Enzymatic Spectrophotometric Determination of Glycerol-3-Phosphate and Glycerophosphorylcholine.

A reliable enzymatic procedure has been devised for the determination of glycerol-3-phosphate and glycero-phosphorylcholine based on spectrophotometry. Glycerol-3-phosphate oxidase and phospholipase-D enzymes are used to produce hydrogen peroxide to oxidize iodide into triiodide at an elevated pH in the presence of Mo(VI), and the absorbance of the triiodide was read at 352 nm..

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