INCREASED INDUSTRIAL ENZYME PRODUCTION BY PENICILLIUM LILACINUM IBGE 04 USING SHAKEN FLASK TECHNIQUE OF SUBMERGED FERMENTATION

In this work optimization parameters for maximum production of alpha-amylase by Penicillium lilacinum IBGE 04 in submerged fermentation were studied. Various agricultural based by-products (sunflower waste, cotton stalk, rice husk, date syrup and molasses) as carbon sources and various nitrogen sources (corn steep, casein, potassium nitrate, albumin, ammonium sulphate, urea and yeast extract) were used for increased production. Optimal conditions for the production of αamylase (7.68 U/mL) by Penicillium lilacinum IBGE 04 were observed when the strain was grown on culture medium (M1) containing yeast extract as a source of nitrogen, molasses as a source of carbon after 96 h of incubation at 40° C, initial pH 7.5, inoculum size of 6x10 6 conidia in 50 mL of culture medium and agitation rate of 200 rev/min. The strain was proved thermo (up to 60° C) and pH (up to 9.0) stable so it might be a potential strain for industrial utilization.