CORRIGENDUM Glutamine modulates acute dextran sulphate sodium-induced changes in small-intestinal intraepithelial gd -T-lymphocyte expression in mice – CORRIGENDUM

, The present study investigated the effect of glutamine (GLN) on dextran sulphate sodium (DSS)-induced changes in the expression of small-intestinal intraepithelial lymphocyte (IEL) gd -T cells in mice. Mice were randomly assigned to a normal control (NC) group and two DSS-treated groups. The NC group and one of the DSS-treated groups (DSS-C) were fed a common semi-purified diet, while the other DSS-treated group (DSS-G) was fed an identical diet, except that part of casein was replaced by GLN, which provided 25 % of the total amino acid nitrogen. After being fed the diets for 5 d, mice in the NC group then received distilled water, while the DSS groups were treated with distilled water containing 2·5 % DSS for 5d. At the end of the experiment, mice were killed. The small-intestinal IEL gd -T-cell subset was isolated for further analysis. The results showed that DSS treatment resulted in a lower percentage of small-intestinal IEL gd -T cells and higher mRNA expressions of interferon- g , TNF- a , IL-17, complement 5a receptor and keratinocyte growth factor by IEL gd -T cells. GLN administration enhanced the proportion of small-intestinal IEL gd -T cells, and immunomodulatory mediator genes expressed by IEL gd -T cells were lower in DSS-treated mice. The histological findings showed that the immunoreactive intensity of tight junction protein ZO-1 expression in the small-intestinal mucosa was higher in the DSS-G group than in the DSS-C group. These results indicated that pretreatment with GLN increased the proportion of small-intestinal IEL gd -T cells, and down-regulated gd -T-cell-expressed inflammatory mediators that may consequently ameliorate the severity of DSS-induced small-intestinal epithelial injury. The authors apologise for the error.