Expanding the diversity of Chardonnay aroma through the metabolic interactions of Saccharomyces cerevisiae cocultures

Yeast co-inoculations in winemaking are often studied in the framework of modulating the aromatic profiles of wines. Our study aimed to investigate the impact of three cocultures and corresponding pure cultures of Saccharomyces cerevisiae on the chemical composition and the sensory profile of Chardonnay wine. Coculture makes it possible to obtain completely new aromatic expressions that do not exist in the original pure cultures attributed to yeast interactions. Esters, fatty acids and phenol families were identified as affected. The sensory profiles and metabolome of the cocultures, corresponding pure cultures and associated wine blends from both pure cultures were found to be different. The coculture did not turn out to be the addition of the two pure culture wines, indicating the impact of interaction. High resolution mass spectrometry revealed thousands of cocultures biomarkers. The metabolic pathways involved in these wine composition changes were highlighted, most of them belonging to nitrogen metabolism.

[1]  F. Bordet,et al.  eGFP Gene Integration in HO: A Metabolomic Impact? , 2022, Microorganisms.

[2]  Jordi Ballester,et al.  Different Wines from Different Yeasts? “Saccharomyces cerevisiae Intraspecies Differentiation by Metabolomic Signature and Sensory Patterns in Wine” , 2021, Microorganisms.

[3]  Evangelia A. Zilelidou,et al.  Understanding Wine through Yeast Interactions , 2021, Microorganisms.

[4]  M. Ciani,et al.  Yeast Interactions and Molecular Mechanisms in Wine Fermentation: A Comprehensive Review , 2021, International journal of molecular sciences.

[5]  D. Block,et al.  Nitrogenous Compound Utilization and Production of Volatile Organic Compounds among Commercial Wine Yeasts Highlight Strain-Specific Metabolic Diversity , 2021, bioRxiv.

[6]  Raquel Garcia,et al.  Revealing the yeast modulation potential on amino acid composition and volatile profile of Arinto white wines by a combined chromatographic-based approach. , 2021, Journal of chromatography. A.

[7]  A. Mendes-Ferreira,et al.  Phenotypic and transcriptional analysis of Saccharomyces cerevisiae during wine fermentation in response to nitrogen nutrition and co-inoculation with Torulaspora delbrueckii. , 2020, Food research international.

[8]  D. Rossouw,et al.  Real-time monitoring of population dynamics and physical interactions in a synthetic yeast ecosystem by use of multicolour flow cytometry , 2020, Applied Microbiology and Biotechnology.

[9]  P. Schmitt‐Kopplin,et al.  Exploring yeast interactions through metabolic profiling , 2020, Scientific Reports.

[10]  F. Bordet,et al.  Yeast–Yeast Interactions: Mechanisms, Methodologies and Impact on Composition , 2020, Microorganisms.

[11]  Minoru Yoshida,et al.  Intra-species cell-cell communication in yeast. , 2019, FEMS yeast research.

[12]  P. Schmitt‐Kopplin,et al.  Influence of cell-cell contact between L. thermotolerans and S. cerevisiae on yeast interactions and the exo-metabolome. , 2019, Food microbiology.

[13]  M. Larsen,et al.  Proteomics insights into the responses of Saccharomyces cerevisiae during mixed-culture alcoholic fermentation with Lachancea thermotolerans. , 2019, FEMS microbiology ecology.

[14]  A. Querol,et al.  Dominance of wine Saccharomyces cerevisiae strains over S. kudriavzevii in industrial fermentation competitions is related to an acceleration of nutrient uptake and utilization , 2019, Environmental microbiology.

[15]  M. Morales,et al.  Influence of Saccharomyces cerevisiae and Lachancea thermotolerans co-inoculation on volatile profile in fermentations of a must with a high sugar content. , 2019, Food chemistry.

[16]  F. Pinu.  Grape and Wine Metabolomics to Develop New Insights Using Untargeted and Targeted Approaches , 2018, Fermentation.

[17]  C. Cassino,et al.  Managing wine quality using Torulaspora delbrueckii and Oenococcus oeni starters in mixed fermentations of a red Barbera wine , 2018, European Food Research and Technology.

[18]  Thibault Nidelet,et al.  Investigations of the mechanisms of interactions between four non-conventional species with Saccharomyces cerevisiae in oenological conditions , 2018, bioRxiv.

[19]  G. Yan,et al.  Use of Torulaspora delbrueckii Co-fermentation With Two Saccharomyces cerevisiae Strains With Different Aromatic Characteristic to Improve the Diversity of Red Wine Aroma Profile , 2018, Front. Microbiol..

[20]  A. Bloem,et al.  Altered Fermentation Performances, Growth, and Metabolic Footprints Reveal Competition for Nutrients between Yeast Species Inoculated in Synthetic Grape Juice-Like Medium , 2018, Front. Microbiol..

[21]  J. Curiel,et al.  Different Non-Saccharomyces Yeast Species Stimulate Nutrient Consumption in S. cerevisiae Mixed Cultures , 2017, Front. Microbiol..

[22]  Á. Peña-Neira,et al.  Effect of different ageing techniques on the polysaccharide and phenolic composition and sensorial characteristics of Chardonnay white wines fermented with different selected Saccharomyces Cerevisiae yeast strains , 2016, European Food Research and Technology.

[23]  Chunxia Wang,et al.  Fungal diversity in grape must and wine fermentation assessed by massive sequencing, quantitative PCR and DGGE , 2015, Front. Microbiol..

[24]  G. de Revel,et al.  Increase of fruity aroma during mixed T. delbrueckii/S. cerevisiae wine fermentation is linked to specific esters enhancement. , 2015, International journal of food microbiology.

[25]  A. Capece,et al.  Assessment of competition in wine fermentation among wild Saccharomyces cerevisiae strains isolated from Sangiovese grapes in Tuscany region , 2013 .

[26]  Z. Salvadó,et al.  The Fitness Advantage of Commercial Wine Yeasts in Relation to the Nitrogen Concentration, Temperature, and Ethanol Content under Microvinification Conditions , 2013, Applied and Environmental Microbiology.

[27]  Jordi Ballester,et al.  Yeast-yeast interactions revealed by aromatic profile analysis of Sauvignon Blanc wine fermented by single or co-culture of non-Saccharomyces and Saccharomyces yeasts. , 2012, Food microbiology.

[28]  C. Varela,et al.  Effect of ammonium nitrogen supplementation of grape juice on wine volatiles and non-volatiles composition of the aromatic grape variety Albariño , 2012 .

[29]  M. Malfeito-Ferreira,et al.  The microbial ecology of wine grape berries. , 2012, International journal of food microbiology.

[30]  D. Dubourdieu,et al.  The grape must non-Saccharomyces microbial community: impact on volatile thiol release. , 2011, International journal of food microbiology.

[31]  V. Ferreira,et al.  Evolution of the aroma composition of wines supplemented with grape flavour precursors from different varietals during accelerated wine ageing , 2010 .

[32]  I. Mannazzu,et al.  Controlled mixed culture fermentation: a new perspective on the use of non-Saccharomyces yeasts in winemaking. , 2010, FEMS yeast research.

[33]  M. Goddard,et al.  Co-fermentation with "Pichia Kluyveri" increases varietal thiol concentrations in Sauvignon Blanc , 2009 .

[34]  E. King,et al.  Coinoculated fermentations using saccharomyces yeasts affect the volatile composition and sensory properties of Vitis vinifera L. cv. sauvignon blanc wines. , 2008, Journal of agricultural and food chemistry.

[35]  P. Silcock,et al.  Control of Ester Synthesis During Brewery Fermentation , 2008 .

[36]  K. Verstrepen,et al.  Parameters Affecting Ethyl Ester Production by Saccharomyces cerevisiae during Fermentation , 2007, Applied and Environmental Microbiology.

[37]  V. Ferreira,et al.  Release and formation of varietal aroma compounds during alcoholic fermentation from nonfloral grape odorless flavor precursors fractions. , 2007, Journal of agricultural and food chemistry.

[38]  Jane M. McCarthy,et al.  Hydrolysis and transformation of grape glycosidically bound volatile compounds during fermentation with three Saccharomyces yeast strains. , 2006, Journal of agricultural and food chemistry.

[39]  Daniel Cozzolino,et al.  Metabolic profiling as a tool for revealing Saccharomyces interactions during wine fermentation. , 2006, FEMS yeast research.

[40]  J. Sarry,et al.  Plant and microbial glycoside hydrolases: Volatile release from glycosidic aroma precursors , 2004 .

[41]  G. Fleet.  Yeast interactions and wine flavour. , 2003, International journal of food microbiology.

[42]  M. Aznar,et al.  Determination of minor and trace volatile compounds in wine by solid-phase extraction and gas chromatography with mass spectrometric detection. , 2002, Journal of chromatography. A.

[43]  V. Ferreira,et al.  Fast analysis of important wine volatile compounds development and validation of a new method based on gas chromatographic-flame ionisation detection analysis of dichloromethane microextracts. , 2001, Journal of chromatography. A.