Methods in cell separations.

Research in the field of cell biology and biomedicine relies on technologies that fractionate cell populations and isolate rare cell types to high purity. A brief overview of methods and commercially available products currently used in cell separations is presented. Cell fractionation by size and density and highly selective affinity-based technologies such as affinity chromatography, fluorescence-activated cell sorting (FACS) and magnetic cell sorting are discussed in terms of throughput, yield, and purity.

[1]  G. Martinelli,et al.  Concomitant mobilization of plasma cells and hematopoietic progenitors into peripheral blood of multiple myeloma patients: positive selection and transplantation of enriched CD34+ cells to remove circulating tumor cells. , 1996, Blood.

[2]  J. Chalmers,et al.  Flow Through, Immunomagnetic Cell Separation , 1998, Biotechnology progress.

[3]  O. Fodstad,et al.  Rapid enrichment and detection of melanoma cells from peripheral blood mononuclear cells by a new assay combining immunomagnetic cell sorting and immunocytochemical staining. , 2001, Recent results in cancer research. Fortschritte der Krebsforschung. Progres dans les recherches sur le cancer.

[4]  G. Edelman,et al.  Cell fractionation and arrangement on fibers, beads, and surfaces. , 1971, Proceedings of the National Academy of Sciences of the United States of America.

[5]  J. Chalmers,et al.  Evaluation of Eluents from Separations of CD34+ Cells from Human Cord Blood Using a Commerical, Immunomagnetic Cell Separation System , 2001, Biotechnology progress.

[6]  Bernd Westphal,et al.  Autologous bone-marrow stem-cell transplantation for myocardial regeneration , 2003, The Lancet.

[7]  G. Meisenholder,et al.  POSTMODERN CULTURE : MAXIMIZING CELL CULTURE OUTPUT AT EVERY LEVEL , 1999 .

[8]  Kenneth D. McClatchey,et al.  Clinical laboratory medicine , 1994 .

[9]  A. Tsukamoto,et al.  Development of a clinically applicable high-speed flow cytometer for the isolation of transplantable human hematopoietic stem cells. , 1995, Journal of hematotherapy.

[10]  R. G. Kroll,et al.  The use of immobilized lectins in the separation of Staphylococcus aureus, Escherichia coli, Listeria and Salmonella spp. from pure cultures and foods. , 1992, The Journal of applied bacteriology.

[11]  A. Grañena,et al.  Combined positive and negative cell selection from allogeneic peripheral blood progenitor cells (PBPC) by use of immunomagnetic methods , 2001, Bone Marrow Transplantation.

[12]  F. Alderuccio,et al.  Mechanisms and applications of stem cell gene therapy in autoimmunity , 2006 .

[13]  H. Zola,et al.  Isolation of antigen‐specific B cells , 2003, Immunology and cell biology.

[14]  E. Shpall,et al.  Transplantation of enriched CD34-positive autologous marrow into breast cancer patients following high-dose chemotherapy: influence of CD34-positive peripheral-blood progenitors and growth factors on engraftment. , 1994, Journal of clinical oncology : official journal of the American Society of Clinical Oncology.

[15]  B. Barlogie,et al.  Purified CD34+ Lin- Thy+ stem cells do not contain clonal myeloma cells. , 1995, Blood.

[16]  P. Stenstad,et al.  Preparation and Application of Monosized Magnetic Particles in Selective Cell Separation , 1997 .

[17]  H. Gross,et al.  Model study detecting breast cancer cells in peripheral blood mononuclear cells at frequencies as low as 10(-7). , 1995, Proceedings of the National Academy of Sciences of the United States of America.

[18]  R. Handgretinger,et al.  Large-scale isolation of CD133+ progenitor cells from G-CSF mobilized peripheral blood stem cells , 2003, Bone Marrow Transplantation.

[19]  P. Cardot,et al.  Sedimentation field-flow-fractionation: emergence of a new cell separation methodology. , 2000, Talanta.

[20]  R. Andrews,et al.  Engraftment after infusion of CD34+ marrow cells in patients with breast cancer or neuroblastoma. , 1991, Blood.

[21]  R. Storb,et al.  Transplantation of allogeneic CD34+ peripheral blood stem cells in patients with advanced hematologic malignancy. , 1996, Blood.

[22]  T. Luft,et al.  Comparison of purity and enrichment of CD34+ cells from bone marrow, umbilical cord and peripheral blood (primed for apheresis) using five separation systems , 1995, Stem cells.

[23]  R. Knuechel,et al.  Evaluation of membrane physiology following fluorescence activated or magnetic cell separation. , 1999, Cytometry.

[24]  CD34: structure, biology, and clinical utility. , 1996, Blood.

[25]  G. van den Engh,et al.  High-speed cell sorting: fundamentals and recent advances. , 2003, Current opinion in biotechnology.

[26]  Tuija Kekarainen,et al.  Optimization of immunomagnetic separation for cord blood-derived hematopoietic stem cells , 2006, BMC Cell Biology.

[27]  W. Bensinger,et al.  Positive selection of viable cell populations using avidin-biotin immunoadsorption. , 1986, Journal of immunological methods.

[28]  R G Ashcroft,et al.  Commercial high speed machines open new opportunities in high throughput flow cytometry (HTFC). , 2000, Journal of immunological methods.

[29]  B. Andersson,et al.  Cell separation on antigen-coated columns. Elimination of high rate antibody-forming cells and immunological memory cells. , 1969 .

[30]  G. I. Bell Models for the specific adhesion of cells to cells. , 1978, Science.

[31]  J. Kearney,et al.  AC133, a novel marker for human hematopoietic stem and progenitor cells. , 1997, Blood.

[32]  Bo Mattiasson,et al.  Affinity fractionation of lymphocytes using a monolithic cryogel. , 2003, Journal of immunological methods.

[33]  D. Cliver,et al.  Immunomagnetic capture PCR to detect viable Cryptosporidium parvum oocysts from environmental samples , 1997, Applied and environmental microbiology.

[34]  S. Rutella,et al.  Clinical isolation and functional characterization of cord blood CD133+ hematopoietic progenitor cells , 2004, Transfusion.

[35]  B. Mattiasson,et al.  Effect of matrix elasticity on affinity binding and release of bioparticles. Elution of bound cells by temperature-induced shrinkage of the smart macroporous hydrogel. , 2007, Langmuir : the ACS journal of surfaces and colloids.

[36]  P. Truffa-bachi,et al.  Specific separation of cells on affinity columns. , 1970, Proceedings of the National Academy of Sciences of the United States of America.

[37]  Kutty Selva Nandakumar,et al.  Affinity binding of cells to cryogel adsorbents with immobilized specific ligands: effect of ligand coupling and matrix architecture , 2005, Journal of molecular recognition : JMR.

[38]  R. Bataille,et al.  G‐CSF alone mobilizes sufficient peripheral blood CD34+ cells for positive selection in newly diagnosed patients with myeloma and lymphoma , 1996, British journal of haematology.

[39]  P. Delves,et al.  The Immune System , 2000 .

[40]  K. Kawa,et al.  Allogeneic hematopoietic transplantation of CD34+ selected cells from an HLA haplo-identical related donor. A long-term follow-up of 135 patients and a comparison of stem cell source between the bone marrow and the peripheral blood , 2000, Bone Marrow Transplantation.

[41]  H. Einsele,et al.  Transplantation of a combination of CD133+ and CD34+ selected progenitor cells from alternative donors , 2004, British journal of haematology.

[42]  B. Mattiasson,et al.  Detachment of affinity-captured bioparticles by elastic deformation of a macroporous hydrogel. , 2006, Proceedings of the National Academy of Sciences of the United States of America.

[43]  A. Weiss,et al.  Detection and characterization of carcinoma cells in the blood. , 1998, Proceedings of the National Academy of Sciences of the United States of America.

[44]  T. Klingebiel,et al.  Isolation of highly purified autologous and allogeneic peripheral CD34+ cells using the CliniMACS device. , 1999, Journal of hematotherapy.

[45]  I. Weissman,et al.  Transplantation of highly purified CD34+Thy-1+ hematopoietic stem cells in patients with metastatic breast cancer. , 2000, Biology of blood and marrow transplantation : journal of the American Society for Blood and Marrow Transplantation.

[46]  E. Gelfand,et al.  Isolation and characterization of human antigen-specific B lymphocytes. , 1994, Clinical immunology and immunopathology.

[47]  R. D. de Wildt,et al.  A new method for the analysis and production of monoclonal antibody fragments originating from single human B cells. , 1997, Journal of immunological methods.

[48]  M. Roederer,et al.  The history and future of the fluorescence activated cell sorter and flow cytometry: a view from Stanford. , 2002, Clinical chemistry.

[49]  Paul H. Bessette,et al.  Marker-specific sorting of rare cells using dielectrophoresis. , 2005, Proceedings of the National Academy of Sciences of the United States of America.

[50]  E. Seifried,et al.  Autologous transplantation of CD133 selected hematopoietic progenitor cells in a pediatric patient with relapsed leukemia , 2002, Bone Marrow Transplantation.

[51]  H Pertoft,et al.  Fractionation of cells and subcellular particles with Percoll. , 2000, Journal of biochemical and biophysical methods.

[52]  A. Bøyum,et al.  Isolation of mononuclear cells and granulocytes from human blood. , 1968 .

[53]  M. Reth Antigen receptors on B lymphocytes. , 1992, Annual review of immunology.

[54]  M. Uhlén,et al.  An integrated system using immunomagnetic separation, polymerase chain reaction, and colorimetric detection for diagnosis of Plasmodium falciparum. , 1997, The American journal of tropical medicine and hygiene.

[55]  A. Lindberg,et al.  Detection of Shigella dysenteriae type 1 and Shigella flexneri in feces by immunomagnetic isolation and polymerase chain reaction , 1992, Journal of clinical microbiology.

[56]  W. Bensinger,et al.  Elimination of Daudi lymphoblasts from human bone marrow using avidin-biotin immunoadsorption. , 1986, Blood.

[57]  E. Holtzman,et al.  The clinical utility of fetal cell sorting to determine prenatally fetal E/e or e/e Rh genotype from peripheral maternal blood. , 2000, American journal of obstetrics and gynecology.

[58]  N. Schmitz,et al.  Purging Peripheral Blood Progenitor Cell Grafts from Lymphoma Cells: Quantitative Comparison of Immunomagnetic CD34+ Selection Systems , 1997, Stem cells.

[59]  Urs O. Häfeli,et al.  Scientific and clinical applications of magnetic carriers , 1997 .

[60]  M. Baccarani,et al.  Phenotypic characterization of immunomagnetically purified umbilical cord blood CD34+ cells. , 1999, Blood cells, molecules & diseases.

[61]  M. Antonietti,et al.  Porous polymers and resins for biotechnological and biomedical applications. , 2002, Journal of biotechnology.

[62]  Robert S Negrin,et al.  Hematopoietic stem and progenitor cells: clinical and preclinical regeneration of the hematolymphoid system. , 2005, Annual review of medicine.

[63]  L. Arseniev,et al.  Transplantation of allogeneic CD34+ blood cells. , 1996, Blood.

[64]  J. Schrader,et al.  A new, simple method for the preparation of lymphocytes bearing specific receptors , 1974, European journal of immunology.

[65]  George M Whitesides,et al.  Polyvalent Interactions in Biological Systems: Implications for Design and Use of Multivalent Ligands and Inhibitors. , 1998, Angewandte Chemie.

[66]  I. Safarik,et al.  Use of magnetic techniques for the isolation of cells. , 1999, Journal of chromatography. B, Biomedical sciences and applications.

[67]  P. Gustavsson,et al.  Continuous superporous agarose beds for chromatography and electrophoresis. , 1999, Journal of chromatography. A.

[68]  F Beaujean,et al.  Methods of CD34+ cell separation: comparative analysis. , 1997, Transfusion Science.

[69]  C. Peters,et al.  Hematopoietic cell transplantation for inherited metabolic diseases: an overview of outcomes and practice guidelines , 2003, Bone Marrow Transplantation.

[70]  J. Hatzfeld,et al.  An improved panning technique for the selection of CD34+ human bone marrow hematopoietic cells with high recovery of early progenitors. , 1995, Experimental hematology.

[71]  D. Collins,et al.  T-lymphocyte functionality assessed by analysis of cytokine receptor expression, intracellular cytokine expression, and femtomolar detection of cytokine secretion by quantitative flow cytometry. , 1998, Cytometry.

[72]  C. Chapman,et al.  Single centre experience of umbilical cord stem cell transplantation for primary immunodeficiency , 2005, Bone Marrow Transplantation.