Structure-guided engineering of Anticalins with improved binding behavior and biochemical characteristics for application in radio-immuno imaging and/or therapy.

[1]  Michaela Gebauer,et al.  Combinatorial design of an Anticalin directed against the extra-domain b for the specific targeting of oncofetal fibronectin. , 2013, Journal of molecular biology.

[2]  M. Tomblyn.  Radioimmunotherapy for B-cell non-hodgkin lymphomas. , 2012, Cancer control : journal of the Moffitt Cancer Center.

[3]  D. Goldenberg,et al.  Pretargeted Molecular Imaging and Radioimmunotherapy , 2012, Theranostics.

[4]  W. Mcbride,et al.  Pretargeting: taking an alternate route for localizing radionuclides , 2012, Tumor Biology.

[5]  S. Batra,et al.  Recent trends in antibody-based oncologic imaging. , 2012, Cancer letters.

[6]  M. Sakaguchi,et al.  Positive charges on the translocating polypeptide chain arrest movement through the translocon , 2011, Journal of Cell Science.

[7]  I. Pastan,et al.  Effect of chelator conjugation level and injection dose on tumor and organ uptake of 111In-labeled MORAb-009, an anti-mesothelin antibody. , 2011, Nuclear medicine and biology.

[8]  Zhen Cheng,et al.  Protein scaffold-based molecular probes for cancer molecular imaging , 2011, Amino Acids.

[9]  D. Neri,et al.  Antibody-Radionuclide Conjugates for Cancer Therapy: Historical Considerations and New Trends , 2011, Clinical Cancer Research.

[10]  Roland L. Dunbrack,et al.  A smoothed backbone-dependent rotamer library for proteins derived from adaptive kernel density estimates and regressions. , 2011, Structure.

[11]  K Dane Wittrup,et al.  Engineering an antibody with picomolar affinity to DOTA chelates of multiple radionuclides for pretargeted radioimmunotherapy and imaging. , 2011, Nuclear medicine and biology.

[12]  J. Reichert.  Antibody-based therapeutics to watch in 2011 , 2011, mAbs.

[13]  Fredrik Y Frejd,et al.  Affibody molecules: Engineered proteins for therapeutic, diagnostic and biotechnological applications , 2010, FEBS letters.

[14]  Vladimir Tolmachev,et al.  Imaging of EGFR expression in murine xenografts using site-specifically labelled anti-EGFR 111In-DOTA-ZEGFR:2377 Affibody molecule: aspect of the injected tracer amount , 2010, European Journal of Nuclear Medicine and Molecular Imaging.

[15]  S. Koide.  Design And Engineering Of Synthetic Binding Proteins Using Nonantibody Scaffolds , 2009 .

[16]  Michaela Gebauer,et al.  Engineered protein scaffolds as next-generation antibody therapeutics. , 2009, Current opinion in chemical biology.

[17]  P. Kaye,et al.  An engineered lipocalin specific for CTLA-4 reveals a combining site with structural and conformational features similar to antibodies , 2009, Proceedings of the National Academy of Sciences.

[18]  Joachim Feldwisch,et al.  Targeting of HER2-Expressing Tumors with a Site-Specifically 99mTc-Labeled Recombinant Affibody Molecule, ZHER2:2395, with C-Terminally Engineered Cysteine , 2009, Journal of Nuclear Medicine.

[19]  A. Skerra,et al.  High-affinity recognition of lanthanide(III) chelate complexes by a reprogrammed human lipocalin 2. , 2009, Journal of the American Chemical Society.

[20]  Gang Huang,et al.  Methionine oxidation in human IgG2 Fc decreases binding affinities to protein A and FcRn , 2009, Protein science : a publication of the Protein Society.

[21]  Arne Skerra,et al.  Alternative binding proteins: Anticalins – harnessing the structural plasticity of the lipocalin ligand pocket to engineer novel binding activities , 2008, The FEBS journal.

[22]  M. Brechbiel,et al.  Development of radioimmunotherapeutic and diagnostic antibodies: an inside-out view. , 2007, Nuclear medicine and biology.

[23]  K. Henrick,et al.  Inference of macromolecular assemblies from crystalline state. , 2007, Journal of molecular biology.

[24]  A. Skerra.  Alternative non-antibody scaffolds for molecular recognition. , 2007, Current opinion in biotechnology.

[25]  Arne Skerra,et al.  Anticalins as alternative binding proteins for therapeutic use. , 2007, Current opinion in molecular therapeutics.

[26]  Randy J. Read,et al.  Phaser crystallographic software , 2007, Journal of applied crystallography.

[27]  Manfred J. Sippl,et al.  NQ-Flipper: recognition and correction of erroneous asparagine and glutamine side-chain rotamers in protein structures , 2007, Nucleic Acids Res..

[28]  Mingde Zhu,et al.  Kinetic analysis of a high-affinity antibody/antigen interaction performed by multiple Biacore users. , 2006, Analytical biochemistry.

[29]  J. Chatal,et al.  Antibody pretargeting advances cancer radioimmunodetection and radioimmunotherapy. , 2006, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[30]  A. Skerra,et al.  Comparative ligand-binding analysis of ten human lipocalins. , 2006, Biochimica et biophysica acta.

[31]  A. Wu,et al.  Tailoring antibodies for radionuclide delivery , 2006, Expert opinion on drug delivery.

[32]  W. Mcbride,et al.  Improving the Delivery of Radionuclides for Imaging and Therapy of Cancer Using Pretargeting Methods , 2005, Clinical Cancer Research.

[33]  P. Hudson,et al.  Engineered antibody fragments and the rise of single domains , 2005, Nature Biotechnology.

[34]  I. Pastan,et al.  Pretargeted radioimmunotherapy of mesothelin-expressing cancer using a tetravalent single-chain Fv-streptavidin fusion protein. , 2005, Journal of nuclear medicine : official publication, Society of Nuclear Medicine.

[35]  Conrad C. Huang,et al.  UCSF Chimera—A visualization system for exploratory research and analysis , 2004, J. Comput. Chem..

[36]  M. Brechbiel,et al.  Antibody-targeted radiation cancer therapy , 2004, Nature Reviews Drug Discovery.

[37]  Ron D. Appel,et al.  ExPASy: the proteomics server for in-depth protein knowledge and analysis , 2003, Nucleic Acids Res..

[38]  K. Raymond,et al.  Enterobactin: An archetype for microbial iron transport , 2003, Proceedings of the National Academy of Sciences of the United States of America.

[39]  A. Fisher,et al.  A rare earth-DOTA-binding antibody: probe properties and binding affinity across the lanthanide series. , 2003, Journal of the American Chemical Society.

[40]  R. Strong,et al.  The neutrophil lipocalin NGAL is a bacteriostatic agent that interferes with siderophore-mediated iron acquisition. , 2002, Molecular cell.

[41]  Arne Skerra,et al.  Tuning ligand affinity, specificity, and folding stability of an engineered lipocalin variant -- a so-called 'anticalin' -- using a molecular random approach. , 2002, Biophysical chemistry.

[42]  A. Skerra,et al.  Duocalins: Engineered Ligand-Binding Proteins with Dual Specificity Derived from the Lipocalin Fold , 2001, Biological chemistry.

[43]  A. Skerra.  Lipocalins as a scaffold. , 2000, Biochimica et biophysica acta.

[44]  A. Skerra,et al.  A novel type of receptor protein, based on the lipocalin scaffold, with specificity for digoxigenin. , 2000, Journal of molecular biology.

[45]  D. Myszka,et al.  Improving biosensor analysis , 1999, Journal of molecular recognition : JMR.

[46]  A. Fersht.  Structure and mechanism in protein science , 1998 .

[47]  D R Flower,et al.  The lipocalin protein family: structure and function. , 1996, The Biochemical journal.

[48]  C. Sander,et al.  Errors in protein structures , 1996, Nature.

[49]  Tristan J. Vaughan,et al.  Human Antibodies with Sub-nanomolar Affinities Isolated from a Large Non-immunized Phage Display Library , 1996, Nature Biotechnology.

[50]  D. A. Dougherty,et al.  Cation-π Interactions in Chemistry and Biology: A New View of Benzene, Phe, Tyr, and Trp , 1996, Science.

[51]  Collaborative Computational,et al.  The CCP4 suite: programs for protein crystallography. , 1994, Acta crystallographica. Section D, Biological crystallography.

[52]  W. Butler,et al.  How the anti-(metal chelate) antibody CHA255 is specific for the metal ion of its antigen: X-ray structures for two Fab'/hapten complexes with different metals in the chelate. , 1993, Biochemistry.

[53]  J. Thornton,et al.  PROCHECK: a program to check the stereochemical quality of protein structures , 1993 .

[54]  S. Fling,et al.  Peptide and protein molecular weight determination by electrophoresis using a high-molarity tris buffer system without urea. , 1986, Analytical biochemistry.

[55]  A. Skerra,et al.  Anticalins small engineered binding proteins based on the lipocalin scaffold. , 2012, Methods in enzymology.

[56]  Serge X. Cohen,et al.  Automated macromolecular model building for X-ray crystallography using ARP/wARP version 7 , 2008, Nature Protocols.

[57]  Arne Skerra,et al.  The Strep-tag system for one-step purification and high-affinity detection or capturing of proteins , 2007, Nature Protocols.

[58]  W. Delano.  The PyMOL Molecular Graphics System , 2002 .

[59]  Axel T. Brunger,et al.  Free R value: cross-validation in crystallography. , 1997 .