone voids can be filled with the composite, which effectively supplies calcium and silicate ions, enhancing the bone formation by stimulating the cells. The composites, however, were brittle and showed an initial burst release of ions. In the present work, to improve the mechanical flexibility and ion release, the composite fiber was coated with a soft, thin layer consisting of poly(d,l-lactic-co-glycolic acid) (PLGA). A coaxial electrospinning technique was used to prepare a cotton-wool-like material comprising “core-shell”-type fibers with a diameter of ~12 µm. The fibers, which consisted of SiVPC coated with a ~2-µm-thick PLGA layer, were mechanically flexible; even under a uniaxial compressive load of 1.5 kPa, the cotton-wool-like material did not exhibit fracture of the fibers and, after removing the load, showed a ~60% recovery. In Tris buffer solution, the initial burst release of calcium and silicate ions from the “core-shell”-type fibers was effectively controlled, and the ions were slowly released after one day. Thus, the mechanical flexibility and ion-release behavior of the composites were drastically improved by the thin PLGA coating.

[1]  Xuebin B. Yang,et al.  Bone tissue engineering by using a combination of polymer/Bioglass composites with human adipose-derived stem cells , 2014, Cell and Tissue Research.

[2]  Julian R. Jones,et al.  Tracking the formation of vaterite particles containing aminopropyl-functionalized silsesquioxane and their structure for bone regenerative medicine. , 2013, Journal of materials chemistry. B.

[3]  A. Obata,et al.  Cellular Migration to Electrospun Poly(Lactic Acid) Fibermats , 2012, Journal of biomaterials science. Polymer edition.

[4]  Julian R. Jones,et al.  Preparation of Electrospun Poly(Lactic Acid)-Based Hybrids Containing Siloxane-Doped Vaterite Particles for Bone Regeneration , 2012, Journal of biomaterials science. Polymer edition.

[5]  A. Obata,et al.  Siloxane-poly(lactic acid)-vaterite composites with 3D cotton-like structure , 2012, Journal of Materials Science: Materials in Medicine.

[6]  Hirenkumar K. Makadia,et al.  Poly Lactic-co-Glycolic Acid ( PLGA ) as Biodegradable Controlled Drug Delivery Carrier , 2011 .

[7]  Aldo R Boccaccini,et al.  A review of the biological response to ionic dissolution products from bioactive glasses and glass-ceramics. , 2011, Biomaterials.

[8]  Yan Sun,et al.  Bioactive Electrospun Scaffolds Delivering Growth Factors and Genes for Tissue Engineering Applications , 2010, Pharmaceutical Research.

[9]  João F. Mano,et al.  Polymer/bioactive glass nanocomposites for biomedical applications: A review , 2010 .

[10]  A. Obata,et al.  Electrospun microfiber meshes of silicon-doped vaterite/poly(lactic acid) hybrid for guided bone regeneration. , 2010, Acta biomaterialia.

[11]  Delbert E. Day,et al.  Glass and Medicine , 2010 .

[12]  X. Mo,et al.  Fabrication and characterization of biodegradable nanofibrous mats by mix and coaxial electrospinning , 2009, Journal of materials science. Materials in medicine.

[13]  J. E. Díaz,et al.  Controlled Encapsulation of Hydrophobic Liquids in Hydrophilic Polymer Nanofibers by Co‐electrospinning , 2006 .

[14]  S. Ramakrishna,et al.  Coaxial electrospinning of (fluorescein isothiocyanate-conjugated bovine serum albumin)-encapsulated poly(epsilon-caprolactone) nanofibers for sustained release. , 2006, Biomacromolecules.

[15]  M. Márquez,et al.  Electrically forced coaxial nanojets for one-step hollow nanofiber design. , 2004, Journal of the American Chemical Society.

[16]  Younan Xia,et al.  Direct Fabrication of Composite and Ceramic Hollow Nanofibers by Electrospinning , 2004 .

[17]  Larry L Hench,et al.  Bioactive glasses for in situ tissue regeneration , 2004, Journal of biomaterials science. Polymer edition.

[18]  Andreas Greiner,et al.  Compound Core–Shell Polymer Nanofibers by Co‐Electrospinning , 2003 .

[19]  M. Kotaki,et al.  A review on polymer nanofibers by electrospinning and their applications in nanocomposites , 2003 .

[20]  M. Buggy,et al.  Bone cements and fillers: A review , 2003, Journal of materials science. Materials in medicine.

[21]  Benjamin Chu,et al.  Structure and morphology changes during in vitro degradation of electrospun poly(glycolide-co-lactide) nanofiber membrane. , 2003, Biomacromolecules.

[22]  Larry L Hench,et al.  Third-Generation Biomedical Materials , 2002, Science.

[23]  L L Hench,et al.  Gene-expression profiling of human osteoblasts following treatment with the ionic products of Bioglass 45S5 dissolution. , 2001, Journal of biomedical materials research.

[24]  J C Middleton,et al.  Synthetic biodegradable polymers as orthopedic devices. , 2000, Biomaterials.

[25]  J. Polak,et al.  Ionic products of bioactive glass dissolution increase proliferation of human osteoblasts and induce insulin-like growth factor II mRNA expression and protein synthesis. , 2000, Biochemical and biophysical research communications.

[26]  W. S. Pietrzak,et al.  Calcium sulfate bone void filler: a review and a look ahead. , 2000, The Journal of craniofacial surgery.

[27]  Julian R Jones,et al.  Review of bioactive glass: from Hench to hybrids. , 2013, Acta biomaterialia.

[28]  A. Obata,et al.  Enhanced in vitro cell activity on silicon-doped vaterite/poly(lactic acid) composites. , 2009, Acta biomaterialia.