Capabilities of a penetrating microelectrode array for recording single units in dorsal root ganglia of the cat

The recording capability of a microelectrode array in the cat dorsal root ganglion (DRG) was studied in 11 acute experiments, 373 single, discriminable sensory units were recorded on 587 electrodes (0.64 units/electrode). Sensory action potentials as large as 1750 microV were obtained (mean=132 microV). These were comparable to literature reports of the best DRG extracellular recordings made with conventional electrodes. We were able simultaneously to activate and record over 50 discriminable, time-varying units from L6 and L7 DRGs during a cyclic ankle displacement. We also successfully recorded stable, phase dependent multiple sensory units with very little artifact or electromyographic (EMG) contamination during decerebrate walking. Thus, the array is capable of recording more effectively from more DRGs neurons than has been achieved by conventional recording techniques. The recording selectivity and stability of the array, coupled with the large number of neurons that can be recorded simultaneously, provide attractive features for better understanding sensorimotor control principles.

[1]  Y. Matsuda,et al.  Studies on sensory neurons of the mouse with intracellular-recording and horseradish peroxidase-injection techniques. , 1979, Journal of neurophysiology.

[2]  P B Brown,et al.  Cat hindlimb tactile dermatomes determined with single-unit recordings. , 1978, Journal of neurophysiology.

[3]  A. Prochazka,et al.  Muscle spindle discharge in normal and obstructed movements. , 1979, The Journal of physiology.

[4]  R. Stein,et al.  Neural prostheses : replacing motor function after disease or disability , 1992 .

[5]  J. Duysens,et al.  Activity patterns in individual hindlimb primary and secondary muscle spindle afferents during normal movements in unrestrained cats. , 1979, Journal of neurophysiology.

[6]  E. Lewis,et al.  Silicon-substrate microelectrode arrays for parallel recording of neural activity in peripheral and cranial nerves , 1994, IEEE Transactions on Biomedical Engineering.

[7]  P. Matthews.  Where does Sherrington's "muscular sense" originate? Muscles, joints, corollary discharges? , 1982, Annual review of neuroscience.

[8]  R. Stein,et al.  Selective stimulation of cat sciatic nerve using an array of varying-length microelectrodes. , 2001, Journal of neurophysiology.

[9]  A. Cameron,et al.  The electrophysiological and morphological characteristics of feline dorsal root ganglion cells , 1986, Brain Research.

[10]  M. McGlamery.  Mammalian Muscle Receptors and Their Central Actions , 1973 .

[11]  G E Loeb,et al.  Neural signals for command control and feedback in functional neuromuscular stimulation: a review. , 1996, Journal of rehabilitation research and development.

[12]  J. Donoghue,et al.  Neuronal Interactions Improve Cortical Population Coding of Movement Direction , 1999, The Journal of Neuroscience.

[13]  J. Bell,et al.  The structure and function of pacinian corpuscles: A review , 1994, Progress in Neurobiology.

[14]  K. Horch,et al.  A silicon-based, three-dimensional neural interface: manufacturing processes for an intracortical electrode array , 1991, IEEE Transactions on Biomedical Engineering.

[15]  R A Normann,et al.  The Utah intracortical Electrode Array: a recording structure for potential brain-computer interfaces. , 1997, Electroencephalography and clinical neurophysiology.

[16]  B L Munger,et al.  The structure and function of cutaneous sensory receptors. , 1988, Archives of histology and cytology.

[17]  H Burton,et al.  The organization of the seventh lumbar spinal ganglion of the cat , 1973, The Journal of comparative neurology.

[18]  K. Horch,et al.  Biocompatibility of silicon-based electrode arrays implanted in feline cortical tissue. , 1993, Journal of biomedical materials research.

[19]  A. Iggo,et al.  Cutaneous thermoreceptors in primates and sub‐primates , 1969, The Journal of physiology.

[20]  G. E. Loeb,et al.  Single unit conduction velocities from averaged nerve cuff electrode records in freely moving cats , 1981, Journal of Neuroscience Methods.

[21]  W. Kollmann,et al.  Sympathetic and afferent somata projecting in hindlimb nerves and the anatomical organization of the lumbar sympathetic nervous system of the rat , 1988, The Journal of comparative neurology.

[22]  R. Stein,et al.  Stable long-term recordings from cat peripheral nerves , 1977, Brain Research.

[23]  T. R. Shantha,et al.  VIII – DORSAL ROOT GANGLION CELLS , 1970 .

[24]  K. S Guillory,et al.  A 100-channel system for real time detection and storage of extracellular spike waveforms , 1999, Journal of Neuroscience Methods.

[25]  A. Prochazka,et al.  Discharges of single hindlimb afferents in the freely moving cat. , 1976, Journal of neurophysiology.

[26]  R. Stein,et al.  Regeneration Electrode Units: Implants for Recording from Single Peripheral Nerve Fibers in Freely Moving Animals , 1974, Science.

[27]  A. Branner,et al.  A multielectrode array for intrafascicular recording and stimulation in sciatic nerve of cats , 2000, Brain Research Bulletin.

[28]  R A Normann,et al.  Chronic intracortical microstimulation (ICMS) of cat sensory cortex using the Utah Intracortical Electrode Array. , 1999, IEEE transactions on rehabilitation engineering : a publication of the IEEE Engineering in Medicine and Biology Society.

[29]  R. Normann,et al.  Chronic recording capability of the Utah Intracortical Electrode Array in cat sensory cortex , 1998, Journal of Neuroscience Methods.

[30]  D. J. Warren,et al.  A neural interface for a cortical vision prosthesis , 1999, Vision Research.

[31]  W. Willis,et al.  Sensory Mechanisms of the Spinal Cord , 1991, Springer US.

[32]  Craig T. Nordhausen,et al.  Single unit recording capabilities of a 100 microelectrode array , 1996, Brain Research.

[33]  P. Grigg,et al.  Response of primate joint afferent neurons to mechanical stimulation of knee joint. , 1977, Journal of neurophysiology.

[34]  R B Stein,et al.  Phasic and tonic modulation of impulse rates in gamma-motoneurons during locomotion in premammillary cats. , 1984, Journal of neurophysiology.

[35]  Richard A. Johnson,et al.  Applied Multivariate Statistical Analysis , 1983 .

[36]  G. E. Loeb,et al.  Cuff electrodes for chronic stimulation and recording of peripheral nerve activity , 1996, Journal of Neuroscience Methods.

[37]  W. Jänig,et al.  The cell bodies of origin of sympathetic and sensory axons in some skin and muscle nerves of the cat hindlimb , 1983, The Journal of comparative neurology.

[38]  P. Whelan.  CONTROL OF LOCOMOTION IN THE DECEREBRATE CAT , 1996, Progress in Neurobiology.

[39]  K. Horch,et al.  Closed-loop control of ankle position using muscle afferent feedback with functional neuromuscular stimulation , 1996, IEEE Transactions on Biomedical Engineering.

[40]  J. Duysens,et al.  Long-Term Unit Recording from Somatosensory Neurons in the Spinal Ganglia of the Freely Walking Cat , 1977 .