Molecular Analysis of H Antigens Reveals that Human DiarrheagenicEscherichia coli O26 Strains That Carry the eaeGene Belong to the H11 Clonal Complex

ABSTRACT Fifty-seven Escherichia coli O26 strains isolated from patients in six countries were investigated by PCR restriction fragment length polymorphism (RFLP) analysis of the flagellin-encoding (fliC) gene (fliC RFLP analysis). The strains were determined by serotyping to belong to five different H types or were nonmotile. The fliC RFLP analysis revealed only two different patterns among the 57 strains. One fliC RFLP pattern was displayed by 54 strains and was identical to that ofE. coli H11 reference strain Su4321-41. The otherfliC RFLP pattern was observed for three strains and was identical to that for E. coli H32 reference strain K10. The 54 strains with the H11 fliC RFLP pattern included 22 strains of serotype O26:H11, 23 nonmotile strains, and 9 strains that were initially serotyped as H2, H8, H21, and H32 but that were confirmed to express H11 by repeat serotyping. All 54 strains with the H11 fliC RFLP pattern contained the attaching-and-effacing (eae) gene. The three strains with the H32 fliCRFLP pattern belonged to serotype O26:H32, and all were eaenegative. The fliC genes of 14 selected E. coliO26:H11 strains isolated between 1964 and 1999 had identical nucleotide sequences. Our results demonstrate that E. coli O26 strains that carry the eae gene belong exclusively to the H11 clonal complex. Since there were no H11 fliC allelic variations among the O26 strains tested, E. coli O26:H11 may have emerged recently. The fliC PCR-RFLP test is a reliable, easy-to-perform, and rapid method for determination of the H types of E. coli O26 isolates.

[1]  M. Frosch,et al.  Escherichia coli O157:H7 and O157:H−Strains That Do Not Produce Shiga Toxin: Phenotypic and Genetic Characterization of Isolates Associated with Diarrhea and Hemolytic-Uremic Syndrome , 1999, Journal of Clinical Microbiology.

[2]  H. Karch,et al.  Epidemiology and diagnosis of Shiga toxin-producing Escherichia coli infections. , 1999, Diagnostic microbiology and infectious disease.

[3]  T. Whittam,et al.  Sequence Diversity of Flagellin (fliC) Alleles in Pathogenic Escherichia coli , 1999, Journal of bacteriology.

[4]  T. Whittam,et al.  Genetic and phenotypic analysis of Escherichia coli with enteropathogenic characteristics isolated from Seattle children. , 1997, The Journal of infectious diseases.

[5]  B. Swaminathan,et al.  Molecular characterization of the gene encoding H antigen in Escherichia coli and development of a PCR-restriction fragment length polymorphism test for identification of E. coli O157:H7 and O157:NM , 1997, Journal of clinical microbiology.

[6]  P. Tarr,et al.  Perspective: the problem of non-O157:H7 shiga toxin (Verocytotoxin)-producing Escherichia coli. , 1996, The Journal of infectious diseases.

[7]  J. Janda,et al.  Verocytotoxin-producing Escherichia coli in children with hemolytic uremic syndrome in the Czech Republic. , 1996, Clinical nephrology.

[8]  A. Caprioli,et al.  Characteristics of Escherichia coli strains belonging to enteropathogenic E. coli serogroups isolated in Italy from children with diarrhea , 1996, Journal of clinical microbiology.

[9]  A. Caprioli,et al.  Genotyping of Shiga-like toxin genes in non-O157 Escherichia coli strains associated with haemolytic uraemic syndrome. , 1995, Journal of medical microbiology.

[10]  J. Heesemann,et al.  Variants of Shiga-like toxin II constitute a major toxin component in Escherichia coli O157 strains from patients with haemolytic uraemic syndrome. , 1994, Journal of medical microbiology.

[11]  C. Whitfield,et al.  Comparative analysis of flagellin sequences from Escherichia coli strains possessing serologically distinct flagellar filaments with a shared complex surface pattern , 1993, Journal of bacteriology.

[12]  T. Whittam,et al.  Clonal relationships among Escherichia coli strains that cause hemorrhagic colitis and infantile diarrhea , 1993, Infection and immunity.

[13]  F. Gunzer,et al.  Molecular detection of sorbitol-fermenting Escherichia coli O157 in patients with hemolytic-uremic syndrome , 1992, Journal of clinical microbiology.

[14]  B. Rowe,et al.  Properties of strains of Escherichia coli O26:H11 in relation to their enteropathogenic or enterohemorrhagic classification. , 1990, The Journal of infectious diseases.

[15]  G. Kuwajima.  Flagellin domain that affects H antigenicity of Escherichia coli K-12 , 1988, Journal of bacteriology.

[16]  H. Karch,et al.  Phage-associated cytotoxin production by and enteroadhesiveness of enteropathogenic Escherichia coli isolated from infants with diarrhea in West Germany. , 1987, The Journal of infectious diseases.

[17]  I. Ørskov,et al.  2 Serotyping of Escherichia coli , 1984 .

[18]  N. Day,et al.  Production of a cytotoxin affecting vero cells by strains of Escherichia coli belonging to traditional enteropathogenic serogroups , 1980 .

[19]  J Konowalchuk,et al.  Vero response to a cytotoxin of Escherichia coli , 1977, Infection and immunity.

[20]  F. Ørskov.  On the occurrence of E. coli belonging to O-group 26 in cases of infantile diarrhoea and white scours. , 1951, Acta pathologica et microbiologica Scandinavica.